The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato

Garland, SM; Sharman, Murray; Persley, D. M.; McGrath, D. J.

doi:10.1071/ar04140

Cited by 29 publications

(15 citation statements)

References 15 publications

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“…The tospovirus resistance protein A of the gene Sw5-a is complement to the sequence of the Sw-5 locus located at AY007366 from 20824 to 24561 base and the tospovirus resistance protein B of the gene Sw5-b is complement to the sequence of the Sw-5 locus located at AY007366 from 29513 to 33253 base (Folkertsma et al1999, GenBank Accession AY007366). Garland et al (2005) reported a dominant PCR-based marker representing the Sw-5 gene sequence co-segregated with the RFLP marker CT 220 (Stevens et al 1995(Stevens et al , 1996 in 50 individuals of an F 2 mapping population.…”

Section: Introductionmentioning

confidence: 99%

Identification of molecular markers for Sw-5 gene of tomato spotted wilt virus resistance

Shi¹,

Vierling²

2011

AJBMS

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Tomato spotted wilt virus (TSWV) is one of the most destructive viral diseases that threaten worldwide tomato (Solanuum lycopersicum L. syn Lycopersicon esculentum Mill.) production. Use of host resistance appears to be the best way to control the disease. Marker assisted selection (MAS) has become very important and useful in the selection of TSWV resistance genes. The objective of this research was to identify Sw-5 gene-specific PCR-based molecular markers and gene-derived single nucleotide polymorphism (SNP) markers for MAS in tomato breeding. A gene-specific PCR-based marker and two gene-derived SNP markers specific for TSWV resistance Sw5-b allele were identified and validated in different tomato germplasm. It is expected that the present findings will be helpful in advancing the MAS for TSWV resistance in tomato.

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Section: Introductionmentioning

confidence: 99%

Identification of molecular markers for Sw-5 gene of tomato spotted wilt virus resistance

Shi¹,

Vierling²

2011

AJBMS

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“…In this context, the 'Sw-5-2' co-dominant, within-locus, SCAR marker system is more advantageous than the other closely linked markers reported thus far. In addition, the SCAR marker reported here is much simpler than the CAPS marker system reported by Garland et al (2005), which requires the use of the closely linked CT220 marker as a positive control in order to discriminate susceptible plants from false negative PCR reactions (Garland et al 2005). It is important to mention that the amount of sequence variation observed in the genomic region encompassing this polymorphic amplicon might allow the further development of even better markers potentially suitable for gel-free systems.…”

Section: Resultsmentioning

confidence: 99%

“…These are closely linked markers, which might occasionally be separated from the gene of interest through genetic recombination (crossing-over) events. In this scenario, markers derived from the resistant locus itself, capable of differentiating among susceptible and resistant plants, would be the ideal ones (Folkertsma et al 1999;Garland et al 2005). Although sequences of the Sw-5 locus are available at public databases, there are few reports of molecular markers derived from them.…”

Section: Introductionmentioning

confidence: 99%

“…Although sequences of the Sw-5 locus are available at public databases, there are few reports of molecular markers derived from them. The work reported by Garland et al (2005) is one of the few exceptions, where a dominant marker derived from the Sw-5 locus combined with a closely linked CAPS marker were employed to fingerprint Tospovirus susceptible and resistant lines.…”

Section: Introductionmentioning

confidence: 99%

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Development of a locus-specific, co-dominant SCAR marker for assisted-selection of the Sw-5 (Tospovirus resistance) gene cluster in a wide range of tomato accessions

Dianese

Fonseca²,

Goldbach

et al. 2009

Mol Breeding

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The best levels of broad-spectrum Tospovirus resistance reported in tomatoes thus far are conferred by the Sw-5 locus. This locus contains at least five paralogues (denoted Sw-5a through Sw-5e), of which Sw-5b represents the actual resistance gene. Here we evaluated a panel of seven PCR primer pairs matching different sequences within a genomic region spanning the Sw-5a and Sw-5b gene cluster. Primer efficiency evaluation was done employing tomato isolines with and without the Sw-5 locus. One primer pair produced a single and co-dominant polymorphism between susceptible and resistant isolines. Sequence analysis of these amplicons indicated that they were specific for the Sw-5 locus and their differences were due to insertions/deletions. The polymorphic SCAR amplicon encompass a conserved sequence of the promoter region of the functional Sw-5b gene, being located in the position -31 from its open reading frame. This primer pair was also evaluated in field assays and with a collection of accessions known to be either susceptible or resistant to tospoviruses. An almost complete correlation was found between resistance under greenhouse/field conditions and the presence of the marker. Therefore, this primer pair is a very useful tool in marker-assisted selection systems in a large range of tomato accessions.

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“…In particular, for TSWV resistance conferred by the Sw-5b gene, diverse molecular markers such as RAPD (random amplified polymorphic DNA), RFLP, SCAR (sequence characterized amplified regions), CAPS, and INDEL markers have been reported (Dianese et al, 2010;Garland et al, 2005;Langella et al, 2004;Roselló et al, 2001;Śmiech et al, 2000;Stevens et al, 1995). Since the successful cloning of the Sw-5b gene, a gene-based marker and two SNPs specific to the Sw-5b allele for TSWV resistance have been reported (Shi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Development of a Single-nucleotide Polymorphism Marker for the Sw-5b Gene Conferring Disease Resistance to Tomato spotted wilt virus in Tomato

Lee¹,

Kim²,

Bae³

et al. 2015

HST

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Tomato spotted wilt virus (TSWV) causes one of the most destructive viral diseases that threatens global tomato production. Sw-5b was reported as the resistance gene effective against TSWV. The objective of this research was to develop a single-nucleotide polymorphism (SNP) marker to distinguish tomato cultivars resistant to TSWV from susceptible cultivars for marker-assisted breeding. First, we determined genotypes for TSWV resistance in 32 commercial tomato cultivars using the previously reported Sw-5b gene-based marker. Then, DNA sequences of Sw-5b alleles in tomato cultivars showing resistant or susceptible genotypes were analyzed; a single SNP was found to distinguish tomato cultivars resistant to TSWV from susceptible cultivars. Based on the confirmed SNP, a SNP primer pair was designed. Using this new SNP sequence and high-resolution melting analysis, the same 32 tomato cultivars were screened. The results were perfectly correlated with those from screening with the Sw-5b gene-based marker. These results indicate that the SNP maker developed in this study will be useful for better tracking of resistance to TSWV in tomato breeding.

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The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato

Cited by 29 publications

References 15 publications

Identification of molecular markers for Sw-5 gene of tomato spotted wilt virus resistance

Identification of molecular markers for Sw-5 gene of tomato spotted wilt virus resistance

Development of a locus-specific, co-dominant SCAR marker for assisted-selection of the Sw-5 (Tospovirus resistance) gene cluster in a wide range of tomato accessions

Development of a Single-nucleotide Polymorphism Marker for the Sw-5b Gene Conferring Disease Resistance to Tomato spotted wilt virus in Tomato

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