The development of a bin mapping population and the selective mapping of 103 markers in the diploidFragariareference map

Abstract

We have identified a set of plants (the bin set) to permit "selective" or "bin" mapping using the diploid strawberry mapping population FV x FN, derived from the F2 cross F. vesca 815 x F. nubicola 601, which has been used to develop the Fragaria reference map. The bin set consists of 8 plants: the F. vesca 815 parent, the F1 hybrid individual, and 6 seedlings of the F2 population. This bin set divides the 578 cM of the diploid Fragaria genome into 46 bins, the largest mapping bin being 26 cM in length and the average bin size being 12.6 cM. To validate the FV x FN bin set, we used it to locate 103 loci into bins on the FV x FN map. These loci comprised 61 previously described SSRs, 38 new SSRs developed in this investigation from Fragaria x ananassa genomic DNA, EST and gene sequences, and 4 ripening-related genes developed for Prunus. The 103 markers were located to bins on all 7 linkage groups of the Fragaria map and a new mapping bin was identified with the novel markers, demonstrating that the map covers the majority of the diploid Fragaria genome and that the 6 bin-set seedlings selected were appropriate for bin mapping using this progeny.

Genetic Analysis of Strawberry Fruit Aroma and Identification ofO-Methyltransferase FaOMTas the Locus Controlling Natural Variation in Mesifurane Content

“…QTLs for g-decalactone (82III-2) and g-dodecalactone (87III-1), two volatiles significantly correlated (r = 0.42) and clustered in the HCA, did not colocate in the same LG and were mapped to different homoeologous LGs of HG III (Supplemental Tables S1 and S4;. In agreement with their common enzymatic reaction, QTLs for different esters b Bin map position in the diploid Fragaria reference map according to Sargent et al (2008). Tables S2-S4).…”

Comparative Genetic Mapping Points to Different Sex Chromosomes in Sibling Species of Wild Strawberry (Fragaria)

“…DNA was diluted to 0.03 ng/ml with distilled deionized water for PCR reactions. Because we were interested in testing synteny, rather than creating a genetic map of the whole genome, we used 56 primer pairs for DNA markers (SSRs or genes) that have mapped either to the homeologous group (HG) in F. virginiana to which the protosex chromosome belongs (i.e., HG VI; Spigler et al 2010) or to the homeologous linkage group (LG) in a diploid interspecific cross (i.e., LG 6; Sargent et al 2006Sargent et al , 2008. PCR was performed using the Poor Man's PCR protocol (Schuelke 2000) as previously described (Spigler et al 2008).…”

Breeding rootstocks for Prunus species: Advances in genetic and genomics of peach and cherry as a model

“…Le Dantec et al (2010) used the progeny of the J×F mapping in peach (Dirlewanger et al, 2006) and the diploid set of bin mapping of Fragaria (Sargent et al, 2008) to identify candidate genes involved in the organoleptic quality of peach fruit and perform syntenic analysis between two genera, which can be expressed as the conservation or coherence of markers and genes and their order on the chromosomes of different genomes. They constructed two cDNA libraries from fruits of 'Fantasia' at two stages of development (Dirlewanger et al, 1998;Dirlewanger et al, 2006).…”