The Cre/Lox System: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal Endophytes

Florea, Simona; Machado, Caroline; Andreeva, Kalina; Schardl, Christopher L.

doi:10.1007/978-1-61779-433-9_19

Cited by 2 publications

(1 citation statement)

References 5 publications

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“…nidulans ( Roux and Chooi, 2022 ). In addition, Cre recombinase has been used in Saccharomyces ( Moon et al, 2022 ), Lecanicillium ( Nguyen et al, 2018 ), Trichoderma ( Steiger et al, 2011 ), Neurospora ( Honda and Selker, 2009 ), Neotyphodium ( Florea et al, 2012 ) and so on, setting the foundations for the further development of fungal cell factories.…”

Section: Genome Editing Technologiesmentioning

confidence: 99%

Establishment, optimization, and application of genetic technology in Aspergillus spp.

et al. 2023

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Aspergillus is widely distributed in nature and occupies a crucial ecological niche, which has complex and diverse metabolic pathways and can produce a variety of metabolites. With the deepening of genomics exploration, more Aspergillus genomic informations have been elucidated, which not only help us understand the basic mechanism of various life activities, but also further realize the ideal functional transformation. Available genetic engineering tools include homologous recombinant systems, specific nuclease based systems, and RNA techniques, combined with transformation methods, and screening based on selective labeling. Precise editing of target genes can not only prevent and control the production of mycotoxin pollutants, but also realize the construction of economical and efficient fungal cell factories. This paper reviewed the establishment and optimization process of genome technologies, hoping to provide the theoretical basis of experiments, and summarized the recent progress and application in genetic technology, analyzes the challenges and the possibility of future development with regard to Aspergillus.

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Section: Genome Editing Technologiesmentioning

confidence: 99%

Establishment, optimization, and application of genetic technology in Aspergillus spp.

et al. 2023

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Genetic surgery in fungi: employing site-specific recombinases for genome manipulation

Krappmann

2014

Appl Microbiol Biotechnol

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Site-specific recombination mediates the rearrangement of nucleic acids by the virtue of an recombinase acting on specific recognition sequences. Recombining activities belong either to the tyrosine- or serine-type group, based on the presence of specific residues in the catalytic centre, which can be further subdivided into families due to additional criteria. The most prominent systems are the λ phage integrase acting on att sites; the Cre recombinase from bacteriophage P1 with its loxP attachment sites; the FLP/FTR system of fungal origin, where it is required for 2-μm plasmid replication/amplification in yeast; and the prokaryotic β-recombinase that recombines six sites specifically in cis. Each of these has been exploited in fungal hosts of biotechnological, medical or general relevance, mainly for cloning projects, approaches of gene targeting, genome modification or screening purposes. With their precise and defined mode of action are site-specific recombination systems eminently suited for genetic tasks in fungi, like they are executed in functional studies at high throughput or modern approaches of synthetic biology.

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The Cre/Lox System: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal Endophytes

Cited by 2 publications

References 5 publications

Establishment, optimization, and application of genetic technology in Aspergillus spp.

Establishment, optimization, and application of genetic technology in Aspergillus spp.

Genetic surgery in fungi: employing site-specific recombinases for genome manipulation

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