The desaturation and elongation of linolenic acid and eicosapentaenoic acid by hepatocytes and liver microsomes from rainbow trout (Oncorhynchus mykiss) fed diets containing fish oil or olive oil

Buzzi, Marco; Henderson, R. J.; Sargent, J. R.

doi:10.1016/0005-2760(95)00211-1

Cited by 164 publications

(140 citation statements)

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“…Interestingly, comparison of the data from previous studies on salmonids showed that the apparent rank order for the desaturation of 18:3n-3 to HUFA in the different species fed diets containing fish oil was rainbow trout > Atlantic salmon ≥ Arctic charr (Olsen and Ringo, 1992;Buzzi et al, 1996;Bell et al, 1997;Tocher et al, 2000). These earlier studies had many methodological differences with the Arctic charr data being obtained from in vivo 14 C-fatty acid injection studies (Olsen and Ringo, 1992), whereas the rainbow trout and salmon studies both used isolated hepatocytes.…”

Section: Discussionmentioning

confidence: 96%

“…These earlier studies had many methodological differences with the Arctic charr data being obtained from in vivo 14 C-fatty acid injection studies (Olsen and Ringo, 1992), whereas the rainbow trout and salmon studies both used isolated hepatocytes. However, the methods for isolating the cells varied between these hepatocyte studies and the trout were maintained in indoor aquaria under controlled photoperiod (Buzzi et al, 1996) whereas the salmon trials were performed under natural photoperiod in outdoor tanks Tocher et al, 2000). Diets and feeding regimes also varied in all the previous studies.…”

Section: Discussionmentioning

confidence: 99%

“…In a similar recent study of salmon during smoltification, the peak activity of hepatocyte fatty acyl desaturation of [1-14 C]18:3n-3 was 2.4-fold and 1.4-fold greater in fish fed diets containing rapeseed oil or linseed oil, respectively, compared to a diet containing fish oil (Tocher et al, 2000). Buzzi et al (1996) showed that in rainbow trout fed an olive oil diet (containing 18:2n-6), the recovery of radiolabelled DHA in polar lipids from hepatocytes incubated with [1-14 C]18:3n-3 was double that of fish fed a diet containing fish oil. The recovery of desaturated products of intraperitoneally injected [1- 14 C]18:3n-3 and [1-14 C]18:2n-6 from Arctic charr was increased considerably in fish fed diets containing 1% 18:2n-6 or 1% 18:3n-3 compared to fish fed a commercial, fish oil-containing diet (Olsen and Ringo, 1992).…”

Section: Discussionmentioning

confidence: 99%

“…Desaturase activities in salmon, previously fed vegetable oil during the parr stage were reduced to very low levels upon feeding a diet containing fish oil simultaneous with transferring the smolts to seawater Tocher et al, 2000). Increased hepatic fatty acid desaturase activities were also induced in rainbow trout fed a diet containing olive oil compared to a diet containing fish oil (Buzzi et al, 1996). The conversion of intraperitoneally injected 14 C-labelled 18:2n-6 and 18:3n-3 to HUFA was increased in Arctic charr (Salvelinus alpinus) fed diets containing only C 18 PUFA compared to commercial diets containing fish oil (Olsen and Ringo, 1992).…”

Section: Introductionmentioning

confidence: 96%

“…The conversion of intraperitoneally injected 14 C-labelled 18:2n-6 and 18:3n-3 to HUFA was increased in Arctic charr (Salvelinus alpinus) fed diets containing only C 18 PUFA compared to commercial diets containing fish oil (Olsen and Ringo, 1992). Moreover, comparison of these studies on various salmonids showed that the extent of their ability to desaturate C 18 PUFA appeared to vary between the species (Olsen and Ringo, 1992;Buzzi et al, 1996;Bell et al, 1997;Tocher et al, 2000). Furthermore, in the course of work investigating smoltification in different populations of Atlantic salmon (wild, Scottish farmed stock and Norwegian farmed stock), we have also observed variation in hepatic fatty acid desaturation/elongation activities between the different populations (Tocher, D.R.…”

Section: Introductionmentioning

confidence: 99%

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Hepatocyte fatty acid desaturation and polyunsaturated fatty acid composition of liver in salmonids: effects of dietary vegetable oil

