The development and validation of a multiclass liquid chromatography tandem mass spectrometry (LC–MS/MS) procedure for the determination of veterinary drug residues in animal tissue using a QuEChERS (QUick, Easy, CHeap, Effective, Rugged and Safe) approach

Stubbings, G. W.; Bigwood, Timothy

doi:10.1016/j.aca.2009.01.029

Cited by 320 publications

(157 citation statements)

References 16 publications

Supporting

Mentioning

151

Contrasting

Unclassified

Order By: Relevance

“…[20][21][22][23][24] The MMM of Kaufmann et al [25] was also performed, but it was deemed to be too laborious and time-consuming for our purposes. Each method had advantages in some respect over the others.…”

Section: Comparison Of Different Methodsmentioning

confidence: 99%

“…MMM 4 was from Stubbings et al [23] with slight modifications. The 2 g samples in 50-ml PP tubes were homogenized with 6 ml of MeCN containing 1% acetic acid.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Table 3 shows that our concerns were misplaced, at least for the majority of Table 2a. Summary of qualitative results in the comparison of 5 MMMs for the 62 veterinary drugs in bovine kidney: drugs that gave >2 nonidentifications (false negatives) among 22 sample extracts Mol et al [20] Martos et al [21] Mastovska & Lightfield [22] Stubbings et al [23] Chiaochan et al [24] Erythromycin [20] 23 Amoxicillin; Ampicillin; Carbadox; Cefazolin; Chlortetracycline; Cloxacillin; Dicloxacillin; Erythromycin; Florfenicol Amine; Oxacillin; Ractopamine; Salbutamol; Sulfanilamide; Sulfapyridine; Tetracyline; Tylosin Martos et al [21] 20 Ampicillin; Carbadox; Chlortetracycline; Cloxacillin; Difloxacin; Enrofloxacin; Erythromycin; Florfenicol Amine; Oxacillin; Oxyphenylbutazone; Oxytetracycline; Sulfanilamide; Tetracyline Mastovska & Lightfield [22] 25 Amoxicillin; Ampicillin; Cefazolin; Chlortetracycline; Ciprofloxacin; Clindamycin; Cloxacillin; Dicloxacillin; Gamithromycin; Oxacillin; Oxyphenylbutazone; Oxytetracycline; Pirlimycin; Sulfanilamide; Tetracyline; Tilmicosin; Tulathromycin; Tylosin Stubbings et al [23] 21 Amoxicillin; Ampicillin; Beta/Dexamethasone; Carbadox; Cefazolin; Chloramphenicol; Chlortetracycline; Erythromycin; Oxyphenylbutazone; Oxytetracycline; Pirlimycin; Tetracyline; Tylosin Chiaochan et al [24] 26 Amoxicillin; Cefazolin; Clindamycin; Cloxacillin; Dicloxacillin; Erythromycin; Florfenicol Amine; Flunixin; Gamithromycin; Oxacillin; Oxyphenylbutazone; Salbutamol; Sulfabromomethazine; Sulfanilamide; Tilmicosin; Tulathromycin; Tylosin; Zeranol; Zilpaterol drug analytes determined in the 10 real samples provided. As shown in the table, the use of a 5 min shake gave the highest concentrations 17 out of the 26 instances when a drug was determined in the samples.…”

Section: Analysis Of Incurred Samplesmentioning

confidence: 99%

See 2 more Smart Citations

Development and validation of a streamlined method designed to detect residues of 62 veterinary drugs in bovine kidney using ultra‐high performance liquid chromatography – tandem mass spectrometry

Lehotay

Lightfield

Geis-Asteggiante³

et al. 2012

Drug Testing and Analysis

View full text Add to dashboard Cite

In the USA, the US Department of Agriculture's Food Safety and Inspection Service (FSIS) conducts the National Residue Program designed to monitor veterinary drug and other chemical residues in beef and other slaughtered food animals. Currently, FSIS uses a 7-plate bioassay in the laboratory to screen for antimicrobial drugs in bovine kidneys from those animals tested positive by inspectors in the slaughter establishments. The microbial inhibition bioassay has several limitations in terms of monitoring scope, sensitivity, selectivity, and analysis time. Ultra-high performance liquid chromatography -tandem mass spectrometry (UHPLC-MS/MS) has many advantages over the bioassay for this application, and this study was designed to develop, evaluate, and validate a fast UHPLC-MS/MS method for antibiotics and other high-priority veterinary drugs in bovine kidney. Five existing multi-class, multi-residue methods from the literature were tested and compared, and each performed similarly. Experiments with incurred samples demonstrated that a 5-min shake of 2 g homogenized kidney with 10 ml of 4/1 (v/v) acetonitrile/water followed by simultaneous clean-up of the initial extract with 0.5 g C18 and 10 ml hexane gave a fast, simple, and effective sample preparation method for the <10 min UHPLC-MS/MS analysis. An extensive 5-day validation process demonstrated that the final method could be used to acceptably screen for 54 of the 62 drugs tested, and 50 of those met qualitative MS identification criteria. Quantification was not needed in the application, but the method gave ≥70% recoveries and ≤25% reproducibilities for 30 of the drugs. Published 2012. This article is a U.S. Government work and is in the public domain of the USA.

