The development of an evaluation method for capture columns used in two-dimensional liquid chromatography

Cao, Liwei; Yu, Danhua; Wang, Xinliang; Ke, Yanxiong; Jin, Yu; Liang, Xinmiao

doi:10.1016/j.aca.2011.08.009

Cited by 16 publications

(4 citation statements)

References 27 publications

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“…For 2 D separation, the Hypersil ODS‐2 C 18 column showed better separation performance for the seven additives. Due to the dilution effect of 1 D, a trapping column was used as the interface between 1 D and 2 D, which should result in better enrichment of the targets .…”

Section: Resultsmentioning

confidence: 99%

Simultaneous quantification of five proteins and seven additives in dairy products with a heart-cutting two-dimensional liquid chromatography method

Hou

Sun

et al. 2015

J. Sep. Science

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A heart-cutting two-dimensional high-performance liquid chromatography method was developed to simultaneously quantify five major proteins and seven food additives (maltol, ethyl maltol, vanillin, ethyl vanillin, benzoic acid, sorbic acid, and saccharin sodium) in milk and milk powders. In this two-dimensional system, a Venusil XBP-C4 column was selected in the first dimension for protein separation, and a Hypersil ODS-2 C18 column was employed in the second dimension for additive separation; a two-position, six-port switching valve was used to transfer the targets (additives) from the first dimension to the second dimension. Method validation consisted of selectivity, response function, linearity, precision, sensitivity, and recovery. In addition, a conventional one-dimensional high-performance liquid chromatography method was also tested for comparison. The two-dimensional method resulted in significantly improved recovery of the food additives compared to the conventional method (90.6-105.4% and 65.5-86.5%, respectively). Furthermore, this novel method has a simple one-step sample preparation procedure, which shortens the analysis time, resulting in more efficient analysis and less solvent usage.

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Section: Resultsmentioning

confidence: 99%

Simultaneous quantification of five proteins and seven additives in dairy products with a heart-cutting two-dimensional liquid chromatography method

Hou

Sun

et al. 2015

J. Sep. Science

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show abstract

“…Then, the two-position eight-port valve was automatically switched to the second separation mode. The 18 trap columns were eluted with 15,16,16,19,20,19,25,23,24,29,30,30,30,34,34,35,43, and 43% acetonitrile, respectively. Their elution time was 30, 30, 30, 40, 60, 60, 40, 90, 70, 60, 60, 60, 60 60, 60, 90, 40, and 60 min, respectively.…”

Section: Online Prep-hplc Total Operation Proceduresmentioning

confidence: 99%

“…Because the trap columns unit of the online prep-HPLC system is a very important component, the sample should be retained well on the trap columns, and the target compounds retained on the trap columns should be easily eluted [19]. In this study, each eluent fraction from the first separation was used to evaluate the performance and selection of the trap columns.…”

Section: Evaluation and Selection Of The Trap Columnmentioning

confidence: 99%

A novel online preparative high‐performance liquid chromatography system with the multiple trap columns‐valve switch technique for the rapid and efficient isolation of main flavonoids from Epimedium koreanum Nakai

Zhuang

Ding

et al. 2020

J of Separation Science

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In this work, a new online preparative high‐performance liquid chromatography was developed for the fast and efficient separation of complex chemical mixtures from natural products. This system integrates two chromatographic systems into an online automatic separation system using the technique of multiple trap columns with valve switching. The sample was first separated into 18 subfractions in the online preparative high‐performance liquid chromatography, and the sample eluents were then diluted and captured online on 18 trap columns by the multiple trap columns technique, respectively. Each subfraction retained on the trap column was transferred online to the separation column for the second separation. Finally, the target compounds were purified by appropriate separation conditions and multiple heart‐cutting strategies. Importantly, the system was successfully used to separate 18 high‐purity flavonoids from the crude extract of Epimedium koreanum Nakai online in one step. The entire separation time was approximately 20 h, and the structures were characterized by the high‐performance liquid chromatography quadrupole time‐of‐flight tandem mass spectrometry and nuclear magnetic resonance. This online preparative high‐performance liquid chromatography system represents an efficient and rapid separation system that has the potential for a wide array of applications in the separation of complex chemical components from natural products.

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“…For 2D separation, Hypersil ODS-2 C18 column showed better separation performance for the seven additives. A trapping column was used as the interface between 1D and 2D, which should result in better enrichment of the targets [16]. For online 2DLC, the choice of the mobile phase is very important.…”

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

Analysis of Additives in Milk Powders with SPE-HPLC or 2D-HPLC Method

Hou¹,

Ma²,

Liang³

et al. 2019

Milk Production, Processing and Marketing

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Dairy products are beneficial to human health, especially for formula-fed newborns. According to the regulation of FDA and China national food safety standard, food additives such as benzoic acid, sorbic acid, natamycin, lysozyme, saccharin sodium, and aspartame are not permitted to be added to milk powder. So, the establishment of accurate and convenient methods for the analysis of these food additives in milk powder is critical to people's health. For the reason of the complex matrix of infant milk powders, we compared six sample pretreatment methods (liquid-liquid extraction, organic precipitation, heavy precipitation, and three different solid-phase extraction (SPE) methods (C18, HLB, MAX)) from recovery, easy operation, time cost, and organic solvent usage aspects. Finally, Poly-Sery HLB cartridge was confirmed as the most appropriate material for its high recovery and time cost merits. We are also introducing two-dimensional liquid chromatography (2DLC) method for the simultaneous determination of five major proteins and seven food additives in milk powders. Optimization of switching mode, choice of columns, mobile phase, and flow speed was discussed. We also compared limit of detection (LOD), recovery, and sample treatment with the results of high-performance liquid chromatography (HPLC). Results show that 2DLC is simpler, faster, and more accurate than the HPLC method.

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The development of an evaluation method for capture columns used in two-dimensional liquid chromatography

Cited by 16 publications

References 27 publications

Simultaneous quantification of five proteins and seven additives in dairy products with a heart-cutting two-dimensional liquid chromatography method

Simultaneous quantification of five proteins and seven additives in dairy products with a heart-cutting two-dimensional liquid chromatography method

A novel online preparative high‐performance liquid chromatography system with the multiple trap columns‐valve switch technique for the rapid and efficient isolation of main flavonoids from Epimedium koreanum Nakai

Analysis of Additives in Milk Powders with SPE-HPLC or 2D-HPLC Method

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