The Current Role of Cell-Penetrating Peptides in Cancer Therapy

Feni, Lucia; Neundorf, Ines

doi:10.1007/978-3-319-66095-0_13

Cited by 44 publications

(38 citation statements)

References 104 publications

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“…Therefore, out of necessity, the authors chose to provide only a very broad overview of classification, mechanism of transduction and highlight applications of CPPs as vectors for gene and nucleic acid delivery only. Readers are referred to an earlier review of CPPs by the authors [8] as well as several other excellent reviews [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Cell Penetrating Peptides, Novel Vectors for Gene Therapy

2020

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Cell penetrating peptides (CPPs), also known as protein transduction domains (PTDs), first identified ~25 years ago, are small, 6–30 amino acid long, synthetic, or naturally occurring peptides, able to carry variety of cargoes across the cellular membranes in an intact, functional form. Since their initial description and characterization, the field of cell penetrating peptides as vectors has exploded. The cargoes they can deliver range from other small peptides, full-length proteins, nucleic acids including RNA and DNA, liposomes, nanoparticles, and viral particles as well as radioisotopes and other fluorescent probes for imaging purposes. In this review, we will focus briefly on their history, classification system, and mechanism of transduction followed by a summary of the existing literature on use of CPPs as gene delivery vectors either in the form of modified viruses, plasmid DNA, small interfering RNA, oligonucleotides, full-length genes, DNA origami or peptide nucleic acids.

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Section: Introductionmentioning

confidence: 99%

Cell Penetrating Peptides, Novel Vectors for Gene Therapy

2020

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“…CPPs can be attached to the nanocarrier surface conferring the potential to translocate across the membrane via micropinocytosis. 126,127 CPPs are normally short peptides of 1 to 5 kDa of less than 20 basic amino acids and can be attached to >30 kDa cargoes (eg, TAT). 128 CPP-based drug strategies are considered a form of subcellular targeting due to preferential accumulation in the cell nuclei and sub-nuclear regions.…”

Section: Lectinsmentioning

confidence: 99%

<p>Smart Targeting To Improve Cancer Therapeutics</p>

Morales‐Cruz¹,

Delgado²,

Castillo³

et al. 2019

DDDT

109

101

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Cancer is the second largest cause of death worldwide with the number of new cancer cases predicted to grow significantly in the next decades. Biotechnology and medicine can and should work hand-in-hand to improve cancer diagnosis and treatment efficacy. However, success has been frequently limited, in particular when treating late-stage solid tumors. There still is the need to develop smart and synergistic therapeutic approaches to achieve the synthesis of strong and effective drugs and delivery systems. Much interest has been paid to the development of smart drug delivery systems (drug-loaded particles) that utilize passive targeting, active targeting, and/or stimulus responsiveness strategies. This review will summarize some main ideas about the effect of each strategy and how the combination of some or all of them has shown to be effective. After a brief introduction of current cancer therapies and their limitations, we describe the biological barriers that nanoparticles need to overcome, followed by presenting different types of drug delivery systems to improve drug accumulation in tumors. Then, we describe cancer cell membrane targets that increase cellular drug uptake through active targeting mechanisms. Stimulusresponsive targeting is also discussed by looking at the intra-and extracellular conditions for specific drug release. We include a significant amount of information summarized in tables and figures on nanoparticle-based therapeutics, PEGylated drugs, different ligands for the design of active-targeted systems, and targeting of different organs. We also discuss some still prevailing fundamental limitations of these approaches, eg, by occlusion of targeting ligands.

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“…The ionic structure composition of poly(lysine) peptides may thus diminish its propensity to bind and penetrate cell membranes. However, secondary structure studies of poly(lysine) by electronic and vibrational CD spectroscopy revealed helixto-coil transitions, that were dependent on temperature, solvent, additives and sample concentrations (Drake et al, 1988;Xia and Li, 2011). The apparent helical conformations were characterized as either a left-handed α-helix or a polyproline type II helix which primarily governed the most dominant peptide secondary structure motifs.…”

Section: Poly(lysine)mentioning

confidence: 99%

“…More specifically, CPPs and CTPs are about 4-30 amino acids in length, derived from biologically active motifs found in proteins or from selection techniques that have ushered in unique peptides with the abilities to target cell surface biological markers (e.g., cell surface receptors) and translocate within cells by a variety of cell uptake mechanisms (Habault and Poyet, 2019). As such, CTPs and CPPs have been applied to transport various cargos such as nucleic acids (McClorey and Banerjee, 2018), proteins (Kristensen et al, 2016), imaging probes (Juliano et al, 2009), and drugs (Feni and Neundorf, 2017) into selected cell lines for activity.…”

Section: Introductionmentioning

confidence: 99%

Polyamide Backbone Modified Cell Targeting and Penetrating Peptides in Cancer Detection and Treatment

et al. 2020

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Cell penetrating and targeting peptides (CPPs and CTPs) encompass an important class of biochemically active peptides owning the capabilities of targeting and translocating within selected cell types. As such, they have been widely used in the delivery of imaging and therapeutic agents for the diagnosis and treatment of various diseases, especially in cancer. Despite their potential utility, first generation CTPs and CPPs based on the native peptide sequences are limited by poor biological and pharmacological properties, thereby restricting their efficacy. Therefore, medicinal chemistry approaches have been designed and developed to construct related peptidomimetics. Of specific interest herein, are the design applications which modify the polyamide backbone of lead CTPs and CPPs. These modifications aim to improve the biochemical characteristics of the native peptide sequence in order to enhance its diagnostic and therapeutic capabilities. This review will focus on a selected set of cell penetrating and targeting peptides and their related peptidomimetics whose polyamide backbone has been modified in order to improve their applications in cancer detection and treatment.

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The Current Role of Cell-Penetrating Peptides in Cancer Therapy

Cited by 44 publications

References 104 publications

Cell Penetrating Peptides, Novel Vectors for Gene Therapy

Cell Penetrating Peptides, Novel Vectors for Gene Therapy

<p>Smart Targeting To Improve Cancer Therapeutics</p>

Polyamide Backbone Modified Cell Targeting and Penetrating Peptides in Cancer Detection and Treatment

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