“…Sufficient lipid together with -0.05 pCi of lZ5I-marker per experimental animal was dried and suspended in buffer by mechanical agitation, yielding large multilamellar liposomes. In most cases these liposomes were subsequently extruded through polycarbonate membranes4 having 8-, 5-, 3-, 2-, and 1-pm diameter pore sizes to generate populations having different mean diameters (17).…”