The development of behavioral sensitization induced by a single morphine exposure in adult zebrafish (Danio rerio)

Bian, Yaqi; Wang, Xiufen; Liang, Jing; Lin, Liang-In; Wu, Xue; Tang, Ben-Qin; Leung, George Pak‐Heng; Lee, Simon Ming‐Yuen

doi:10.1016/j.pnpbp.2021.110456

Cited by 4 publications

(1 citation statement)

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“…Zebrafish exhibit stronger behavioral responses after repeated exposure to cocaine, nicotine, and ethanol, which is consistent with our findings ( Pisera-Fuster et al, 2019 ). In adult zebrafish, behavioral sensitization generated by a single morphine exposure resulted in significant behavioral sensitization after repeated and intermittent morphine (1.25–10 µM, 0.36−2.85 mg/L) treatment ( Bian et al, 2022 ). The dosages employed to elicit behavioral sensitization in zebrafish are typically low, owing to the fact that excessive concentrations produce a decrease in locomotor activity.…”

Section: Discussionmentioning

confidence: 99%

Embryonic exposure to fentanyl induces behavioral changes and neurotoxicity in zebrafish larvae

Wang

Chen

Zhong

et al. 2022

PeerJ

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The use of fentanyl during pregnancy, whether by prescription or illicit use, may result in high blood levels that pose an early risk to fetal development. However, little is known regarding the neurotoxicity that might arise from excessive fentanyl exposure in growing organisms, particularly drug-related withdrawal symptoms. In this study, zebrafish embryos were exposed to fentanyl solutions (0.1, 1, and 5 mg/L) for 5 days post fertilization (dpf), followed by a 5-day recovery period, and then the larvae were evaluated for photomotor response, anxiety behavior, shoaling behavior, aggression, social preference, and sensitization behavior. Fentanyl solutions at 1 and 5 mg/L induced elevated anxiety, decreased social preference and aggressiveness, and behavioral sensitization in zebrafish larvae. The expression of genes revealed that embryonic exposure to fentanyl caused substantial alterations in neural activity (bdnf, c-fos) and neuronal development and plasticity (npas4a, egr1, btg2, ier2a, vgf). These results suggest that fentanyl exposure during embryonic development is neurotoxic, highlighting the importance of zebrafish as an aquatic species in research on the neurobehavioral effects of opioids in vertebrates.

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Section: Discussionmentioning

confidence: 99%