Tocher

Bell

MacGlaughlin

et al. 2001

Comparative Biochemistry and Physiology Part B: Biochemistry an

138

106

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Abbreviations: AA, arachidonic acid; BHT, butylated hydroxytoluene; DHA, docosahexaenoic acid; EFA, essential fatty acid; EPA, eicosapentaenoic acid; FAF-BSA, fatty acid free bovine serum albumin; FO, fish oil; HBSS, Hanks balanced salt solution; HUFA, highly unsaturated fatty acids acids (carbon chain length ≥ C 20 with ≥ 3 double bonds); PL, polar lipid; PUFA, polyunsaturated fatty acids; VO, vegetable oil; TLC, thin-layer chromatography. 2 AbstractThe desaturation and elongation of [1-14 C]18:3n-3 was investigated in hepatocytes from different populations and three different species of salmonids indigenous to Scotland, brown trout, Atlantic salmon and Arctic charr. Two groups of fish were sampled, before and after they were fed two experimental diets, a control diet containing fish oil and a diet containing vegetable oil (a 1:1 blend of linseed oil and rapeseed oil) for 12 weeks. At each sampling time, fatty acyl desaturation and elongation activity was determined in isolated hepatocytes and samples of liver were also collected for lipid compositional analysis. At the initiation of the dietary trial, the liver polar lipid fatty acid compositions of salmon and brown trout were very similar to each other, and the two charr populations were similar to each other having lower total n-3 polyunsaturated fatty acids (PUFA) and 22:6n-3, but higher 20:5n-3 than the other salmonids. Initially, hepatocyte desaturation activity varied with the highest activity in brown trout followed by salmon then charr. Production of 20:5n-3 was particularly high in brown trout. Desaturation of [1-14 C]18:3n-3 was significantly greater in all fish fed the diet containing vegetable oil compared to fish fed the diet containing fish oil. The increase in activity was less in brown trout compared to the other groups of fish. Feeding the vegetable oil diet increased the levels of 18:2n-6, 20:3n-6, total n-6PUFA, 18:3n-3, 18:4n-3, 20:3n-3 and 20:4n-3, and decreased 22:6n-3 and the n-3/n-6 ratio in salmon and brown trout. By contrast, in charr fed the vegetable oil diet, there was no increase in 18:3n-3, 18:4n-3, 20:3n-3 or 20:4n-3 in liver polar lipid and the level of 22:6n-3 was not decreased. In addition, there was only a modest increase in the levels of 18:2n-6 and total n-6PUFA and so the n-3/n-6 ratio was only slightly decreased. The percentage of 20:4n-6, which was not increased in salmon and brown trout fed vegetable oil, was increased in charr fed the vegetable oil diet. Overall, the results indicated that there were significant differences in liver PUFA metabolism between Arctic charr and the other salmonids that could have important consequences, both physiologically and in their ability to be successfully cultured on diets containing vegetable oils.Running title: PUFA composition and desaturation in salmonids.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Hepatocyte fatty acid desaturation and polyunsaturated fatty acid composition of liver in salmonids: effects of dietary vegetable oil

Tocher

Bell

MacGlaughlin

et al. 2001

Comparative Biochemistry and Physiology Part B: Biochemistry an

138

106

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Lipids and Fatty Acids

Tocher

Glencross

2015

Dietary Nutrients, Additives, and Fish Health

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Tropical Vegetable Oils Do Not Alter Growth Performance in African Catfish through a High n‐6 Polyunsaturated Fatty Acids Biosynthesis Capacity

Sourabié

Mandiki

Geay

et al. 2019

Lipids

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The main objective of this study was to determine the best vegetable oils (VO) for nutrition of African catfish by assessing the effects of a complete replacement of fish oil (FO) by different VO sources on its growth performance, fatty acid composition, and elongase-desaturase gene expression levels. Fish (16.2 g of initial body weight) were fed with five experimental isonitrogenous, isolipidic, and isoenergetic diets in which FO was totally replaced by cottonseed oil (CO), palm oil (PO), desert date oil (DO), or Shea butter (SB). Complete replacement of FO with VO did not affect growth performance except for low values in fish fed SB diet. Muscle n-3 LC-polyunsaturated fatty acids (PUFA) were significantly reduced in fish fed VO-based diets when compared with FO fed fish. However, the muscle arachidinic acid (ARA) levels in phospholipid class were 1.4 to 1.6 times higher in fish fed CO and DO diets than FO fed fish despite the lower ARA suppliers from these VO-based diets, suggesting endogenous LC-PUFA biosynthesis from PUFA precursors in fish fed these VO. The fads2 and elovl5 gene expression levels in liver of fish fed DO were also higher compared to FO controls. Therefore, all the results support the hypothesis that African catfish has higher biosynthesis capacity to convert vegetable n-6 PUFA precursors like linoleic acid (LNA, 18:2n-6) into n-6 LC-PUFA of the ARA type, compared to the conversion of vegetable α-linolenic acid (ALA, 18:3n-3) into n-3 LC-PUFA of the eicosapentanoic acid (EPA) or docosahexanoic acid (DHA) type. The results also indicate that DO can be recommended as the best alternative to FO replacement in African catfish nutrition.

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The desaturation and elongation of linolenic acid and eicosapentaenoic acid by hepatocytes and liver microsomes from rainbow trout (Oncorhynchus mykiss) fed diets containing fish oil or olive oil

Cited by 164 publications

References 28 publications

Hepatocyte fatty acid desaturation and polyunsaturated fatty acid composition of liver in salmonids: effects of dietary vegetable oil

Hepatocyte fatty acid desaturation and polyunsaturated fatty acid composition of liver in salmonids: effects of dietary vegetable oil

Lipids and Fatty Acids

Tropical Vegetable Oils Do Not Alter Growth Performance in African Catfish through a High n‐6 Polyunsaturated Fatty Acids Biosynthesis Capacity

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