show abstract

Section: Comparison Of Different Methodsmentioning

confidence: 99%

“…MMM 4 was from Stubbings et al [23] with slight modifications. The 2 g samples in 50-ml PP tubes were homogenized with 6 ml of MeCN containing 1% acetic acid.…”

Section: Sample Preparationmentioning

confidence: 99%

Section: Analysis Of Incurred Samplesmentioning

confidence: 99%

See 1 more Smart Citation

Development and validation of a streamlined method designed to detect residues of 62 veterinary drugs in bovine kidney using ultra‐high performance liquid chromatography – tandem mass spectrometry

Lehotay

Lightfield

Geis-Asteggiante³

et al. 2012

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…The use of anhydrous sodium sulphate in sample clean up when extracting these analytes has been previously seen. [Stubbings et al, 2009]. Hence the use of anhydrous sodium sulphate was investigated followed by addition of a hexane wash step.…”

Section: Sample Extraction Developmentmentioning

confidence: 99%

Development and validation of a rapid multi-class method for the confirmation of fourteen prohibited medicinal additives in pig and poultry compound feed by liquid chromatography–tandem mass spectrometry

Cronly

Behan²,

Foley³

et al. 2010

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

A confirmatory method has been developed to allow for the analysis of fourteen prohibited medicinal additives in pig and poultry compound feed. These compounds are prohibited for use as feed additives although some are still authorised for use in medicated feed. Feed samples are extracted by acetonitrile with addition of sodium sulphate. The extracts undergo a hexane wash to aid with sample purification. The extracts are then evaporated to dryness and reconstituted in initial mobile phase. The samples undergo an ultracentrifugation step prior to injection onto the LC-MS/MS system and are analysed in a run time of 26 minutes. The LC-MS/MS system is run in MRM mode with both positive and negative electrospray ionisation. The method was validated over three days and is capable of quantitatively analysing for metronidazole, dimetridazole, ronidazole, ipronidazole, chloramphenicol, sulfadiazine, sulfamethazine, dinitolimide, ethopabate, carbadox and clopidol. The method is also capable of qualitatively analysing for tylosin, virginiamycin and avilamycin. A level of 100 µg kg -1 was used for validation purposes and the method is capable of analysing to this level for all the compounds. Validation criteria of trueness, precision, repeatability and reproducibility along with measurement uncertainty are calculated for all analytes.

show abstract

“…Acidification could also help disrupt the compoundprotein binding in biological samples; thus, it has been widely used in the extraction of various compounds [49]. In this study, different percentages (0, 1, and 5 %) of AA in MeCN were tested in order to acquire satisfactory recoveries.…”

Section: Influence Of Extraction Aa Contentmentioning

confidence: 99%

Simultaneous determination of 24 personal care products in fish muscle and liver tissues using QuEChERS extraction coupled with ultra pressure liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometer analyses

et al. 2016

View full text Add to dashboard Cite

A sensitive and selective quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction combined with dispersive solid-phase extraction (d-SPE) cleanup method was developed to simultaneously extract a wide range of personal care products (16 biocides, 4 synthetic musks, and 4 benzotriazoles) in fish muscle and liver tissues. In order to get satisfactory recoveries, different extraction parameters were optimized, including extraction salts and d-SPE materials, extraction solvents and acetic acid contents in organic phase, and the ratios of solvent and water. Ultra pressure liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry were used to analyze the target compounds in the extracts. Among the 24 personal care products, the recoveries in the range of 70-120 % were obtained for 20, 19, and 12 analytes in fish muscle at the spiking concentrations of 10, 5, and 1 ng/g ww, respectively, and for 13, 12, and 11 analytes in liver at the spiking concentrations of 40, 20, and 4 ng/g ww, respectively. Method quantification limits (MQLs) of all analytes were 0.02-2.12 ng/g ww for fish muscle and 0.22-12.2 ng/g ww for fish liver tissues. The method was successfully applied to wild fish samples collected from Dongjiang River, south China. Twenty-one and 17 of the analytes were found in fish muscle and liver samples, respectively, in at least one site of the river with the concentrations between below MQLs and 119 ng/g ww, respectively.

show abstract

The development and validation of a multiclass liquid chromatography tandem mass spectrometry (LC–MS/MS) procedure for the determination of veterinary drug residues in animal tissue using a QuEChERS (QUick, Easy, CHeap, Effective, Rugged and Safe) approach

Cited by 320 publications

References 16 publications

Development and validation of a streamlined method designed to detect residues of 62 veterinary drugs in bovine kidney using ultra‐high performance liquid chromatography – tandem mass spectrometry

Development and validation of a streamlined method designed to detect residues of 62 veterinary drugs in bovine kidney using ultra‐high performance liquid chromatography – tandem mass spectrometry

Development and validation of a rapid multi-class method for the confirmation of fourteen prohibited medicinal additives in pig and poultry compound feed by liquid chromatography–tandem mass spectrometry

Simultaneous determination of 24 personal care products in fish muscle and liver tissues using QuEChERS extraction coupled with ultra pressure liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometer analyses

Contact Info

Product

Resources

About