The development and validation of liquid chromatography method for the simultaneous determination of metformin and glipizide, gliclazide, glibenclamide or glimperide in plasma

AbuRuz, Salah; Millership, Jeffrey S.; McElnay, James

doi:10.1016/j.jchromb.2004.12.018

Cited by 158 publications

(92 citation statements)

References 9 publications

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“…MET To the best of our knowledge no method is reported in literature for simultaneous determination of MET and REPA by high performance liquid chromatography (HPLC). Several HPLC methods have been reported in the literature for quantitative determination of MET alone [4] or in combination with other drugs in tablets [5][6][7], human serum and other biological fluids [8][9]. Similarly, a few HPLC methods have been reported for the quantitative determination of REPA in tablets alone or in combination with other drugs [10][11][12], and in human serum and other biological fluids [13,14].…”

Section: Introductionmentioning

confidence: 99%

Validated stability-indicating RP-HPLC UV method for simultaneous determination of metformin and repaglinide

Joshi

Nahire

Shastri

et al. 2012

Acta Chromatographica

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Summary.A simple, rapid, precise, and accurate, stability-indicating reversed phase high performance liquid chromatographic method was developed and validated for simultaneous determination of metformin HCl and repaglinide. The chromatographic separation was achieved on YMC Pack AM ODS (5 μm, 250 mm length × 4.6 mm i.d.) column at a detector wavelength of 210 nm, using an isocratic mobile phase consisting of methanol and 10 mM potassium dihydrogen phosphate buffer (pH 2.5) in a ratio of 70:30 v/v at a flow rate of 1 mL min −1 . The retention times for metformin and repaglinide were found to be 2.6 and 11.3 min, respectively. The drugs were exposed to thermal, photolytic, hydrolytic, and oxidative stress conditions, and the stressed samples were analyzed by the proposed method. Validation of the method was carried out as per International Conference on Harmonization (ICH) guidelines. Linearity was established for metformin and repaglinide in the range of 5-200 μg mL −1 and 1-200 μg mL −1 , respectively. The limits of detection were 0.3 μg mL −1 and 0.13 μg mL −1 for metformin and repaglinide, respectively. The method was found to be specific and stability-indicating as no interfering peaks of degradants and excipients were observed. The proposed method is hence suitable for application in quality-control laboratories for quantitative analysis of both the drugs individually and in combination, since it is simple and rapid with good accuracy and precision.

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Section: Introductionmentioning

confidence: 99%

Validated stability-indicating RP-HPLC UV method for simultaneous determination of metformin and repaglinide

Joshi

Nahire

Shastri

et al. 2012

Acta Chromatographica

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“…Literature survey revealed HPLC, RP-HPLC, LC-MS, Spectrofluorimetric and simultaneous UV spectrophotometric methods are reported for the estimation of metformin hydrochloride [2][3][4][5], pioglitazone hydrochloride [6][7][8][9] and glibenclamide [10] alone or in combination with other anti-diabetic agents. So the present study aim to develop a simple, selective and precise RP-HPLC method for the simultaneous estimation of MET, PIO and GLI in bulk drug samples and in combined dosage formulation.…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Metformin Hydrochloride, Pioglitazone Hydrochloride and Glibenclamide in Bulk and Pharmaceutical Dosage Forms

S¹

2014

IOSRJPBS

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“…It stimulates the secretion and enhances the utilization of insulin by appropriate tissues. Different methods have been reported for the analysis of Glibenclamide alone or its combination with other drugs is HPLC [19][20][21][22][23][24], voltametric [25], spectophotometry [25] and LCMS/ MS [26].…”

Section: Introductionmentioning

confidence: 99%

Method Development and Validation of Metformine, Pioglitazone and Glibenclamide in Tablet Dosage Form by using RP-HPLC

Tengli¹

2013

Biochem Anal Biochem

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Reverse phase high performance liquid chromatographic method has developed for the simultaneous estimation of metformine, pioglitazone and glibenclamide in tablet dosage for using gliclazide as an internal standard. The separation was achieved at ambient temperature with low pressure gradient mode by using 5 µ size phenomenex luna CN (100R 250×4.60 (mm) column with mobile phase containing acetonitrile, water and buffer (0.5% potassium dihydrogen phosphate) pH 2.5 adjusted with orthophosphoic acid in the ratio of 60:20:20. The flow rate was 1 mL min -1 and eluent was monitored at 230 nm by using UV detector. The selected chromatographic conditions effectively separated Metformine, pioglitazone and Glibenclamide with the retention time of 2.2, 2.8 and 5.8 min respectively. The linearity range for metformine, pioglitazone and glibenclamide is found in the range of 50-300 µg mL -1 , 1.5-9.0 µg mL -1 and 0.5-3.0 µg mL -1 respectively. The developed method was found to be accurate, simple, specific and reproducible. It can also be used for routine quality control analysis of these antidiabetic drugs in combinational dosage forms.

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The development and validation of liquid chromatography method for the simultaneous determination of metformin and glipizide, gliclazide, glibenclamide or glimperide in plasma

Cited by 158 publications

References 9 publications

Validated stability-indicating RP-HPLC UV method for simultaneous determination of metformin and repaglinide

Validated stability-indicating RP-HPLC UV method for simultaneous determination of metformin and repaglinide

Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Metformin Hydrochloride, Pioglitazone Hydrochloride and Glibenclamide in Bulk and Pharmaceutical Dosage Forms

Method Development and Validation of Metformine, Pioglitazone and Glibenclamide in Tablet Dosage Form by using RP-HPLC

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