The DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosis

Abstract

Background Deciphering the mechanisms of meiosis has important implications for potential applications in plant breeding programmes and species evolution. However, the process of meiosis is poorly understood in carnation, which is famous for its cut flowers. Results We report that Dianthus caryophyllus parallel spindle 1 (DcPS1) regulates omission of second division like a (OSDLa) during pollen development and 2n gamete production in carnation meiosis. In DcPS1 and OSDLa RNAi lines, an absence of the second meiotic division and the abnormal orientation of spindles at meiosis II might be the main reason for dyad/triad formation, resulting in unreduced gametes. We also found that carnation OSDLa interacted with DcPS1 and DcRAD51D. In the DcPS1 RNAi lines, a decrease in OSDLa and DcRAD51D expression was observed. In the OSDLa RNAi lines, a decrease in DcPS1 and DcRAD51D expression was also observed. We propose that DcPS1 regulates OSDLa expression, allowing entry into meiosis II and the proper orientation of the metaphase II spindle in meiosis II. We also propose that OSDLa regulates DcRAD51D expression, allowing for homologous recombination. Conclusions These results suggest a critical role for DcPS1 and OSDLa in diplogamete production during meiosis and open a new pathway for meiosis-related studies.

“Omic tools” for investigation creative plant systens

“…Among the former plants tolerant and sensitive objectes are tested. For example: to reveal the molecular basis of differential reactions to salt stress in two contrast bean genotypes comparative transcriptome, metabplpme, and ionome there are developed [150,151,152]. Transcritomics demonstrated active carbon and amino acid metabolism for the tolerant genotype.…”

“…Multi-omics investigation is developed with the addition of agricultural plants [151,154]. Wheat (Triticum aestivum L.) can accumulate high contents of heavy metal ions in edible parts.…”

“…Wheat (Triticum aestivum L.) can accumulate high contents of heavy metal ions in edible parts. Understanding of wheat response to heavy metal stress and its management in decreasing ions uptake and accumulation may help to improve its growth and grain quality [151]. Rice is one of the most important food crops, feeding more than half of the world"s population.…”

Comparative transcriptome analysis provides insights into molecular pathway and genes associated with head-type formation and phenotypic divergence in Chinese cabbage

“…2n gametes can be used in plant polyploid breeding. At present, 2n gametes have been extensively studied, such as in Chinese cabbage ( Zhang et al, 2009 ), potato ( Peloquin et al, 2008 ), kiwi ( Seal et al, 2012 ), sorghum ( Hodnett et al, 2019 ), Arabidopsis ( Yi et al, 2023 ), eggplant ( Carputo, 2003 ), and carnation ( Zhou et al, 2015 ; Zhou et al, 2022 ). 2n gametes can be utilized to produce new polyploid resources.…”