The dense granule protein GRA2, a new marker for the serodiagnosis of acute Toxoplasma infection: comparison of sera collected in both France and Iran from pregnant women

Golkar, Majid; Rafati, Sima; Abdel-Latif, Mohamed S.; Brenier-Pinchart, Marie-Pierre; Fricker‐Hidalgo, Hélène; Khoshkholgh-Sima, Baharak; Babaie, Jalal; Pelloux, Hervé; Cesbron-Delauw, Marie-France; Mercier, Corinne

doi:10.1016/j.diagmicrobio.2007.03.003

Cited by 43 publications

(24 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This expression system and the method of purification by a one-step metal affinity chromatography led to the production of milligram amounts of pure recombinant proteins (28 mg of r-GRA2 and 16 mg of r-ROP1) per liter of culture. The production efficiency of this system is much higher than that which produced the thioredoxin-His fusion GRA2 and rGRA2 proteins obtained by others (10,11).…”

Section: Discussionmentioning

confidence: 68%

“…Furthermore, immunization with purified GRA2 has been shown to induce both a vigorous antibody response and a T-cell response (11,20,25). In addition, GRA2 and ROP1 allowed the detection of T. gondii-specific IgG in humans with toxoplasmosis (2,10,23). Taken together, these data suggest that the GRA2 and ROP1 antigens represent promising diagnostic tools for detection of toxoplasmosis in humans.…”

mentioning

confidence: 87%

“…Dense granule antigens (GRA) are major components of both the vacuole surrounding tachyzoites and the cyst wall surrounding slower-growing bradyzoites (6), making GRA and ROP promising diagnostic tools and important protective antigens. At present, many investigators are studying the serological applications and the protective immunity induced by secretory organelles (9,10,15).…”

mentioning

confidence: 99%

See 2 more Smart Citations

GRA2 and ROP1 Recombinant Antigens as Potential Markers for Detection of Toxoplasma gondii -Specific Immunoglobulin G in Humans with Acute Toxoplasmosis

Holec-Gąsior

Kur

Hiszczyńska-Sawicka

2009

Clin Vaccine Immunol

View full text Add to dashboard Cite

A goal of the current study was to evaluate serological applications of Toxoplasma gondii GRA2 and rhoptry protein 1 (ROP1) antigens. Soluble recombinant GRA2 and ROP1 antigens as fusion proteins containing six histidyl residues at the N and C terminals were obtained using an Escherichia coli expression system. Purification by one-step metal affinity chromatography allowed recovery of milligram amounts of pure recombinant proteins per liter of culture. The usefulness of these antigens for diagnosis of human infections was tested on 167 serum samples obtained during routine diagnostic tests. A panel of 37 serum samples from patients with acute toxoplasmosis was compared to a panel of 90 serum samples from individuals with past infection. The results indicated that both GRA2 and ROP1 recombinant antigens detected antibodies more frequently in samples from individuals with acute infections (100% and 94.6%, respectively) than in samples from individuals with chronic infections (22.5% and 15.5%, respectively). These results suggest that immunoglobulin G antibodies against GRA2 and ROP1 antigens are produced during the acute stage of toxoplasmosis but are uncommon in the chronic phase of the infection. Hence, these recombinant proteins can be used as specific molecular markers to differentiate between acute and chronic infections.

show abstract

Section: Discussionmentioning

confidence: 68%

mentioning

confidence: 87%

mentioning

confidence: 99%

See 1 more Smart Citation

GRA2 and ROP1 Recombinant Antigens as Potential Markers for Detection of Toxoplasma gondii -Specific Immunoglobulin G in Humans with Acute Toxoplasmosis

Holec-Gąsior

Kur

Hiszczyńska-Sawicka

2009

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…greater levels of anti-GRA2 IgG antibodies were detected in sera from pregnant women with acute infection than in sera from those with chronic infection (14). Therefore, it appears that production of antibodies to GRA2 and ROP1 is associated with proliferation of tachyzoites not only in the brain but also in other organs.…”

Section: Discussionmentioning

confidence: 94%

Toxoplasma gondii Antigens Recognized by IgG Antibodies Differ between Mice with and without Active Proliferation of Tachyzoites in the Brain during the Chronic Stage of Infection

