The detection and phylogenetic analysis of Anaplasma phagocytophilum-like 1, A. ovis and A. capra in sheep: A. capra divides into two genogroups

Altay, Kürşat; Erol, Ufuk; Şahin, Ömer Faruk; Aytmirzakizi, Ayperi; Temizel, Ethem Mutlu; Aydın, Mehmet Fatih; Dumanlı, Nazir; Aktaş, Münir

doi:10.1007/s11259-022-09998-1

Cited by 10 publications

(10 citation statements)

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“…In recent years, these methods have been widely used in epidemiological studies (46)(47)(48)(49)(50). Although the presence of different TBPs in cattle, sheep, and dogs (HM species, B. vogeli, B. vulpes, A. centrale, A. capra, A. phagocytophilum-like 1, A. ovis, B. major, T. annulata, and T. orientalis) has been previously reported in Kyrgyzstan (14,15,(31)(32)(33)(34)(35)(36), this study is the first molecular study investigating TBPs in horses in the country.…”

Section: Discussionmentioning

confidence: 99%

“…To date, no record of equine-TBPs has been found in the literature in Kyrgyzstan. Recently, it has been reported first molecular presence and prevalence of the important TBPs such as canine and bovine hemotropic mycoplasma species ( 31 , 32 ), B. vogeli, B. vulpes in dogs ( 33 ), A. centrale, A. capra , A. phagocytophilum -like 1, B. major, T. annulata, and T. orientalis in cattle ( 14 , 15 , 34 , 35 ), A. ovis, A. capra , and A. phagocytophilum -like 1 in sheep ( 36 ) in Kyrgyzstan. In this study, we aimed to survey of TBPs (EP, HM, Anaplasma phagocytophilum, and Anaplasma capra ) in grazing horses in Kyrgyzstan and to detect the genetic diversity of the pathogens.…”

Section: Introductionmentioning

confidence: 99%

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Survey of tick-borne pathogens in grazing horses in Kyrgyzstan: phylogenetic analysis, genetic diversity, and prevalence of Theileria equi

Altay,

Erol,

Sahin

et al. 2024

Front. Vet. Sci.

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IntroductionTick-borne pathogens (TBP) are an important group of organisms that can affect animals and humans all over the world. Equine piroplasmosis (EP), caused by Theileria equi and Babesia caballi, is considered one of the most important tick-borne diseases and can cause significant clinical symptoms and mortality in horses. Moreover, EP plays a restrictive role in international horse traditions and transportation. Although these species can cause similar symptoms, there are different 18S rRNA genotypes of T. equi (five genotypes) and B. caballi (three genotypes). Besides piroplasma species, Anaplasma and hemotropic mycoplasmas (HM) are known as other important tick-borne pathogens reported in horses.MethodsIn this study, we investigated the presence, prevalence, genetic diversity, and phylogenetic analyses of TBPs using PCRs and DNA sequencing in grazing horses in Kyrgyzstan. For these purposes, a total of 311 blood samples were collected from Chuy, Issyk-Kul, Naryn, Osh, Talas, and Jalal-Abad.ResultsDNA amplification of TBP revealed that 23 (7.40%) out of 311 samples were found to be positive for T. equi. However, B. caballi, HM, A. phagocytophilum, and A. capra were not detected in this study. The infection rate of T. equi was higher in males (8.11%) than in females (6.35%) (p=0.2880) and in those older than 5 years (9.02%) than in the 1-4 age group (6.35%) (p=0.1950). Phylogenetic analysis of 18S rRNA revealed that A and E genotypes of T. equi have circulated in grazing horses in Kyrgyzstan.DiscussionInformation about the genetic diversity of T. equi is important for understanding the population dynamics of the species and developing effective control strategies against this pathogen. This is the first molecular investigation of A. capra in horses in Kyrgyzstan. Although this pathogen has been detected in different hosts in Kyrgyzstan, it was not detected in this study. However, considering the wide host spectrum of A. capra, it is thought that more large-scale studies are needed to understand the effect of horses on the epidemiology of this pathogen.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Survey of tick-borne pathogens in grazing horses in Kyrgyzstan: phylogenetic analysis, genetic diversity, and prevalence of Theileria equi

Altay,

Erol,

Sahin

et al. 2024

Front. Vet. Sci.

