The Craft of Peroxisome Purification—A Technical Survey Through the Decades

Islinger, Markus; Manner, Andreas; Völkl, Alfred

doi:10.1007/978-981-13-2233-4_4

Cited by 7 publications

(1 citation statement)

References 167 publications

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“…These properties were effectively exploited by the technique of FFE, whose instrument consisted of a separation chamber with a laminar buffer flow perpendicular to an electric field. Thus, individual particles were deflected from the linear flow in the separation chamber according to their electrophoretic mobility and isoelectric point [ 60 ]. Hence, the separation of similar-density particles was sorted out by using FFE for plant peroxisomes.…”

Section: Crop Subcellular Proteomicsmentioning

confidence: 99%

Crop Proteomics under Abiotic Stress: From Data to Insights

Kausar

Wang

Komatsu

2022

Plants

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Food security is a major challenge in the present world due to erratic weather and climatic changes. Environmental stress negatively affects plant growth and development which leads to reduced crop yields. Technological advancements have caused remarkable improvements in crop-breeding programs. Proteins have an indispensable role in developing stress resilience and tolerance in crops. Genomic and biotechnological advancements have made the process of crop improvement more accurate and targeted. Proteomic studies provide the information required for such targeted approaches. The crosstalk among cellular components is being analyzed by subcellular proteomics. Additionally, the functional diversity of proteins is being unraveled by post-translational modifications during abiotic stress. The exploration of precise cellular responses and the networking among different cellular organelles help in the prediction of signaling pathways and protein–protein interactions. High-throughput mass-spectrometry-based protein studies are now possible due to incremental advancements in mass-spectrometry techniques, sample protocols, and bioinformatic tools as well as the increasing availability of plant genome sequence information for multiple species. In this review, the key role of proteomic analysis in identifying the abiotic-stress-responsive mechanisms in various crops was summarized. The development and availability of advanced computational tools were discussed in detail. The highly variable protein responses among different crops have provided a wide avenue for molecular-marker-assisted genetic buildup studies to develop smart, high-yielding, and stress-tolerant varieties to cope with food-security challenges.

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Section: Crop Subcellular Proteomicsmentioning

confidence: 99%

Crop Proteomics under Abiotic Stress: From Data to Insights

Kausar

Wang

Komatsu

2022

Plants

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Continuous flow electrophoretic separation — Recent developments and applications to biological sample analysis

Št́astná

2019

Electrophoresis

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Continuous flow electrophoretic separation with continuous sample loading provides the advantage of processing volumes of any sizes, as well as the benefit of a real‐time monitoring and optimization of the separation process. In addition, the spatial separation of the sample enables collecting multiple separated components simultaneously and in a continuous manner. The separation is usually performed in mild buffers without organic solvents and detergents (sample biological activity is retained) and it is carried out without usage of a solid support in the separation space preventing the interaction of the sample with it (high sample recovery). The method is used for the separation of proteins/peptides in proteomic applications, and its great applicability is to the separation of the cells, cellular organelles, vesicles, membrane fragments, and DNA. This review focuses on the electrophoretic separation performed in a continuous flow and it describes various electrophoretic modes and instrumental setups. Recent developments in methodology and instrumentation, the integration with other techniques, and the application to the biological sample analysis are discussed as well.

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Isolation of Mammalian Peroxisomes by Density Gradient Centrifugation

Manner¹,

Islinger²

2023

Methods in Molecular Biology

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The Craft of Peroxisome Purification—A Technical Survey Through the Decades

Cited by 7 publications

References 167 publications

Crop Proteomics under Abiotic Stress: From Data to Insights

Crop Proteomics under Abiotic Stress: From Data to Insights

Continuous flow electrophoretic separation — Recent developments and applications to biological sample analysis

Isolation of Mammalian Peroxisomes by Density Gradient Centrifugation

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