1992
DOI: 10.1007/bf00192794
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The defense-related STH-2 gene product of potato shows race-specific accumulation after inoculation with low concentrations of Phytophthora infestans zoospores

Abstract: The defense-related STH-2 gene is rapidly activated following infection or elicitor treatment of potato (Solanum tuberosum L.) tubers. However, its physiological or biochemical function is unknown. To study the STH-2 gene product and its accumulation during the defense response, we raised antibodies to a β-galactosidase-STH-2 fusion protein in Escherichia coli. The antiserum specifically recognized a protein of the predicted 17-kDa size in extracts of elicited tuber disks when analyzed by Western blot. In cont… Show more

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Cited by 35 publications
(32 citation statements)
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“…For protein gel blots, DNA affinity-purified proteins from 250 g of potato tubers were precipitated with 5 volumes of cold acetone, resuspended in SDS sample buffer, and separated by SDS-PAGE. Protein gel blots were performed as described previously (Constable and Brisson, 1992) with p24 antibody used at 1000-fold dilution. The blots were developed with the electrochemiluminescent detection system (Amersham Pharmacia Biotech) according to the manufacturer's instructions.…”
Section: Protein Gel Blot Of Pbf-2mentioning
confidence: 99%
“…For protein gel blots, DNA affinity-purified proteins from 250 g of potato tubers were precipitated with 5 volumes of cold acetone, resuspended in SDS sample buffer, and separated by SDS-PAGE. Protein gel blots were performed as described previously (Constable and Brisson, 1992) with p24 antibody used at 1000-fold dilution. The blots were developed with the electrochemiluminescent detection system (Amersham Pharmacia Biotech) according to the manufacturer's instructions.…”
Section: Protein Gel Blot Of Pbf-2mentioning
confidence: 99%
“…Total proteins were extracted from fresh and treated discs, separated on 14% (for pathogenesis-related protein PR-1 Oa), 10% (for PKC), and 12% (for chitinase and glucanase) SDS-polyacrylamide gels, and blotted onto nitrocellulose membranes as previously described (Constabel and Brisson, 1992). The blots were stained with Ponceau S, blocked, and incubated with anti-PR-1 Oa antibodies, as described previously (Constabel and Brisson, 1992). The anti-PKC antibody was used at 100 ng/mL.…”
Section: Treatments Of Tuber Discs For Lmmunoblot Analysesmentioning
confidence: 99%
“…Elicitor treatment and inoculation of the treated tubers with Phytophthora infestans (race 1,2,3,4) were as described before (Constabel and Brisson, 1992). The tissue was incubated on moist filter paper in Petri dishes and incubated in the dark at 15°C.…”
Section: Pathogen Lnfection and Ellsasmentioning
confidence: 99%
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“…Because some defense reactions have been reported to be induced more rapidly after inoculation in an incompatible than in a compatible interaction, 18,19) the course of phyotocas- sane accumulation in rice plants was examined in both types of interaction after inoculation. When theˆfth leaf of rice plants (Oryzae sativa, L cv.…”
mentioning
confidence: 99%