The development of a matrix metalloproteinase-1 biosensor based on the surface plasmon resonance imaging technique

Tokarzewicz, Anna; Romanowicz, Lech; Sveklo, I.; Gorodkiewicz, Ewa

doi:10.1039/c6ay01856d

Cited by 32 publications

(34 citation statements)

References 19 publications

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“…As interferents, human albumin, MMP-1, MMP-2, cathepsin B, cathepsin D, cathepsin E, and cathepsin G were used. The choice of these interferents was made because of the fact that albumin is the most common protein in human body fluids [21]; MMP-1, MMP-2, as the CTSL, which are proteases, take part in the degradation of the extracellular proteins [17]; cathepsin B, D, E, G are compounds belonging to the cathepsin group as the cathepsin L.…”

Section: Resultsmentioning

confidence: 99%

“…During the quantitative determination of the different proteins by the SPRI technique two, different types of an immobilization are used: antibody—antigen (covalent type of the immobilization, e.g., [17]) or inhibitor—enzyme (hydrophobic type of the immobilization, e.g., [18]). For the quantification of the CTSL, the enzyme—inhibitor (RKLLW-NH 2 ) interaction was used.…”

Section: Introductionmentioning

confidence: 99%

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A New Analytical Method for Determination of Cathepsin L Based on the Surface Plasmon Resonance Imaging Biosensor

Tokarzewicz

Romanowicz

Sankiewicz

et al. 2019

IJMS

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The purpose of this study was to develop a new method for a determination of the cathepsin L—biosensor based on the Surface Plasmon Resonance Imaging technique. The cathepsin L is an endopeptidase, which degrades proteins and plays an important role in various processes occurring in the human body. The detection technique, Surface Plasmon Resonance Imaging, is an optical, label-free technique, which can be used for quantitative determination of the different proteins. In order to bind the enzyme, the cathepsin L inhibitor—RKLLW-NH2 was used. The validation process showed that parameters: precision, accuracy, and selectivity of the method were acceptable. The analytically useful range of the standard curve was 0.50 ng/mL—15.00 ng/mL. The detection and quantification limit of method was 1.67 pg/mL and 5.07 pg/mL, respectively. The usefulness of the developed method was confirmed by the determination of the cathepsin L concentration in the blood plasma of some healthy persons and in the blood plasma of patients. The obtained results were compared with the results obtained by the ELISA. It was found that the correlation between these two methods was very strong, what suggest that the developed method can be used as the competitive method to the ELISA.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A New Analytical Method for Determination of Cathepsin L Based on the Surface Plasmon Resonance Imaging Biosensor

Tokarzewicz

Romanowicz

Sankiewicz

et al. 2019

IJMS

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“…Current interest in MMPs and fibronectin in saliva suggested the necessity for further investigations. Therefore, in our study we wanted to investigate the levels of MMP-1, MMP-2 and fibronectin in the saliva of patients with caries, planned for endodontic treatment or surgical extraction using the Surface Plasmon Resonance (SPR) imaging method [25][26][27][28].…”

Section: Fibronectinmentioning

confidence: 99%

Levels of Selected Matrix Metalloproteinases—MMP-1, MMP-2 and Fibronectin in the Saliva of Patients Planned for Endodontic Treatment or Surgical Extraction

Matuszczak

Cwalina²,

Tylicka

et al. 2020

JCM

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Objectives: Composition of saliva reflects the condition of the oral cavity. The aim of the study: Investigation of the concentrations of MMP-1 (Matrix metalloproteinase-1), MMP-2 (Matrix metalloproteinase-2) and fibronectin in the saliva of patients planned for endodontic treatment or surgical extraction. Material and methods: Seventy-five patients with caries and 14 healthy subjects were included in the study. Subjects were divided into group 1, in which 50 patients were planned for endodontic treatment, and group 2, in which 25 patients were planned for surgical extraction. For the measurements, we used a surface plasmon resonance imaging biosensor. Results: We found higher levels of MMP-1, MMP-2 and fibronectin in the saliva of patients planned for dental treatment than in healthy donors. We found lower concentrations of MMP-2 in subjects planned for surgical extraction, than in patients planned for endodontic treatment; however, there were no such differences in salivary concentrations of MMP-1 and fibronectin. There were no statistically significant differences in MMP-1 concentrations in the saliva before and after any type of dental treatment, but contrary to that, we found a statistically significant decrease in MMP-2 concentrations after endodontic treatment and after surgical extraction. We found a significant rise in the concentrations of fibronectin after surgical extraction but not after endodontic treatment. Conclusions: The concentrations of MMP-1 and MMP-2 in the saliva of our patients with caries were increased in comparison to healthy individuals, but after the treatment—so sanation of the oral cavity—we noted a decrease in matrix metalloproteinases (MMPs) levels. MMPs can be found in gingival crevicular fluid and saliva, carious dentin and plaque. According to our observations, the main source of MMPs in patients with caries is probably carious dentin. Increase in the salivary levels of fibronectin (FN) after surgical extraction may be connected with soft tissue injury caused by surgical extraction. Our results are another example of the fact that higher salivary concentrations of MMP-1, MMP-2 and FN can reflect the health status of the oral cavity in patients with caries.

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“…The papers pertaining to stages (iii) and (iv) represent complete analytical procedures [14,15,16,17,18,19,20,21,22,23,24,25,26,27]. These biosensors and related analytical procedures are ready to be subjected to clinical investigation for subsequent use in diagnosis.…”

Section: Stages Of Biosensor Developmentmentioning

confidence: 99%

“…Example of covalent antibody immobilization ( c ) and picture of the chip ( a ) and image of the chip obtained by a CCD camera ( b ). Reproduced from [22] with permission of Royal Society of Chemistry.…”

Section: Figurementioning

confidence: 99%

Recent Progress in Surface Plasmon Resonance Biosensors (2016 to Mid-2018)

Gorodkiewicz

Łukaszewski

2018

Biosensors

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More than 50 papers on surface plasmon resonance biosensors, published between 2016 and mid-2018, are reviewed. Papers concerning the determination of large particles such as vesicles, exosomes, cancer cells, living cells, stem cells, and microRNA are excluded, as these are covered by a very recent review. The reviewed papers are categorized into five groups, depending on the degree of maturity of the reported solution; ranging from simple marker detection to clinical application of a previously developed biosensor. Instrumental solutions and details of biosensor construction are analyzed, including the chips, receptors, and linkers used, as well as calibration strategies. Biosensors with a sandwich structure containing different nanoparticles are considered separately, as are SPR (Surface Plasmon Resonance) applications for investigating the interactions of biomolecules. An analysis is also made of the markers determined using the biosensors. In conclusion, there is shown to be a growing number of SPR applications in the solution of real clinical problems.

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The development of a matrix metalloproteinase-1 biosensor based on the surface plasmon resonance imaging technique

Abstract: The purpose of this study was to create a new, highly selective biosensor, which uses surface plasmon resonance as a detection method for the quantitative determination of the matrix metalloproteinase-1.

Cited by 32 publications

References 19 publications

A New Analytical Method for Determination of Cathepsin L Based on the Surface Plasmon Resonance Imaging Biosensor

A New Analytical Method for Determination of Cathepsin L Based on the Surface Plasmon Resonance Imaging Biosensor

Levels of Selected Matrix Metalloproteinases—MMP-1, MMP-2 and Fibronectin in the Saliva of Patients Planned for Endodontic Treatment or Surgical Extraction

Recent Progress in Surface Plasmon Resonance Biosensors (2016 to Mid-2018)

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