The dengue viruses

Henchal, Erik A.; Putnak, J. Robert

doi:10.1128/cmr.3.4.376

Cited by 533 publications

(413 citation statements)

References 256 publications

Supporting

Mentioning

386

Contrasting

Unclassified

Order By: Relevance

“…This disease most often occurs during the rainy season and may cause various clinical manifestations that range from asymptomatic infections to more severe conditions. The initial phase of dengue is generally characterized by an acute febrile syndrome (fever, adynamia, headache, retro-orbital pain, myalgia and arthralgia), without symptoms or focal signs (1)(2)(3).…”

mentioning

confidence: 99%

See 1 more Smart Citation

La evidencia serológica de leptospirosis en pacientes con sospecha clínica de dengue en el estado de Ceará, Brasil

et al. 2015

View full text Add to dashboard Cite

mentioning

confidence: 99%

“…Specific laboratory techniques are therefore needed for diagnosis. In dengue-endemic regions, it is essential to perform a differential diagnosis to differentiate it from other febrile syndromes such as leptospirosis (1,6).…”

mentioning

confidence: 99%

La evidencia serológica de leptospirosis en pacientes con sospecha clínica de dengue en el estado de Ceará, Brasil

et al. 2015

View full text Add to dashboard Cite

“…1,2 The global prevalence of dengue has grown dramatically in recent decades. The disease is now endemic in more than 100 countries.…”

mentioning

confidence: 99%

Liver injury caused by antibodies against dengue virus nonstructural protein 1 in a murine model

Lin

Wan

Chen

et al. 2008

Laboratory Investigation

View full text Add to dashboard Cite

Clinical manifestations of severe dengue diseases include thrombocytopenia, vascular leakage, and liver damage. Evidence shows that hepatic injury is involved in the pathogenesis of dengue infection; however, the mechanisms are not fully resolved. Our previous in vitro studies suggested a mechanism of molecular mimicry in which antibodies directed against dengue virus (DV) nonstructural protein 1 (NS1) cross-reacted with endothelial cells and caused inflammatory activation and apoptosis. In this study, the pathogenic effects of anti-DV NS1 antibodies were further examined in a murine model. We found, in liver sections, that anti-DV NS1 antibodies bound to naive mouse vessel endothelium and the binding activity was inhibited by preabsorption of antibodies with DV NS1. Active immunization with DV NS1 resulted in antibody deposition to liver vessel endothelium, and also apoptotic cell death of liver endothelium. Liver tissue damage was observed in DV NS1-immunized mice by histological examination. The serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were increased in mice either actively immunized with DV NS1 protein or passively immunized with antibodies obtained from DV NS1-immunized mice. Furthermore, histological examination revealed mononuclear phagocyte infiltration and cell apoptosis in mice passively immunized with antibodies obtained from mice immunized with DV NS1. Increased AST and ALT levels were observed in mice passively immunized with purified immunoglobulin G (IgG) from dengue patients compared with normal control human IgG-immunized mice. The increased AST and ALT levels were inhibited when dengue patient serum IgG was preabsorbed with DV NS1. In conclusion, active immunization with DV NS1 protein causes immune-mediated liver injury in mice. Passive immunization provides additional evidence that anti-DV NS1 antibodies may play a role in liver damage, which is a pathologic manifestation in dengue virus disease.

show abstract

“…

…”

mentioning

confidence: 99%

Histopathological and ultrastructural aspects of mice lungs experimentally infected with dengue virus serotype 2

Barreto¹,

Takiya²,

Schatzmayr³

et al. 2007

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Key words: dengue-2 virus -BALB/c mice -C6/36 cell line -macrophage -mast cell -ultrastructure Dengue fever (DF) is an acute infectious disease caused by dengue virus (DENV) (Halstead 1988, Henchal & Putnak 1990) that belong to the Flavivirus genus of the Flaviviridae family of single-stranded, positive-polarity, enveloped RNAviruses (Chambers et al. 1990). Dengue disease (DEN) has a spectrum of clinical signs and symptoms, ranging from asymptomatic infection to severe and lethal manifestations. The four DENV serotypes (DENV-1, -2, -3, -4) may cause dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) in humans, with an estimated 100 million new cases of DF and over 250,000 cases of DHF/DSS per year worldwide. The major difficulty in studying DENV infection in humans and for developing a vaccine, is the absence of a suitable animal model which develops a disease with similar aspects of the DHF and DSS (Bhamarapravati 1993). Several studies suggest that mice are a permissive host for DENV (Meiklejhon et al. 1952, Lin et al.1998, Johnson & Roehrig 1999), but in the majority of these models the animals are im- (Table). In the present study we characterized the injuries caused by DENV-2 infection in lung tissue of BALB/c mice using photonic and electron transmission microscopies. Differing from the majority of the mice models, the DENV-2 (nonneuroadapted) were obtained from a patient serum, propagated once in the C6/36 cell line and inoculated by intravenous (i.v.) and intraperitoneal (i.p.) routes. MATERIALS AND METHODS Virus -The virus strain used in our experiments was isolated from a patient serum during an epidemic of DENV-2 in the state of Rio de Janeiro in 1995 and propagated in the Aedes albopictus mosquito cell line (C6/ 36). The serum was tested by the indirect immunofluorescence technique using a type specific DENV-2 monoclonal (3H5, DENV-2) antibody. The virus had not undergone passage in mouse brain. The titer of the virus (10 6.3 TCID 50 /0.1 ml) was calculated by the method of Reed and Muench (1938).Cells -The C6/36 monolayers were grown in L-15 medium supplemented with 1% non-essencial aminoacids, 10% tryptose phosphate broth, and 10% fetal bovine serum. The tubes were kept at 28 o C.Animal -Adult male BALB/c mice, aged 2 months and weighing 25 g, were obtained from the mouse colony maintained in the Department of Virology of the Instituto Oswaldo Cruz-Fiocruz. Mice were i.p. and i.v. inoculated with DENV-2 (dose of 10,000 TCID 50 /0.2 ml) and sacrificed after 48, 72 h, 7 and 49 days p.i. Non-infected mice and mice inoculated with L-15 medium were used as controls and sacrificed at the same time. The experiments were previously approved by the Animal Experimentation Ethical Committee of the Instituto Oswaldo Cruz-Fiocruz.Processing of tissues for photonic microscopy analysis -The animals were peritoneally anaesthetized and lung tissue fragments were collected from infected and non-infected mice. Samples were fixed in Millonig's fixative, dehydrated in ethanol, and paraffin-embedded. Sections (5...

show abstract

The dengue viruses

Cited by 533 publications

References 256 publications

La evidencia serológica de leptospirosis en pacientes con sospecha clínica de dengue en el estado de Ceará, Brasil

La evidencia serológica de leptospirosis en pacientes con sospecha clínica de dengue en el estado de Ceará, Brasil

Liver injury caused by antibodies against dengue virus nonstructural protein 1 in a murine model

Histopathological and ultrastructural aspects of mice lungs experimentally infected with dengue virus serotype 2

Contact Info

Product

Resources

About