The Cryoprotective Potential of Propolis Supplemented in Frozen-Thawed Bull Semen: Biochemical and Physiological Findings

Yeni, Deniz; Gülhan, Mehmet Fuat; ̇İnanç, Muhammed Enes; Avdatek, Fatih; Güngör, Salim; Türkmen, Ruhi; Tuncer, Pürhan Barbaros; Taşdemir, Umut

doi:10.21203/rs.3.rs-649334/v1

Cited by 2 publications

(9 citation statements)

References 30 publications

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“…While results about using the PRNL in freezing media are lacking. In the other line, many previous works 10 , 11 , 13 – 17 clarified the protective role of propolis in enhancing the sperm motility, viability, and membrane integrity after cryopreservation in different animal species and fish. Moreover, a significant reduction in the percentages of abnormalities and chromatin damages were observed in our study as similar to previous works 11 , 13 , 16 .…”

Section: Discussionmentioning

confidence: 99%

“…In the other line, many previous works 10 , 11 , 13 – 17 clarified the protective role of propolis in enhancing the sperm motility, viability, and membrane integrity after cryopreservation in different animal species and fish. Moreover, a significant reduction in the percentages of abnormalities and chromatin damages were observed in our study as similar to previous works 11 , 13 , 16 . This improvement in semen quality might ascribe to the potential antioxidant efficiency of new form of PRNL added to semen extender.…”

Section: Discussionmentioning

confidence: 99%

“…Propolis is a natural resinous constituent synthesized by bees that has been extensively applied as a folk medicine since ancient times. Numerous compounds have been recognized in propolis, such as caffeic acid, gallic acid, quercetin, chrysin, galangin and pinocembrin 10 , 11 . Moreover, propolis has been presented to demonstrate strong biological actions, such as antibacterial, antiviral, anticancer, and antioxidant properties 12 .…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, several other studies shown a significant enhance in semen longevity in rabbits 14 , and ram 15 by propolis administration. Additionally, propolis was add to the cryopreserved semen media in fish 16 , goats 17 , bull 11 , ram 12 . The cryoprotective effect of propolis could be due to its ability to promote antioxidant events and significantly decreased the percentages of apoptosis sperm 11 , 12 .…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, propolis was add to the cryopreserved semen media in fish 16 , goats 17 , bull 11 , ram 12 . The cryoprotective effect of propolis could be due to its ability to promote antioxidant events and significantly decreased the percentages of apoptosis sperm 11 , 12 . Certain trials anticipated that the constructive impact of propolis on sperm quality and functionality could be accredited by its favorable unrivaled structural molecules permitting the protection of sperm cells.…”

Section: Introductionmentioning

confidence: 99%

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Effects of propolis-loaded nanoliposomes fortification in extender on buffalo semen cryopreservation

Abdelnour

Hassan

Shehabeldin

et al. 2023

Sci Rep

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Buffalo sperm is sensitive to cryoinjuries, thus improving sperm cryoresistance is a critical approach for wide spreading the assisted reproductive technologies in buffalo. The intention of this work was to assess the effect of propolis-loaded in nanoliposomes (PRNL) supplementation of semen extender on semen quality, antioxidant status and some apoptotic genes of cryopreserved buffalo semen. PRNL were prepared using cholesterol (Chol) as well as soybean lecithin and their physicochemical properties were characterized. Egyptian buffalo bulls (4–6 years) were involved, and the semen samples were collected using the artificial vagina method. Buffalo semen was pooled (n = 25 ejaculates) and cryopreserved in tris extender containing PRNL at 0 (PRNL0), 2 (PRNL2), 4 (PRNL4) and 6 µg/mL (PRNL6), respectively. The PRNL had a size of 113.13 nm and a negative zeta potential (− 56.83 mV). Sperm progressive motility, viability, membrane integrity, abnormalities, chromatin damage, redox status, apoptosis status, and apoptotic genes were investigated after post-thawed buffalo semen. Using 2 or 4 µg/mL PRNL significantly increased sperm progressive motility, viability, and membrane integrity, while sperm abnormalities and the percentage of chromatin damages were the lowest in PRNL2 group. Moreover, the PRNL2 group exhibited the best results for all antioxidative activities (TAC, SOD, GPx and CAT) with significantly higher levels than the other groups (P < 0.05). The levels of ROS and MDA were significantly lower in the PRLN2 compared with other groups. The sperm caspase 3 enzyme activities showed the lowest values in PRNL2 groups followed by PRNL4 and PRNL6 groups with significant differences compared with the control. Adding 2 µg/mL PRNL to freezing media significantly reduced apoptotic genes such as Bax and Caspase 3 in sperm, while significantly increase in Bcl2 expression compared with the control (P < 0.001). The expression of Bcl2, Caspase 3 and Bax genes in sperm were not affected by the 6 µg/mL PRNL addition (P > 0.05). The electron micrography descriptions exemplified that the fortification of 2 or 4 µg/mL PRNL maintained the acrosomal and plasma membrane integrities as well as sustained the ultrastructure integrity of the cryopreserved buffalo spermatozoa when compared with control group, whereas the 6 µg/mL of PRNL demonstrated highest injury to the acrosome and plasma membranes. Results show supplementation of the buffalo freezing extender with 2 or 4 µg/mL of PRNL enhanced post-thawed sperm quality via boosting the antioxidant indices, diminishing the oxidative stress and apoptosis as well as maintained the ultrastructure integrity of frozen-thawed buffalo sperm.

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