The design of electrode-array for monitoring the cellular bioimpedance

Lu, Yi-Yu; Huang, Jen-Peng; Cheng, Kuo‐Sheng

doi:10.1109/isiea.2009.5356375

Cited by 7 publications

(6 citation statements)

References 5 publications

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“…Each gold-plated electrode had a width of 1.3 mm with a 6 mil gap between adjacent electrodes. Details of the design and performance of the electrode array were reported in 2009 [25]. In most studies on ECIS, the electrodes are designed to attach at the bottom of the culture plate [7,13].…”

Section: Multi-electrode Culture Monitoring Systemmentioning

confidence: 99%

“…Four-electrode measurement has been performed for the characterization of electrode-array cultures. Previous studies have reported that the adjacent method using fourelectrode measurement is applicable to distinguishing the influence of position error [25].…”

Section: Multi-electrode Culture Monitoring Systemmentioning

confidence: 99%

“…Though electrodes are attached closely to cells in this model, the system has an inherent disadvantage of nonuniform current density distribution. To compensate, a vertical electrode array, that provides more comprehensive interaction within the electric field, was developed [25]. According to simulation results, uniform current density and forward current can be clearly identified using an opposite current method, making it is possible to obtain an objective description of cell activity with a high degree of accuracy.…”

Section: Introductionmentioning

confidence: 99%

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Cell growth characterization using multi-electrode bioimpedance spectroscopy

Huang

et al. 2013

Meas. Sci. Technol.

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Cell growth characterization during culturing is an important issue in a variety of biomedical applications. In this study an electrical bioimpedance spectroscopy-based multi-electrode culture monitoring system was developed to characterize cell growth. A PC12 cell line was cultured for the cell growth study. The bioimpedance variations for PC12 cell growth within the initial 12 h were measured over a range between 1 kHz and 4 MHz at three different medium concentrations. Within this frequency range, the largest bioimpedance value was 1.9 times the smallest bioimpedance value. The phase angle decreased over the range from 1 to 10 kHz when cells were growing. Then, the phase angle approached a constant over the frequency range between 10 kHz and 2 MHz. Thereafter, the phase angle increased rapidly from 20 to 52 degrees during cell culturing between 8 and 12 h at 4 MHz. The maximum cell number after culturing for 12 h increased by 25.8% for the control sites with poly-D-lysine (PDL) pastes. For the normal growth factor, the cell number increased up to 4.78 times from 8 to 12 h, but only 0.96 and 1.60 times for the other two medium growth factors. The correlation coefficients between impedance and cell number were 0.868 (coating with PDL), and 0.836 (without PDL) for the normal concentration medium. Thus, impedance may be used as an index for cell growth characterization.

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Section: Multi-electrode Culture Monitoring Systemmentioning

confidence: 99%

Section: Multi-electrode Culture Monitoring Systemmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cell growth characterization using multi-electrode bioimpedance spectroscopy

Huang

et al. 2013

Meas. Sci. Technol.

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“…Then, 0.5 ml of culturing medium with a density of 8.3 x 10 4 cells/ml PC12 cells are removed and poured into the culture dish. The novel culture dish with 32-electrode-array for real-time measuring the electrical property of cells was proposed and designed [7]. Load PC12 cell with RPMI 1640 medium in designed cultures and cultivate it in well conditioned incubation system for cell growth characterization.…”

Section: A Cell Preparation and Curlturingmentioning

confidence: 99%

3-Dimensional representation for PC12 cell growth characterization

Huang

Cheng

2012

2012 5th International Conference on BioMedical Engineering and Informatics

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In this study, a 3-dimensional representation is employed to characterize electrical property of the cell growth status for PC12. This can extend Electrical Bioimpedance Spectroscopy (EBIS) to multi-electrode application associated with position for increasing the special frequency. Traditionally, 2-dimensional Cole-Cole plot lack for the message of special frequency, especially in multi-electrode applications. A homemade multi-frequency bioimpedance system (MFBii) is integrated for measurement. Results revealed that the impedance between these electrode's pathways can be distinguished a difference from the 3D plot. The electrical property of biological object can be obtained by a set of Cole-Cole plots. This method could offer more information in cell growth, such as cell viability and proliferation monitoring.

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“…In the last few years, many research efforts have been devoted to find a reliable and robust non-invasive technique to estimate and study cell growth on cell-culture assays [ 1 , 2 , 3 , 4 , 5 ] from different viewpoints. Many biomedical setups such as toxicology assays [ 6 ], cancer characterization experiments [ 7 , 8 ], biochemical [ 9 ], immune-assays [ 10 ], stem cells differentiation protocols [ 11 ], etc., seek to quantify the number of cells for characterizing a diversity of research objectives and techniques.…”

Section: Introductionmentioning

confidence: 99%

An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests

Serrano

Huertas

Maldonado-Jacobi

et al. 2018

Sensors

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This paper proposes a new yet efficient method allowing a significant improvement in the on-line analysis of biological cell growing and evolution. The procedure is based on an empirical-mathematical approach for calibration and fitting of any cell-electrode electrical model. It is valid and can be extrapolated for any type of cellular line used in electrical cell-substrate impedance spectroscopy (ECIS) tests. Parameters of the bioimpedance model, acquired from ECIS experiments, vary for each cell line, which makes obtaining results difficult and—to some extent-renders them inaccurate. We propose a fitting method based on the cell line initial characterization, and carry out subsequent experiments with the same line to approach the percentage of well filling and the cell density (or cell number in the well). To perform our calibration technique, the so-called oscillation-based test (OBT) approach is employed for each cell density. Calibration results are validated by performing other experiments with different concentrations on the same cell line with the same measurement technique. Accordingly, a bioimpedance electrical model of each cell line is determined, which is valid for any further experiment and leading to a more precise electrical model of the electrode-cell system. Furthermore, the model parameters calculated can be also used by any other measurement techniques. Promising experimental outcomes for three different cell-lines have been achieved, supporting the usefulness of this technique.

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The design of electrode-array for monitoring the cellular bioimpedance

Cited by 7 publications

References 5 publications

Cell growth characterization using multi-electrode bioimpedance spectroscopy

Cell growth characterization using multi-electrode bioimpedance spectroscopy

3-Dimensional representation for PC12 cell growth characterization

An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests

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