Aims: One possible route of transmission of Mycobacterium paratuberculosis from cattle to humans is via contaminated water supplies. The aim of this work was to determine whether this organism can survive standard water treatment processes. Methods and Results: Two strains of M. paratuberculosis (bovine strain, NCTC 8578 and human strain Linda, ATCC 43015) were subjected to various chlorine concentrations (0á5, 1á0 and 2á0 lg ml ±1 ) for 15 and 30 min. Chlorine test solutions were made up in two types of water, sterile water that had been deionized and subjected to reverse osmosis (DRO) and DRO water containing MgCl 2 , CaCl 2 , NaHCO 3 and bovine serum albumin (0á3% w/v), the latter to mimic conditions the organism would experience in commercial water treatment operations. Conclusions: The data showed that when initial inoculum levels were high (10 6 cfu ml ±1 ) neither M. paratuberculosis strain was completely killed at the free chlorine concentrations and contact times applied. Log 10 reductions in the range 1á32±2á82 were observed. The greatest log 10 reduction in cell numbers (2á82 and 2á35 for the bovine and human strains, respectively) was observed at the highest chlorine concentration (2 lg ml ±1 ) and longest contact time (30 min). Signi®cance and Impact of the Study: This work highlights the need for further research into the survival of M. paratuberculosis during water treatment.