Hester

Mullins

et al. 2012

Infect Immun

Self Cite

View full text Add to dashboard Cite

We examined whether tachyzoite proliferation in the brains of immunocompetent hosts during the chronic stage of infection with Toxoplasma gondii induces production of IgG antibodies that recognize parasite antigens different from those recognized by the antibodies of infected hosts that do not have tachyzoite growth. For this purpose, two groups of CBA/J mice, which display continuous tachyzoite growth in their brains during the later stage of infection, were infected, and one group received treatment with sulfadiazine to prevent tachyzoite proliferation during the chronic stage of infection. T. gondii antigens recognized by the IgG antibodies from these two groups of mice were compared using immunoblotting following separation of tachyzoite antigens by two-dimensional gel electrophoresis. Several antigens, including the microneme protein MIC2, the cyst matrix protein MAG1, and the dense granule proteins GRA4 and GRA7, were commonly recognized by IgG antibodies from both groups of mice. There were multiple antigens recognized mostly by IgG antibodies of only one group of mice, either with or without cerebral tachyzoite growth. The antigens recognized only by or mostly by the antibodies of mice with cerebral tachyzoite growth include MIC6, the rhoptry protein ROP1, GRA2, one heat shock protein HSP90, one (putative) HSP70, and the myosin heavy chain. These results indicate that levels of IgG antibody to only selected T. gondii antigens increase in association with cerebral tachyzoite proliferation (reactivation of infection) in immunocompetent hosts with chronic infection.

show abstract

“…Upgrading these tests with recombinant proteins will improve their sensitivity and specificity which will have major advantages for the diagnosis of toxoplasmosis and this will reduce the problems of antigen standardization and assay reproducibility which is a major problem of diagnostic laboratories worldwide [6]. The surface antigens SAG1 (p30) [7][8][9][10][11], SAG2 (P22) [7,9,10]; the dense granule antigens GRA1 (P24) [12][13][14][15], GRA2 (P28) [16,17], GRA6 (P32) [14,18,19] and GRA7 (P29) [9,10,15,20,21] the rhoptry antigens ROP1 (P66) [17,22,23]; the microneme antigens MAG1 [24,25] and MIC1 [24,26] bodies in humans and animals. Toxoplasma gondii Subtilisin-like protein 1 (TgSUB1) have been suggested to play an important role in survival of parasite.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Sequencing of Truncated Toxoplasma gondii Subtilisin-Like 1 Antigen

Rouhizadeh

Ghadiri

Jalali

et al. 2016

Zahedan J Res Med Sci

View full text Add to dashboard Cite

Background: Toxoplasma gondii is an obligate intracellular protozoan parasite which has significant medical and veterinary impact on all around the world. Tracking of specific antigens or antibodies for toxoplasmosis is the main choice in its diagnosis. Recombinant proteins will improve sensitivity and specificity and reduce problems of standardization and reproducibility of diagnostic kits. Toxoplasma gondii Subtilisin-like protein (TgSUB1) is a novel example of a glycosylphosphatidylinositol) GPI (-anchored protein which can be considered as a potential marker for serodiagnosis of toxoplasmosis.

show abstract

The dense granule protein GRA2, a new marker for the serodiagnosis of acute Toxoplasma infection: comparison of sera collected in both France and Iran from pregnant women

Cited by 43 publications

References 24 publications

GRA2 and ROP1 Recombinant Antigens as Potential Markers for Detection of Toxoplasma gondii -Specific Immunoglobulin G in Humans with Acute Toxoplasmosis

GRA2 and ROP1 Recombinant Antigens as Potential Markers for Detection of Toxoplasma gondii -Specific Immunoglobulin G in Humans with Acute Toxoplasmosis

Toxoplasma gondii Antigens Recognized by IgG Antibodies Differ between Mice with and without Active Proliferation of Tachyzoites in the Brain during the Chronic Stage of Infection

Cloning and Sequencing of Truncated Toxoplasma gondii Subtilisin-Like 1 Antigen

Contact Info

Product

Resources

About