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“…Both the clinical signs are not fully descriptive in the diagnostic attempts of veterinarians, and the difficulties in identifying the species found in this lineage have been overcome by molecular methods. PCR and sequencing are widely used in diagnosis and in determining the phylogenetic positions of species [ 22 , 23 , 30 ]. Studies using these methods have provided more accurate information about the epidemiology of the disease as well as the identification of species.…”

Section: Discussionmentioning

confidence: 99%

“…The improvement of molecular identification techniques and the widespread use of these for the detection of parasitic diseases, especially in the last two decades, have provided important contributions to research into the high specificity and sensitivity diagnosis of parasitic diseases, the determination of the genetic diversity of pathogens, and the detection of new species/strains/genotypes [ 15 , 22 , 23 , 30 ]. Furthermore, molecular techniques can provide unexpected species detection in different host species [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, we reported the first molecular presence and prevalence of Dirofilaria immitis ( D. immitis ) and D. repens [ 16 ], canine and bovine hemotropic mycoplasma species [ 17 , 18 ], and canine hepatozoonozis in dogs from Kyrgyzstan [ 19 ]. On the other hand, the presence of T. orientalis, T. annulata, B. major [ 20 , 21 ], Anaplasma centrale ( A. centrale ) , A. phagocytophilum -like 1, A. capra , and A. ovis were revealed in cattle and sheep [ 22 , 23 ]. In this study, we aimed to detect Babesia species infecting shelter dogs in Kyrgyzstan and to confirm the species of the parasite detected through sequencing.…”

Section: Introductionmentioning

confidence: 99%

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First Molecular Evidence of Babesia vogeli, Babesia vulpes, and Theileria ovis in Dogs from Kyrgyzstan

et al. 2023

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Tick-borne parasitic diseases cause mild to severe infections among vertebrate hosts, including dogs. Species in the genus Babesia are important tick-borne pathogens and have worldwide distributions. Although there are data on the prevalence and distribution of Babesia species among dogs around the world, there is no information available in Kyrgyzstan, according to a literature review. In this study, 337 dogs were screened by nested PCR for the presence of the 18S small subunit ribosomal RNA (18S SSU rRNA) gene of piroplasm species. Overall prevalence was 6.23% (21/337) for Babesia/Theileria spp. DNA sequencing of positively tested samples revealed that eighteen samples were infected with Babesia vogeli (B. vogeli) (5.34%), two samples with B. vulpes (0.59%), and one sample with Theileria ovis (T. ovis) (0.29%). The phylogenetic analyses and nucleotide sequences in contrast with those present in GenBank revealed that two nucleotide substitutions (594th and 627th) were found between B. vogeli isolates, including ours, indicating that the mutation is relatively rare. The sequences of other pathogens obtained in this study confirmed 100% nucleotide identity with B. vulpes and T. ovis sequences in GenBank. To the best of our knowledge, B. vogeli, B. vulpes, and T. ovis were detected for the first time in dogs from Kyrgyzstan, and it is thought that results will contribute to the understanding of the epidemiology of canine tick-borne pathogens in the country.

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A comprehensive molecular survey of vector-borne blood parasites in cattle in Kyrgyzstan with a note of the first molecular detection of Anaplasma bovis and Candidatus Anaplasma Camelii

Altay,

Abdugani,

Sahin

et al. 2024

Trop Anim Health Prod

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The detection and phylogenetic analysis of Anaplasma phagocytophilum-like 1, A. ovis and A. capra in sheep: A. capra divides into two genogroups

Cited by 10 publications

References 49 publications

Survey of tick-borne pathogens in grazing horses in Kyrgyzstan: phylogenetic analysis, genetic diversity, and prevalence of Theileria equi

Survey of tick-borne pathogens in grazing horses in Kyrgyzstan: phylogenetic analysis, genetic diversity, and prevalence of Theileria equi

First Molecular Evidence of Babesia vogeli, Babesia vulpes, and Theileria ovis in Dogs from Kyrgyzstan

A comprehensive molecular survey of vector-borne blood parasites in cattle in Kyrgyzstan with a note of the first molecular detection of Anaplasma bovis and Candidatus Anaplasma Camelii

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