The Cytobrush Plus cell collector in oral cytology

Jones, A. Vallance; Pink, Frank E.; Sandow, Pamela L.; Stewart, Carol M.; Migliorati, Cesar A.; Baughman, Ron

doi:10.1016/s0030-4220(06)80117-9

Cited by 53 publications

(44 citation statements)

References 2 publications

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“…23 Therefore, the use of brushes to collect epithelial cells allows collection of samples that include cells of all the buccal mucosa stratified squamous epithelium, 9,24,25 in addition to providing a thinner and more dispersed, homogeneous distribution of cells on the slides. 26 In the present study, placement of brackets in the buccal cavity caused diminution of the nucleus, an increase in cytoplasm, and a lower nuclear/cytoplasmic ratio of buccal mucosa cells that were in contact with the brackets. These results corroborate the findings of Shabana et al, 27 who also reported a statistically significant increase in the size of cells of traumatic keratosis lesions when these were compared with normal cells of the buccal mucosa.…”

Section: Discussionsupporting

confidence: 45%

Metal and Ceramic Bracket Effects on Human Buccal Mucosa Epithelial Cells

Pereira

Tanaka

Lima

et al. 2009

The Angle Orthodontist

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Objective: To test the null hypothesis that metal and ceramic brackets have no effect on the epithelial cells of the buccal mucosa. Materials and Methods: Two metal and two ceramic brackets were bonded in 21 individuals of both sexes. With the use of liquid-based exfoliative cytology, morphometric and morphologic changes in buccal mucosa cells adjacent to these brackets were determined and were compared at three time points: baseline (T0), 60 days after placement (T1), and 30 days after removal of the brackets (T2). Results: A decrease in nuclear area and an increase in cytoplasmic area occurred in the buccal mucosa cells adjacent to the brackets at T1 (P Ͻ .01). At T2, this altered morphometry persisted only in cells adjacent to the metal brackets, although to a lesser degree than at T1 (P Ͻ .01). A greater decrease in nuclear area was noted in cells adjacent to the metal brackets than in those next to the ceramic brackets (P Ͻ .01). At T0, the proportions of surface and subsurface cells were similar, but at T1, a predominance of surface cells was observed (P Ͻ .05). At all time points, smears of cells appeared normal or normal with some inflammatory changes. Conclusion:The hypothesis is rejected. Placement of metal and ceramic brackets in the buccal cavity induces cellular alterations. These alterations do not suggest malignancy. (Angle Orthod. 2008;00:373-379.)

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Section: Discussionsupporting

confidence: 45%

Metal and Ceramic Bracket Effects on Human Buccal Mucosa Epithelial Cells

Pereira

Tanaka

Lima

et al. 2009

The Angle Orthodontist

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“…16 A cytobrush, wooden spatula, metal spatula and cotton swab, among others, are the instruments that have been cited in the literature for EC material collection. 5,8,17,18,19 The answer "cotton swab" was considered to be an inappropriate answer because few epithelial cells can be collected with this instrument, possibly due to its non-adhesive surface. 19 The most cited instrument was the wooden spatula; however, using a wooden spatula can result in inadequate material being collected because the wood can absorb part of the sample, thereby reducing the quality of the smear.…”

Section: Discussionmentioning

confidence: 99%

“…5,8,17,18,19 The answer "cotton swab" was considered to be an inappropriate answer because few epithelial cells can be collected with this instrument, possibly due to its non-adhesive surface. 19 The most cited instrument was the wooden spatula; however, using a wooden spatula can result in inadequate material being collected because the wood can absorb part of the sample, thereby reducing the quality of the smear. Some authors have recommended moistening the wooden spatula to prevent dehydration of the site to be sampled.…”

Section: Discussionmentioning

confidence: 99%

“…Some authors have recommended moistening the wooden spatula to prevent dehydration of the site to be sampled. 17 The use of the cytobrush provides more even distribution of cells on the glass slide as compared to the wooden spatula, 19 resulting in a smear with better homogeneity and cellularity than with a metal spatula. 18 Formaldehyde was indicated by 19.9% of the sample as the fixative of choice for EC, which suggests that many dentists could be confusing EC with biopsy.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of dentists’ knowledge of the use of oral exfoliative cytology in clinical practice

et al. 2014

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evaluation of dentists' knowledge of the use of oral exfoliative cytology in clinical practice Abstract: Oral exfoliative cytology (EC) is a rapid and practical complementary test that can be used in the diagnosis of various diseases. The objectives of this study were to assess dentists' knowledge of EC and to disseminate information on the technique. The study included 240 dentists from the city of Anápolis (Goiás, Brazil) who answered a questionnaire regarding demographic data and data relating to EC and who then received information on the technique. The data revealed that 26.92% of the dentists did not know about EC. Among those who reported knowledge of EC, 33.91% did not know the procedures for performing the technique, and 85.38% had no experience with EC. In addition, 54.09% of the answers regarding the instrument used to collect the material and 56.82% of the answers concerning suitable fixatives were inappropriate. Although 73.02% of the dentists adequately identified the pathologies for which EC is recommended, it was concluded that the respondents had poor knowledge of EC. There is a need to increase dentists' awareness of this subject and of EC's use in diagnostic practices.

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“…4 Oral infection by Candida can result in a number of clinical lesions, including median rhomboid glossitis (central papillary atrophy), denture stomatitis, squamous cell carcinoma, Radiation therapy, immunocompromised status (HIV), etc.…”

mentioning

confidence: 99%

Assessment of Candidal carriage in patients with Type II Diabetes Mellitus

et al. 2015

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Background: It is generally acknowledged that patients with diabetes mellitus are more susceptible to fungal infections, particularly with Candida albicans. Oral infection by Candida can result in a number of clinical lesions, including median rhomboid glossitis (central papillary atrophy), denture stomatitis, squamous cell carcinoma, Radiation therapy, immunocompromised status, etc. Different studies have shown that patients with diabetes mellitus have increased frequency of oral candidal carriage and increased risk of candidiasis, which is related to poor metabolic control, neutrophil dysfunction, reduced salivary flow, high glucose concentration in blood and saliva and in medications. Materials and Methods:Subjects of both the groups were given 10 ml of sterile normal saline and asked to rinse the mouth for one minute. The subjects were then asked to return the oral rinse in a sterile clean, broad-mouthed container which was capped, labelled and taken to the laboratory. The samples were then inoculated onto the culture medium (Sabouraud's dextrose agar with Chloramphenicol) with minimal delay (within 6-8 hours of collection of oral rinse). Candidal colonies were counted and compared with non-diabetics.Results: Statistically significant increase in colony forming units (p=0.0324) were obtained in patients with diabetes mellitus. Conclusion:The results indicate significant increase in colonization and carriage of candida in the oral cavity among diabetics when compared with non-diabetics. However, further research using larger samples is required which may lend credibility to the suggestion of increased candidal CFUs in diabetics serving as a surrogate marker of serum glucose levels.

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The Cytobrush Plus cell collector in oral cytology

Cited by 53 publications

References 2 publications

Metal and Ceramic Bracket Effects on Human Buccal Mucosa Epithelial Cells

Metal and Ceramic Bracket Effects on Human Buccal Mucosa Epithelial Cells

Evaluation of dentists’ knowledge of the use of oral exfoliative cytology in clinical practice

Assessment of Candidal carriage in patients with Type II Diabetes Mellitus

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