The development of an optimized sample preparation for trace level detection of 17α-ethinylestradiol and estrone in whole fish tissue

Al-Ansari, Ahmed M.; Saleem, Ammar; Kimpe, Linda E.; Trudeau, Vance L.; Blais, Jules M.

doi:10.1016/j.jchromb.2011.09.033

Cited by 27 publications

(20 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, the determination of estrogens in milk and dairy products appears as a useful tool to monitor the introduction of natural and synthetic estrogens in the milk cycle by human action [32]. In order to assess the environmental fate of estrogens, several studies have focused on the analysis of fish tissues and plasma samples [33][34][35]. The main purpose of those studies has been to determine the uptake, elimination and bioaccumulation of estrogens, namely EE2, in fish or other aquatic organisms from natural ecosystems exposed to these compounds.…”

Section: Determination Of E2 and Ee2 In Biological Samplesmentioning

confidence: 99%

“…Biological solid samples such as human and animal tissues (including invertebrates) are complex matrices that, prior to extraction, require homogenization with organic solvents [35,41] or buffer solutions [27]. After this step, the resultant homogenates are often freeze-dried, as this procedure has been associated with higher steroidal estrogens recoveries [34,35]. Homogenates extraction may then be performed using different techniques.…”

Section: Sample Preparationmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of 17-β-estradiol and 17-α-ethinylestradiol in biological and environmental matrices — A review

Barreiros

Queiroz

Magalhães

et al. 2016

Microchemical Journal

125

View full text Add to dashboard Cite

The estrogens 17-β-estradiol (E2) and 17-α-ethinylestradiol (EE2) are reported as highly endocrine-disrupting agents, being recently included in an EU watch list regarding emerging aquatic pollutants. Therefore, the monitoring of these chemicals in the different environmental compartments assumes great importance. Moreover, due to the possible adverse effects on living beings, their occurrence on animal tissues and fluids must also be addressed. In recent years, a significant number of studies have described and proposed different analytical methodologies to detect and/or quantify E2 and EE2 mostly in environmental aqueous samples, including sludge and sediments and also in biological matrices such as plasma and tissues. Taking into account the complexity of real matrices and that both estrogens are generally present at trace levels, the development of accurate and reliable techniques for their determination can be quite a challenge. The present review aims at describing the main characteristics of the analytical methods recently used for E2 and EE2 determination in environmental and biological samples. The steps for sample preparation such as analytes extraction, preconcentration and clean-up are discussed and the instrumental based analytical techniques are compared. Furthermore, the application of biological tools to determine the total estrogenicity of environmental samples, as well as their potential combination with instrumental analyses, is highlighted.

show abstract

Section: Determination Of E2 and Ee2 In Biological Samplesmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Analysis of 17-β-estradiol and 17-α-ethinylestradiol in biological and environmental matrices — A review

Barreiros

Queiroz

Magalhães

et al. 2016

Microchemical Journal

125

View full text Add to dashboard Cite

show abstract

“…Beef patties were pan‐cooked as described for tofu patties and dry matter was determined in quintuplicate on cooked samples (Table S4). Cooked burgers were extracted in a modification of a method for extraction of estrogens from fish (Al‐Ansari, Saleem, Kimpe, Trudeau, & Blias, ). A portion of the crumbled patty (5 g postcooking weight) was mixed with approximately 2 g of solvent‐extracted diatomaceous earth (DE extracted on an Accelerated Solvent Extractor 200 [ASE], Dionex Corp., Salt Lake City, UT, USA).…”

Section: Methodsmentioning

confidence: 99%

An In Vitro Comparison of Estrogenic Equivalents Per Serving Size of Some Common Foods

Shappell

Berg

Magolski

et al. 2019

Journal of Food Science

View full text Add to dashboard Cite

The public assumes that some foods, such as milk and ground beef from cattle receiving steroidal implants, are associated with estrogenic hormones, while other foods are presumed "safe" or nonestrogenic. Here, we investigate these assumptions by assessing the relative estrogenic activity of a serving size of four foods: skim milk (8 oz), rice (48 g dry wt) in cooking bag, ground beef patties from steers raised with or without hormone implantation (quarter lb each, 114 g), and tofu burgers (isocaloric to beef burger, 198 g), using an in vitro assay (E-Screen). Mean picogram (pg) estradiol equivalents (E2Eqs) on a serving basis were as follows: skim milk 120; rice 400; rice prepared in cooking bag 370; rice boiling bag alone 4 pg per bag, ground beef burger (obtained from the tissue of cattle that had received no hormone implants) 389, beef burger (obtained from cattle that had received hormone implant) 384, and tofu burger 1,020,000. Rice E2Eqs were highly variable, but the plastic cooking bags provided by the manufacturer added negligible E2Eqs. The source of estrogenic activity in rice may have been due to contamination with the mycotoxin zearalenone. The E-Screen E2Eqs of tofu burger extracts agreed with those predicted based on chemical concentrations of the most estrogenic component times their E2Eq factor. While a tofu burger contained around three times the estrogenic activity of a daily dose of estrogen replacement therapy (125 mg, Premarin R , 303,000 pg); the other foods--a quarter pound ground beef burger at approximately equal calorie count, a serving of milk, or rice, were all at least 750-fold less estrogenic.Practical Application: When consuming the recognized serving size of a food, how much estrogenic activity can we expect? While the public assumes that some foods, such as milk and ground beef from cattle receiving steroidal implants, are associated with estrogenic hormones, other foods are presumed "safe" or nonestrogenic. Using one assay, a tofu burger contained three times the estrogenic activity of a dose of hormone replacement therapy commonly prescribed for women after hysterectomy or menopause (Premarin R ); while other foods--a quarter pound ground beef burger at approximately equal calorie count, a serving of milk, or rice, were all at least 750-fold less estrogenic.

show abstract

“…Cooked ground beef burgers were extracted in a modification of a method for extraction of estrogens from fish (11). Samples were extracted with acetone using an accelerated solvent extractor (ASE 200, Dionex; Dionex Corporation) followed by liquid extraction with hexane and volatilization of solvent (see online supplementary material) and resuspended to 100 aqueous mL for solid phase extraction as described previously.…”

Section: Estrogenicity Assaymentioning

confidence: 99%

Consumption of Ground Beef Obtained from Cattle That Had Received Steroidal Growth Promotants Does Not Trigger Early Onset of Estrus in Prepubertal Pigs

Magolski

Shappell

Vonnahme

et al. 2014

The Journal of Nutrition

View full text Add to dashboard Cite

show abstract

The development of an optimized sample preparation for trace level detection of 17α-ethinylestradiol and estrone in whole fish tissue

Cited by 27 publications

References 17 publications

Analysis of 17-β-estradiol and 17-α-ethinylestradiol in biological and environmental matrices — A review

Analysis of 17-β-estradiol and 17-α-ethinylestradiol in biological and environmental matrices — A review

An In Vitro Comparison of Estrogenic Equivalents Per Serving Size of Some Common Foods

Consumption of Ground Beef Obtained from Cattle That Had Received Steroidal Growth Promotants Does Not Trigger Early Onset of Estrus in Prepubertal Pigs

Contact Info

Product

Resources

About