The defence elicitor AsES causes a rapid and transient membrane depolarization, a triphasic oxidative burst and the accumulation of nitric oxide

Martos, Gustavo Gabriel; Teran, María Del Milagro; Ricci, Juan C. Díaz

doi:10.1016/j.plaphy.2015.10.029

Cited by 9 publications

(6 citation statements)

References 49 publications

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“…Plants stimulated by protein elicitors will produce a series of defense responses (e.g., oxidative burst, HR, and nitric oxide accumulation) (Gabriel et al 2015 ; Foissner 2000 ), which subsequently activate the disease resistance-related metabolic activities involving the JA, ethylene, and aABA signaling pathways. Moreover, there is “cross-talk” among the various signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice

Yan

Zheng

et al. 2021

Rice

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Protein elicitors can induce plant systemic resistance to pathogens. In an earlier study, we cloned an EsxA gene from the plant growth-promoting rhizobacterium Paenibacillus terrae NK3-4 and expressed it in Pichia pastoris. In addition to being important for the pathogenicity of animal pathogens, EsxA can also induce an immune response in animals. While, we found the exogenously expressed EsxA has the activity of elicitor, which can trigger hypersensitive response and reactive oxygen species burst in leaves as well as enhanced rice plant growth. The effects of EsxA on seedling blight (Fusarium oxysporum) resistance and gene transcription, including pathogenesis-related (PR) genes in rice were evaluated. The germination rate was 95.0% for seeds treated with EsxA and then inoculated with F. oxysporum, which was 2.8-times higher than that of F. oxysporum-infected control seeds that were not treated with EsxA (Con). The buds and roots of EsxA-treated seedlings were 2.4- and 15.9-times longer than those of Con seedlings. The plants and roots of seedlings dipped in an EsxA solution and then inoculated with F. oxysporum were longer than those of the Con seedlings. Theplant length, number of total roots, and number of white roots were respectively 23.2%, 1.74-times, and 7.42-times greater for the seedlings sprayed with EsxA and then inoculated with F. oxysporum than for the Con seedlings. The EsxA induction efficiency (spray treatment) on seedling blight resistance was 60.9%. The transcriptome analysis revealed 1137 and 239 rice genes with EsxA-induced up-regulated and down-regulated transcription levels, respectively. At 48 h after the EsxA treatment, the transcription of 611 and 160 genes was up-regulated and down-regulated, respectively, compared with the transcription levels for the untreated control at the same time-point. Many disease resistance-related PR genes had up-regulated transcription levels. The qPCR data were consistent with the transcriptome sequencing results. EsxA triggered rice ISR to seedling blight and gene differential transcription, including the up-regulated transcription of rice PR genes. These findings may be relevant for the use of EsxA as a protein elicitor to control plant diseases.

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Section: Discussionmentioning

confidence: 99%

Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice

Yan

Zheng

et al. 2021

Rice

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“…For example, redox homeostasis is necessary for symbiosis between legumes and rhizobia, inoculation of M. truncatula seedlings with pathogenic or symbiotic bacteria induces the oxidative burst in the host, with a major difference in the levels of ROS production ( Peleg-Grossman et al, 2012 ). Strawberry plant cells treated with AsES elicitor obtained from Acremonium strictum exhibited a triphasic production of H 2 O 2 and a rapid intracellular accumulation of NO ( Martos et al, 2015 ).…”

Section: Hormetic Dose–response In Plantsmentioning

confidence: 99%

Plant Hormesis Management with Biostimulants of Biotic Origin in Agriculture

et al. 2017

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Over time plants developed complex mechanisms in order to adapt themselves to the environment. Plant innate immunity is one of the most important mechanisms for the environmental adaptation. A myriad of secondary metabolites with nutraceutical features are produced by the plant immune system in order to get adaptation to new environments that provoke stress (stressors). Hormesis is a phenomenon by which a stressor (i.e., toxins, herbicides, etc.) stimulates the cellular stress response, including secondary metabolites production, in order to help organisms to establish adaptive responses. Hormetins of biotic origin (i.e., biostimulants or biological control compounds), in certain doses might enhance plant performance, however, in excessive doses they are commonly deleterious. Biostimulants or biological control compounds of biotic origin are called “elicitors” that have widely been studied as inducers of plant tolerance to biotic and abiotic stresses. The plant response toward elicitors is reminiscent of hormetic responses toward toxins in several organisms. Thus, controlled management of hormetic responses in plants using these types of compounds is expected to be an important tool to increase nutraceutical quality of plant food and trying to minimize negative effects on yields. The aim of this review is to analyze the potential for agriculture that the use of biostimulants and biological control compounds of biotic origin could have in the management of the plant hormesis. The use of homolog DNA as biostimulant or biological control compound in crop production is also discussed.

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“…For leaf disc experiments, 5-mm diameter discs were obtained by punching leaflets of 8-week-old plants. Cell extraction was performed according to Martos et al (2015) and cells were suspended in W5 modified solution (154 mM NaCl, 5 mM KCl, 125 mM CaCl 2 , and 0.5 M sucrose, 2 mM, MES, pH 5.6) at 10 6 cells mL -1 .…”

Section: Plants and Growth Conditionsmentioning

confidence: 99%

“…The accumulation of H 2 O 2 was evaluated using the fluorescent probe H2DCF-DA (SigmaeAldrich) (Li et al, 2012;Ye et al, 2013) according to Martos et al (2015). We used a pulse and chase approach to evaluate the H 2 O 2 production at different times after HeT treatment.…”

Section: Intracellular H 2 O 2 Determinationmentioning

confidence: 99%

The ellagitannin HeT induces electrolyte leakage, calcium influx and the accumulation of nitric oxide and hydrogen peroxide in strawberry

Martos

Mamani

Filippone

et al. 2018

Plant Physiology and Biochemistry

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The defence elicitor AsES causes a rapid and transient membrane depolarization, a triphasic oxidative burst and the accumulation of nitric oxide

Cited by 9 publications

References 49 publications

Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice

Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice

Plant Hormesis Management with Biostimulants of Biotic Origin in Agriculture

The ellagitannin HeT induces electrolyte leakage, calcium influx and the accumulation of nitric oxide and hydrogen peroxide in strawberry

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