The Design of Sensitive, Selective and Reproducible Biosensor With Enzyme Immobilisation For Analytical Applications

Abstract

The design of sensitive, selective and reproducible biosensors with enzyme immobilization for analytical application is reported. The study is aimed to present the design of sensitive, selective and reproducible biosensors with enzyme immobilization to be used as a versatile and accurate analytical tool for the determination of target analytes in real samples. The biosensor device is developed with the employ of the immobilized enzyme as a catalytic agent to convert the target analyte to a product that can be monitored electrochemically. The biosensors, both in amperometric and potentiometric detection system have been reviewed for different types of analytes. A uric acid biosensor is used as a design example. Analytical performances of the uric acid biosensor have been evaluated in terms of the detection sensitivity, linearity detection range, reproducibility, selectivity, and stability. The potential applications of the biosensor design for analytical purposes are also demonstrated.