The development of a matrix-assisted laser desorption/ionization mass spectrometry-based method for the protein fingerprinting and identification of Aeromonas species using whole cells

Donohue, Maura J.; Smallwood, Anthony W.; Pfaller, Stacy; Rodgers, Mark R.; Shoemaker, Jody A.

doi:10.1016/j.mimet.2005.08.005

Cited by 78 publications

(71 citation statements)

References 33 publications

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“…The relation of the signatures of the Aeromonas strains to each other is graphically depicted (Fig. 2) according to the degrees of association (Donohue et al, 2006;Eddabra et al, 2012). Although designed primarily for clinical specimens, API 20E has found its way in identification of environmental isolates, particularly fish bacterial isolates (Topic Popovic et al, 2007).…”

Section: Methodological Differences In Strain Discriminationmentioning

confidence: 99%

Detection and diversity of aeromonads from treated wastewater and fish inhabiting effluent and downstream waters

Popović

Kazazić

Strunjak‐Perović

et al. 2015

Ecotoxicology and Environmental Safety

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a b s t r a c tA two-season investigation of the wastewater treatment plant (WWTP) effluent, of related waters, sludge and fish across a wide area and 11 stations, with emphasis on Aeromonas spp. was conducted. Aeromonas veronii was the prevailing aeromonad isolated by MALDI TOF MS in the summer period. A rise of Aeromonas hydrophila was observed in summer in raw sewage, treated wastewater and effluent-carrying canal. The ratio of aeromonad species retrieved from fish tissues did not correspond with the water and sludge findings, as in spring in the effluent-carrying canal fish carried Aeromonas salmonicida ssp. salmonicida and Aeromonas bestiarum, while in summer mainly A. veronii and Acinetobacter johnsonii were isolated from fish tissues in the same location. No correlation was established between fecal coliforms/ enterococci and aeromonad occurrence. All retrieved Aeromonas species demonstrated a distinct spectral pattern, with peaks showing unique mass distribution ranging from 4000 to 10,000 Da. Hierarchical clustering separated aeromonads of all isolated species and clustered closely related strains together. Resistance was determined towards amoxicillin, and frequently towards sulfamethoxazole and erythromycin. In summer, a high proportion of water and sludge Aeromonas species demonstrated multiple resistance patterns towards five or more antimicrobials. The quinolone resistance of water aeromonads was mostly related to A. veronii. There are potential health concerns regarding aeromonad exposure amongst recreational fishermen who come into contact with fish inhabiting waters downstream from the WWTP, and WWTP workers who are occupationally exposed to wastewaters and their aerosols.

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Section: Methodological Differences In Strain Discriminationmentioning

confidence: 99%

Detection and diversity of aeromonads from treated wastewater and fish inhabiting effluent and downstream waters

Popović

Kazazić

Strunjak‐Perović

et al. 2015

Ecotoxicology and Environmental Safety

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“…The correct identification of the 17 isolates was dependent on the presence of the reference strains in the MALDI-bioTyper 3.0 database because the species of the reference strain will give the closest match for the identification of the tested strain. The use of MALDI-TOF MS is reported as suitable for the identification of anaerobic bacteria (Veloo et al, 2011 MALDI-TOF MS fingerprinting, a higher discrimination potential has been described, allowing the differentiation and correct identification of much close bacterial species and even strains of the same species (Keys et al, 2004;Donohue et al, 2006;Vargha et al, 2006).…”

Section: Identification Of Isolates Using Maldi-tof Msmentioning

confidence: 99%

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

Makete

Aiyegoro

Thantsha

2016

Probiotics & Antimicro. Prot.

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“…Although accurate identification of Aeromonas species could be achieved using the nucleotide sequences of housekeeping genes (7,10), these molecular methods are labor-intensive and timeconsuming. Recently, several studies have shown that the matrixassisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) can rapidly and accurately identify different Aeromonas species (13)(14)(15). However, among aeromonads that are studied using this method, species identification of A. dhakensis rarely has been discussed.…”

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confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Can Accurately Differentiate Aeromonas dhakensis from A. hydrophila, A. caviae, and A. veronii

Chen

Lee

et al. 2014

J Clin Microbiol

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The development of a matrix-assisted laser desorption/ionization mass spectrometry-based method for the protein fingerprinting and identification of Aeromonas species using whole cells

Cited by 78 publications

References 33 publications

Detection and diversity of aeromonads from treated wastewater and fish inhabiting effluent and downstream waters

Detection and diversity of aeromonads from treated wastewater and fish inhabiting effluent and downstream waters

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Can Accurately Differentiate Aeromonas dhakensis from A. hydrophila, A. caviae, and A. veronii

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