The development of a recombinant Babesia vaccine

Wright, I.G.; Casu, Rosanne E.; Commins, M.A.; Dalrymple, Brian P.; Gale, K. R.; Goodger, B. V.; Riddles, Peter W.; Waltisbuhl, D.J.; Abetz, I.; Berrie, D.A.; Bowles, Y.; Dimmock, Christine M.; Hayes, Traci L.; Kalnins, H.; Leatch, G.; McCrae, R.; Montague, Peter E.; Nisbet, Ian T.; Parrodi, F.; Peters, Jennifer M.; Scheiwe, P.C.; Smith, W.D.; Rode-Bramanis, K.; White, Melissa

doi:10.1016/0304-4017(92)90138-y

Cited by 106 publications

(86 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, cattle immunized with DNA vaccine were also protected against challenge infection although specific antibodies were not detected. Similar observation was also taken in bovine babesiosis in which there was little correlation between antibody titers and protective immunity in animals immunized with parasite antigens or animals that had recovered from infection [16,21,23,24]. It is reported that proliferative responses of peripheral blood mononuclear cells (PBMC) against purified piroplasm of T. sergenti were detected in infected animals [25].…”

supporting

confidence: 60%

Epitope-Mapping of Antigen-Specific T Lymphocyte in Cattle Immunized with Recombinant Major Piroplasm Surface Protein of Theileria sergenti.

Kakuda

Sugimoto

Onuma

2001

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. The cellular immune responses against major piroplasm surface protein (MPSP) of Theileria sergenti were characterized. Three cattle were immunized with recombinant MPSP (C type) encapsulated by mannan-coated liposome. The proliferative responses of peripheral blood mononuclear cells (PBMC) against MPSP were detected in all immunized animals. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that T cell lines derived from each animal expressed relatively high levels of interferon (IFN)-γ mRNA, low levels of interleukin (IL)-2, IL-10, and tumor necrosis factor (TNF)-α mRNAs, but no detectable level of IL-4 mRNA. From the results of T cell epitope-mapping, T-cell lines from two animals responded to DTSKFTPTVAHRLKHAEDLF (residues 61 to 80), while other animal responded to GTGKVYDFVGNFKVTKVKFE (residues 141 to 160). The MPSP-type specific response of a Tcell line was observed in the latter region of MPSP. These data suggest that immunization with cocktail vaccine consisting of different types of MPSP may be necessary in the field trial. KEY WORDS: epitope-mapping, T-cell line, Theileria sergenti.

show abstract

supporting

confidence: 60%

Epitope-Mapping of Antigen-Specific T Lymphocyte in Cattle Immunized with Recombinant Major Piroplasm Surface Protein of Theileria sergenti.

Kakuda

Sugimoto

Onuma

2001

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…has been slow. Immunization with native or recombinant rhoptry-associated proteins (RAP-1), which are targeted immunogens for vaccine development of B. bovis and B bigemina, reduced parasitemia in infected cattle [40,42]. Immunization with the N-terminal region containing immuno-dominant T-cell epitopes of B. bovis imparted no protection, despite specific RAP-1 immunoglobulin G and CD4 + T-cell responses to challenge [41].…”

Section: Protective Immune Mechanismsmentioning

confidence: 99%

“…Immunization with the N-terminal region containing immuno-dominant T-cell epitopes of B. bovis imparted no protection, despite specific RAP-1 immunoglobulin G and CD4 + T-cell responses to challenge [41]. Because immuno-dominant antigens eliciting strong cellmediated immune responses failed to confer protective immunity, it has been suggested that identification of subdominant antigens might be important for an effective vaccine [39][40][41][42]. The annotation of the B. bovis genome sequence should enable effective antigens to be identified.…”

Section: Protective Immune Mechanismsmentioning

confidence: 99%

“…The annotation of the B. bovis genome sequence should enable effective antigens to be identified. A protective vaccine might need to include multiple antigens [39] and its formulation would require a better understanding of antigenic diversity, mechanisms used by Babesia to evade host immunity and the heterogeneity of the bovine major histocompatibility complex class II molecules [40,[42][43][44].…”

Section: Protective Immune Mechanismsmentioning

confidence: 99%

See 1 more Smart Citation

Attenuated vaccines for tropical theileriosis, babesiosis and heartwater: the continuing necessity

Shkap¹,

Vos²,

Zweygarth³

et al. 2007

Trends in Parasitology

View full text Add to dashboard Cite

“…In the last decade, vaccination studies have focused on Babesia subunit antigens , Wright et al 1992. This strategy has some advantages, such as the low antigen concentration necessary for immune stimulation, no adverse reaction and production of large amounts of antigen by recombinant DNA technology (Wright et al 1992).…”

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 99%

Genetic and antigenic analysis of Babesia bigemina isolates from five geographical regions of Brazil

et al. 2002

View full text Add to dashboard Cite

A molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surface antigens, major surface antigen (MSA)-1 and MSA-2 showed antigenic diversity. However, B cell epitopes on rhoptries and infected erythrocytes were conserved among all isolates studied. In this study it was possible to identify variable and conserved antigens, which had already been described as potential immunogens. Considering that an attenuated Babesia clone used as immunogen selected populations capable of evading the immunity induced by this vaccine, it is necessary to evaluate more deeply the cross-protection conferred by genetically more distant Brazilian B. bigemina isolates and make an evaluation of the polymorphism degree of variable antigens such as MSA-1 and MSA-2.INDEX TERMS: Babesia bigemina, Brazilian isolates, genetic polymorphism, antigenic polymorphism.

show abstract

The development of a recombinant Babesia vaccine

Cited by 106 publications

References 23 publications

Epitope-Mapping of Antigen-Specific T Lymphocyte in Cattle Immunized with Recombinant Major Piroplasm Surface Protein of Theileria sergenti.

Epitope-Mapping of Antigen-Specific T Lymphocyte in Cattle Immunized with Recombinant Major Piroplasm Surface Protein of Theileria sergenti.

Attenuated vaccines for tropical theileriosis, babesiosis and heartwater: the continuing necessity

Genetic and antigenic analysis of Babesia bigemina isolates from five geographical regions of Brazil

Contact Info

Product

Resources

About