The cryoprotectant used, its concentration, and the equilibration time are critical for the successful cryopreservation of rabbit sperm: Dimethylacetamide versus dimethylsulfoxide

Iaffaldano, Nicolaia; Iorio, Michele Di; Rosato, M. P.

doi:10.1016/j.theriogenology.2012.06.009

Cited by 42 publications

(48 citation statements)

References 26 publications

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“…Other smallscale experiments using 1 and 2 M Me 2 SO (Hanada andNagase, 1980 andKashiwazaki et al, 2006, respectively) showed no differences between Me 2 SO and acetamide as cryoprotectants for rabbit sperm freezing. On the contrary, several authors have demonstrated the efficient sperm protection of 3.0 and 3.5 M Me 2 SO in rabbit sperm cryopreservation Viudes de Castro and Vicente, 1996;Mocé et al, 2003;Cortell and Viudes de Castro, 2008;Iaffaldano et al, 2012;Rosato and Iaffaldano, 2013). According to our results, when Me 2 SO was used v. acetamide extender, results on frozen-thawed sperm quality and reproductive parameters studied were improved.…”

Section: Discussionsupporting

confidence: 51%

“…It would be highly beneficial in routine artificial insemination (AI) programmes on commercial rabbit farms if semen could be cryopreserved without affecting fertility, although a multitude of factors affect the effectiveness of cryopreservation, including the initial quality of the semen samples, cryopreservation protocol, freezing extenders and environmental factors (Mocé and Vicente, 2009;Iaffaldano et al, 2012;Lavara et al 2013;Rosato and Iaffaldano, 2013). In addition, differences in reproductive parameters have been observed with fresh (Bolet et al, 2000;Vicente et al, 2000) and cryopreserved sperm belonging to different breeds or lines of rabbits.…”

Section: Introductionmentioning

confidence: 99%

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Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Viudes-de-Castro

Lavara

Safaa

et al. 2014

Animal

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This study was conducted to elucidate the effect of different freezing extenders on two lines selected for hyperprolificacy and longevity (H and LP, respectively). In extender A, dimethyl sulphoxide (Me 2 SO) and sucrose were used as cryoprotectants. In extenders B and C, the sucrose was replaced by 20% egg yolk, and in extender C the Me 2 SO was substituted by acetamide. Semen was packaged in 0.25 ml plastic straws and cooled at 5°C for 45 min, and then was frozen in liquid nitrogen vapour for 10 min before being plunged into the liquid nitrogen. Thawing was carried out by immersing the straws in a water bath at 50°C for 10 s. Frozen-thawed semen characteristics and reproductive parameters were affected by freezing. Extender C showed significantly lower post-thawing quality traits than any of the three extenders. Acrosome integrity was significantly improved when Me 2 SO was used as cryoprotectant. Sucrose replacement by 20% egg yolk had no effect on acrosome integrity but provided significantly lower sperm motility and viability. Freezing extender affected fertility rate, total born, number of implantation sites and gestational losses, obtaining better results when extender A was used. The acrosomal integrity after frozen-thawed process showed a significant correlation with fertility at 12 th day and also at birth, indicating that an increase in acrosomal integrity leads to an increase in both fertilities (12 th day and at birth). A positive correlation between motility of semen and implantation sites was found. The post-thawing quality traits of semen were not affected by the genetic line, although LP line showed higher total born and lower foetal and gestational losses. The findings of this study suggest that freezing extender composition has a significant effect on the success of rabbit sperm for preservation, and when Me 2 SO was used as permeable cryoprotectant sucrose provided better protection compared with egg yolk and improved reproductive traits, and, on the other hand, the male genotypes used in the present study had no effect on frozen-thawed sperm parameters but negatively affected some of the reproductive parameters.

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Section: Discussionsupporting

confidence: 51%

Section: Introductionmentioning

confidence: 99%

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Viudes-de-Castro

Lavara

Safaa

et al. 2014

Animal

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“…The cryopreservation techniques for rabbits semen remain suboptimal and the use of frozen-thawed rabbit spermatozoa generally result in lower fertility rates (Moc e & Vicente 2009;Moc e et al 2010;Rosato & Iaffaldano 2011). Only recently, a few reports have shown satisfactory results with frozen semen, nevertheless, the freezing technique is too expensive (Iaffaldano et al 2012;Rosato & Iaffaldano 2013). For these reasons, freezing is not preferred while the preservation of fresh extended semen at temperature of 15-17 C, to preserve vitality, is common in the rabbit industry.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of effectiveness of an innovative semen extender (Formula^®) comparing with a traditional extender (Lepus^®) for artificial insemination in rabbits does

Bresciani

Bianchera

Mazzanti

et al. 2016

Italian Journal of Animal Science

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This study aimed to investigate the preservability and viability of the rabbit spermatozoa diluted in a new semen extender Formula V R in comparison with Lepus V R at 17 C of storage. The main characteristic of the new extender formulation is the use of an enzymatic agent associated to a polysaccharide as energy source precursor, added with gentamycin. During eight trials, ejaculates from 70 bucks were collected and diluted at 1:10 ratio with both the extenders, after 24 h of storage the semen doses were used for the artificial insemination (AI). Aliquots of the semen doses for each trial were stored at 17 C, the total and progressive motility were checked at 0, 4, 12, 18, 24, 36, 48, 60, 72, 84, 96, 108 h of storage. A total of 1267 and 1525 does were inseminated, respectively with Formula V R and Lepus V R . During storage the mean total and progressive motility (77.23% and 72.854%, respectively) were significantly higher for Formula V R (p < .01) and the progressive motility at almost 70% was maintained for at least 60 h vs the 24 h of storage for Lepus V R with significant differences after 12 h of storage (p < .05). The new extender reported a higher pregnancy rate (p < .05) and an average of 9.25 rabbits born per litter vs 8.83 for the traditional extender (p < .05), while the mean of the newborn alive was 9.08 using Formula V R vs 8.51 with Lepus V R (p < .05). In conclusion, the use of Formula V R is recommended for rabbit semen AI programmes. ARTICLE HISTORY

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“…Among the disaccharides used as non-permeable CPAs, sucrose has shown a better cryoprotective effect (Mocé and Vicente, 2009). In previous studies (Iaffaldano et al, 2012;Rosato and Iaffaldano, 2013), we developed an effective freezing protocol for rabbit semen based on the use of DMSO in the freezing extender as the permeable CPA in combination with sucrose.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of rabbit semen using non-permeable cryoprotectants: Effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose

Iaffaldano¹,

Iorio²,

Rosato³

et al. 2014

Animal Reproduction Science

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The cryoprotectant used, its concentration, and the equilibration time are critical for the successful cryopreservation of rabbit sperm: Dimethylacetamide versus dimethylsulfoxide

Cited by 42 publications

References 26 publications

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Evaluation of effectiveness of an innovative semen extender (Formula^®) comparing with a traditional extender (Lepus^®) for artificial insemination in rabbits does

Cryopreservation of rabbit semen using non-permeable cryoprotectants: Effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose

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The cryoprotectant used, its concentration, and the equilibration time are critical for the successful cryopreservation of rabbit sperm: Dimethylacetamide versus dimethylsulfoxide

Cited by 42 publications

References 26 publications

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Evaluation of effectiveness of an innovative semen extender (Formula®) comparing with a traditional extender (Lepus®) for artificial insemination in rabbits does

Cryopreservation of rabbit semen using non-permeable cryoprotectants: Effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose

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Evaluation of effectiveness of an innovative semen extender (Formula^®) comparing with a traditional extender (Lepus^®) for artificial insemination in rabbits does