The Development of Electrochemical Aptasensor Based on DNA Aptamers Modified by Redox Markers for Detection of Leukemia Jurkat Cells

Babelova, Lenka; Slabý, Cyril; Hianik, Tibor

doi:10.3390/iecb2020-07082

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…For acute lymphoblastic leukemia, an oncological disease prevalent in children, a redox-labeled electrochemical aptasensor that specifically detects the cancer biomarker protein tyrosine kinase 7 (PTK7) was developed [153]. Detection was performed using Jurkat leukemia cells that overexpress PTK7, and for this purpose, two aptasensors with different redox markers (MB or Fc carboxylic acid) were described.…”

Section: Sassolas Et Al Reported That Amperometric and Impedimetric M...mentioning

confidence: 99%

Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications

et al. 2023

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Many biological processes (physiological or pathological) are relevant to membrane proteins (MPs), which account for almost 30% of the total of human proteins. As such, MPs can serve as predictive molecular biomarkers for disease diagnosis and prognosis. Indeed, cell surface MPs are an important class of attractive targets of the currently prescribed therapeutic drugs and diagnostic molecules used in disease detection. The oligonucleotides known as aptamers can be selected against a particular target with high affinity and selectivity by iterative rounds of in vitro library evolution, known as Systematic Evolution of Ligands by EXponential Enrichment (SELEX). As an alternative to antibodies, aptamers offer unique features like thermal stability, low-cost, reuse, ease of chemical modification, and compatibility with various detection techniques. Particularly, immobilized-aptamer sensing platforms have been under investigation for diagnostics and have demonstrated significant value compared to other analytical techniques. These “aptasensors” can be classified into several types based on their working principle, which are commonly electrochemical, optical, or mass-sensitive. In this review, we review the studies on aptamer-based MP-sensing technologies for diagnostic applications and have included new methodological variations undertaken in recent years.

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Section: Sassolas Et Al Reported That Amperometric and Impedimetric M...mentioning

confidence: 99%

Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications

et al. 2023

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“…This approach has been used for the detection of Jurkat cells by sgc8c aptamers modified at the 3 end by ferrocene (Fc) or methylene blue (MB). The aptamers were immobilized on the gold surface by chemisorption or biotinneutravidin methods [103,106]. The redox potential for these probes is different.…”

Section: Electrochemical and Acoustic Aptasensors 331 Electrochemical Aptasensors For Detection Leukemia Cellsmentioning

confidence: 99%

Advances in Electrochemical and Acoustic Aptamer-Based Biosensors and Immunosensors in Diagnostics of Leukemia

Hianik¹

2021

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Early diagnostics of leukemia is crucial for successful therapy of this disease. Therefore, development of rapid, sensitive, and easy-to-use methods for detection of this disease is of increased interest. Biosensor technology is challenged for this purpose. This review includes a brief description of the methods used in current clinical diagnostics of leukemia and provides recent achievements in sensor technology based on immuno- and DNA aptamer-based electrochemical and acoustic biosensors. The comparative analysis of immuno- and aptamer-based sensors shows a significant advantage of DNA aptasensors over immunosensors in the detection of cancer cells. The acoustic technique is of comparable sensitivity with those based on electrochemical methods; moreover, it is label-free and provides straightforward evaluation of the signal. Several examples of sensor development are provided and discussed.

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“…A nanostructure is a form of electrode that has the potential to become a biosensor due to its high sensitivity and accuracy in detecting cancer biomarkers and its nature attractiveness with molecules [19]. Gold (Au) is commonly used as a biosensor [20][21][22]. However, Ag is also a good candidate for biosensors.…”

Section: Introductionmentioning

confidence: 99%

A Comparison between Silver Nanosquare Arrays and Silver Thin-Films as a Blood Cancer Prognosis Monitoring Electrode Design Using Optical and Electrochemical Characterization

et al. 2021

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The development of silver (Ag) thin films and the fabrication of Ag nanosquare arrays with the use of an anodic aluminum oxide (AAO) template and leaf extracts were successfully carried out using the DC sputtering and spin coating deposition methods. Ag thin films and Ag nanosquare arrays are developed to monitor cancer prognosis due to the correlation between serum albumin levels and prognostic factors, as well as the binding of serum albumin to the surface of these electrodes. Nanosquare structures were fabricated using AAO templates with varying diameters and a gap distance between adjacent unit cells of 100 nm. The nanosquare array with a diameter of 250 nm and irradiated with electromagnetic waves with a wavelength of around 800 nm possessed the greatest electric field distribution compared to the other variations of diameters and wavelengths. The results of the absorption measurement and simulation showed a greater shift in absorption peak wavelength when carried out using the Ag nanosquare array. The absorption peak wavelengths of the Ag nanosquare array in normal blood and blood with cancer lymphocytes were 700–774 nm and 800–850 nm, respectively. The electrochemical test showed that the sensitivity values of the Ag thin-film electrode deposited using DC sputtering, the Ag thin-film electrode deposited using spin coating, and the Ag nanosquare array in detecting PBS+BSA concentration in the cyclic voltammetry (CV) experiment were 1.308 µA mM−1cm−2, 0.022 µA mM−1cm−2, and 39.917 µA mM−1cm−2, respectively. Meanwhile, the sensitivity values of the Ag thin film and the Ag nanosquare array in detecting the PBS+BSA concentration in the electrochemical impedance spectroscopy (EIS) measurement were 6593.76 Ohm·cm2/mM and 69,000 Ohm·cm2/mM, respectively. Thus, our analysis of the optical and electrochemical characteristics of Ag thin films and Ag nanosquare arrays showed that both can be used as an alternative biomedical technology to monitor the prognosis of blood cancer based on the concentration of serum albumin in blood.

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The Development of Electrochemical Aptasensor Based on DNA Aptamers Modified by Redox Markers for Detection of Leukemia Jurkat Cells

Cited by 3 publications

References 20 publications

Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications

Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications

Advances in Electrochemical and Acoustic Aptamer-Based Biosensors and Immunosensors in Diagnostics of Leukemia

A Comparison between Silver Nanosquare Arrays and Silver Thin-Films as a Blood Cancer Prognosis Monitoring Electrode Design Using Optical and Electrochemical Characterization

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