The Current Status and Future Promise of SPR Biosensors

Chiu, Nan Fu

doi:10.3390/bios12110933

Cited by 17 publications

(8 citation statements)

References 24 publications

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“…There are several techniques available for detecting biomolecular interactions. Assays that convert a biochemical binding event into an optical response are considered the most advanced techniques (Chiu, 2022). Surface plasmon resonance (SPR) analysis is one such technique and a versatile tool that is widely used to study biomolecular interactions.…”

Section: Introductionmentioning

confidence: 99%

“…Surface plasmon resonance (SPR) analysis is one such technique and a versatile tool that is widely used to study biomolecular interactions. SPR can determine binding affinity, binding sites, stoichiometry, and real‐time kinetics of association and dissociation between various interactants, including proteins, nucleic acids, polysaccharides, liposomes, glycoproteins, viruses, and cells (Chiu, 2022; Drescher & Drescher, 2023; Kaewpaiboon et al., 2023; Minunni & Bilia, 2009). Measurements can be recorded for the purified interactants or for complex mixtures such as cell culture supernatants and plant extracts (Drescher & Drescher, 2023; Minunni & Bilia, 2009; Schasfoort, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Applications of Surface Plasmon Resonance (SPR) to the Study of Diverse Protein‐Ligand Interactions

Burris,

Gillespie,

Campbell

et al. 2024

Current Protocols

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Functional characterization of enzymes/proteins requires determination of the binding affinity of small molecules or other biomolecules with the target proteins. Several available techniques, such as proteomics and drug discovery strategies, require a precise and high‐throughput assay for rapid and reliable screening of potential candidates for further testing. Surface plasmon resonance (SPR), a well‐established label‐free technique, directly measures biomolecular affinities. SPR assays require immobilization of one interacting component (ligand) on a conductive metal (mostly gold or silver) and a continuous flow of solution containing potential binding partner (analyte) across the surface. The SPR phenomenon occurs when polarized light excites the electrons at the interface of the metal and the dielectric medium to generate electromagnetic waves that propagate parallel to the surface. Changes in the refractive index due to interaction between the ligand and analyte are measured by detecting the reflected light, providing real‐time data on kinetics and specificity. A prominent use of SPR is identifying compounds in crude plant extracts that bind to specific molecules. Procedures that utilize SPR are becoming increasingly applicable outside the laboratory setting, and SPR imaging and localized SPR (LSPR) are cheaper and more portable alternative for in situ detection of plant or mammalian pathogens and drug discovery studies. LSPR, in particular, has the advantage of direct attachment to test tissues in live‐plant studies. Here, we describe three protocols utilizing SPR‐based assays for precise analysis of protein‐ligand interactions. © 2024 Wiley Periodicals LLC.This article was corrected on 06 July 2024. See the end of the full text for details.Basic Protocol 1: SPR comparison of binding affinities of viral reverse transcriptase polymorphismsBasic Protocol 2: SPR screening of crude plant extract for protein‐binding agentsBasic Protocol 3: Localized SPR–based antigen detection using antibody‐conjugated gold nanoparticles

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Applications of Surface Plasmon Resonance (SPR) to the Study of Diverse Protein‐Ligand Interactions

Burris,

Gillespie,

Campbell

et al. 2024

Current Protocols

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“…Surface plasmon resonance (SPR) biosensors have become indispensable tools for POCT of across various biomarker classes due to their user-friendly operation, rapid response times, and exceptional selectivity ( Sun Y. et al, 2022 ). SPR is a powerful and widely adopted technology in biological and chemical sensing, facilitating real-time monitoring of molecular interactions in POCT diagnostics ( Chiu, 2022 ). MNPs have gained recent attention for their integration into SPR systems for biomolecule immobilization and purification.…”

Section: Application Of Mnps-based Biosensor For Poctmentioning

confidence: 99%

Advancements in magnetic nanoparticle-based biosensors for point-of-care testing

Wang,

Jin,

Hang-Mei Leung

et al. 2024

Front. Bioeng. Biotechnol.

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The significance of point-of-care testing (POCT) in early clinical diagnosis and personalized patient care is increasingly recognized as a crucial tool in reducing disease outbreaks and improving patient survival rates. Within the realm of POCT, biosensors utilizing magnetic nanoparticles (MNPs) have emerged as a subject of substantial interest. This review aims to provide a comprehensive evaluation of the current landscape of POCT, emphasizing its growing significance within clinical practice. Subsequently, the current status of the combination of MNPs in the Biological detection has been presented. Furthermore, it delves into the specific domain of MNP-based biosensors, assessing their potential impact on POCT. By combining existing research and spotlighting pivotal discoveries, this review enhances our comprehension of the advancements and promising prospects offered by MNP-based biosensors in the context of POCT. It seeks to facilitate informed decision-making among healthcare professionals and researchers while also promoting further exploration in this promising field of study.

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“…We summarize the characteristics of each exosome isolation method in Table 2 below. SPR biosensors are analytical devices that have been used to monitor binding events between biomolecules, ranging from proteins, cells, exosomes, aptamers, peptides, lipids, and carbohydrates, to nucleic acids [119]. Compared with ELISA, which is the gold standard of immunoassay in clinical practice, SPR biosensors can provide fast and real-time affinity information and/or kinetic data.…”

Section: Isolation Of Exosomesmentioning

confidence: 99%

Advances in Nanoplasmonic Biosensors: Optimizing Performance for Exosome Detection Applications

Nurrohman,

Chiu,

Hsiao

et al. 2024

Biosensors

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The development of sensitive and specific exosome detection tools is essential because they are believed to provide specific information that is important for early detection, screening, diagnosis, and monitoring of cancer. Among the many detection tools, surface-plasmon resonance (SPR) biosensors are analytical devices that offer advantages in sensitivity and detection speed, thereby making the sample-analysis process faster and more accurate. In addition, the penetration depth of the SPR biosensor, which is <300 nm, is comparable to the size of the exosome, making the SPR biosensor ideal for use in exosome research. On the other hand, another type of nanoplasmonic sensor, namely a localized surface-plasmon resonance (LSPR) biosensor, has a shorter penetration depth of around 6 nm. Structural optimization through the addition of supporting layers and gap control between particles is needed to strengthen the surface-plasmon field. This paper summarizes the progress of the development of SPR and LSPR biosensors for detecting exosomes. Techniques in signal amplification from two sensors will be discussed. There are three main parts to this paper. The first two parts will focus on reviewing the working principles of each sensor and introducing several methods that can be used to isolate exosomes. This article will close by explaining the various sensor systems that have been developed and the optimizations carried out to obtain sensors with better performance. To illustrate the performance improvements in each sensor system discussed, the parameters highlighted include the detection limit, dynamic range, and sensitivity.

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The Current Status and Future Promise of SPR Biosensors

Cited by 17 publications

References 24 publications

Applications of Surface Plasmon Resonance (SPR) to the Study of Diverse Protein‐Ligand Interactions

Applications of Surface Plasmon Resonance (SPR) to the Study of Diverse Protein‐Ligand Interactions

Advancements in magnetic nanoparticle-based biosensors for point-of-care testing

Advances in Nanoplasmonic Biosensors: Optimizing Performance for Exosome Detection Applications

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