The defensive effect of phellodendrine against AAPH-induced oxidative stress through regulating the AKT/NF-κB pathway in zebrafish embryos

Li, Ling; Huang, Tao; Tian, Chen; Xiao, Yali; Kou, Shuming; Zhou, Xia; Liu, Shanshan; Yang, Xiaoli; Li, Xuegang

doi:10.1016/j.lfs.2016.05.032

Cited by 38 publications

(24 citation statements)

References 34 publications

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“…65 Phellodendrine (198) was identified from Phellodendri chinensis cortex. 77 In 2017, a new glycoalkaloid, (−)-1-Oβ-D-glucoside-8-oxotetrahydropalmatine (199), isolated from tubers of S. succifera, exhibited antimicrobial activity against Staphylococcus aureus. 57 N-Methylcanadine (200) was isolated from Zanthoxylum tingoassuiba, 78 and demethylalangiside (201), alangiside (202), and isoalangiside (203) were identified and isolated from Ophiorrhiza nutans.…”

Section: Protoberberine Isoquinoline Alkaloidsmentioning

confidence: 99%

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Biologically active isoquinoline alkaloids covering 2014–2018

Shang

Yang

Morris‐Natschke

et al. 2020

Medicinal Research Reviews

147

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Isoquinoline alkaloids, an important class of N-based heterocyclic compounds, have attracted considerable attention from researchers worldwide since the early 19th century. Over the past 200 years, many compounds from this class were isolated, and most of them and their analogs possess various bioactivities. In this review, we survey the updated literature on bioactive alkaloids and highlight research achievements of this alkaloid class during the period of 2014-2018. We reviewed over 400 molecules with a broad range of bioactivities, including antitumor, antidiabetic and its complications, antibacterial, antifungal, antiviral, antiparasitic, insecticidal, anti-inflammatory, antioxidant, neuroprotective, and other activities. This review should provide new indications or directions for the discovery of new and better drugs from the original naturally occurring isoquinoline alkaloids.

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Section: Protoberberine Isoquinoline Alkaloidsmentioning

confidence: 99%

“…In vitro, the compound decreased the increased ROS production, lipid-peroxidation, and cell death rate caused by AAPH-induced oxidative stress, most likely by downregulation of AKT phosphorylation and NF-κB3 expression. 77 Oleracein E (403) has a rare tetrahydroisoquinoline/pyrrolidone tricyclic skeleton and a catechol moiety. 300…”

Section: Antioxidant Activitymentioning

confidence: 99%

Biologically active isoquinoline alkaloids covering 2014–2018

Shang

Yang

Morris‐Natschke

et al. 2020

Medicinal Research Reviews

147

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“…As a result, the contents of phellodendrine and berberine were determined to be 22.255 ± 0.123 mg/g and 269.651 ± 1.257 mg/g, respectively. Phellodendrine has been reported to have antioxidant and anti-inflammatory properties [ 16 ], and to act as an immunosuppressor [ 17 , 18 ]. Many studies have reported the biological and clinical efficacies of berberine from several different herbal plants, such as Berberis vulgaris , Hydrastis canadensis , Xanthorhiza simplicissima , P. amurense , and Coptis chinensis .…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Quantification and the In Vitro Acetylcholinesterase Inhibitory Activity of Phellodendron chinense and Its Components

et al. 2017

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The dried bark of Phellodendron chinense has been used as a traditional herbal medicine to remove damp heat, relieve consumptive fever, and cure dysentery and diarrhea. In the present study, we performed quantitative analyses of the two components of P. chinense, phellodendrine and berberine, using high-performance liquid chromatography. A 70% ethanol extract of P. chinense was prepared and the two components were separated on a C-18 analytical column using a gradient solvent system of acetonitrile and 0.1% (v/v) aqueous trifluoroacetic acid. The ultraviolet wavelength used for detection was 200 nm for phellodendrine and 226 nm for berberine. The analytical method established here showed high linearity (correlation coefficient, ≥0.9991). The amount of phellodendrine and berberine used was 22.255 ± 0.123 mg/g and 269.651 ± 1.257 mg/g, respectively. Moreover, we performed an in vitro acetylcholinesterase (AChE) activity assay and an amyloid-β aggregation test to examine the biological properties of phellodendrine and berberine as therapeutic drugs for Alzheimer’s disease. Phellodendrine and berberine inhibited AChE activity in a dose-dependent manner (IC50 = 36.51 and 0.44 μM, respectively). In contrast, neither phellodendrine nor berberine had an effect on amyloid-β aggregation. The P. chinense extract and phellodendrine, but not berberine, exhibited antioxidant activity by increasing radical scavenging activity. Moreover, P. chinense demonstrated a neuroprotective effect in hydrogen peroxide-treated HT22 hippocampal cells. Overall, our findings suggest that P. chinense has potential as an anti-Alzheimer’s agent via the suppression of the enzymatic activity of acetylcholinesterase and the stimulation of antioxidant activity.

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“…(2.5 μ M) which targets on NF-κB, AKT, and ERK pathways, respectively. The concentration of inhibitors were determined by LC50 values [20] . The 48 hpf embryos were pre-treated with inhibitors for 2 hr, followed by treatment with DOX, VLB, GEF, GEF+DOX, and GEF+VLB until 120 hpf, respectively.…”

Section: Treatment In Zebrafish Embryosmentioning

confidence: 99%

An engineered Abcb4 expressing model reveals the central role of NF-κB in the regulation of drug resistance in zebrafish

Sun

et al. 2020

Preprint

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Multidrug resistance (MDR) represents the major cause of unsatisfaction in the application of chemotherapy for cancer treatment. So far, an in vivo robust high-throughput screening system for anti-tumor drug MDR is still lacking and the molecular mechanisms for MDR still remain elusive. Given a myriad of merits of zebrafish relative to other animal models, we aimed to establish MDR system in zebrafish stably expressing ATP-binding cassette (ATP-cassette) superfamily transporters and study the potential regulatory mechanism. We first constructed a Tg(abcb4:EGFP) transgenic zebrafish stably expressing both Abcb4 and EGFP using Tol2mediated approach. The expression level of Abcb4 and EGFP was significantly induced when Tg(abcb4:EGFP) transgenic zebrafish embryos were exposed to doxorubicin (DOX) or vincristine (VCR), accompany with a marked decrease in rhodamine B (RhB) accumulation in embryos, which indicates a remarkable increase in drug efflux upon the exposure to DOX or VCR. Mechanistically, AKT and ERK signaling were activated when treated with DOX or VCR.With the application of AKT and ERK inhibitors, the drug resistance phenomena could be reversed with differential responsive effects. Of note, downstream NF-κB played a central role in the regulation of Abcb4-mediated drug resistance. Taken together, the engineered Tg(abcb4:EGFP) transgenic zebrafish model provides a new platform for drug resistance screening in vivo, which could facilitate and accelerate the process of drug development.

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The defensive effect of phellodendrine against AAPH-induced oxidative stress through regulating the AKT/NF-κB pathway in zebrafish embryos

Cited by 38 publications

References 34 publications

Biologically active isoquinoline alkaloids covering 2014–2018

Biologically active isoquinoline alkaloids covering 2014–2018

Phytochemical Quantification and the In Vitro Acetylcholinesterase Inhibitory Activity of Phellodendron chinense and Its Components

An engineered Abcb4 expressing model reveals the central role of NF-κB in the regulation of drug resistance in zebrafish

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