The DI-SPME Method for Determination of Selected Narcotics and Their Metabolites, and Application to Bone Marrow and Whole Blood Analysis

Świądro, Magdalena; Chromiec, Alicja; Zawadzki, Marcin; Wietecha‐Posłuszny, Renata

doi:10.3390/molecules27134116

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…Also, it considerably reduces the possibility of the decomposition and loss of heat-sensitive compounds during the extraction. This technique is used in a wide variety of fields, ranging from food ( Mohammadhosseini et al, 2017 ), environment ( Conrady et al, 2021 ), agriculture ( Mohammadhosseini, 2015b ), and medicine ( Świądro-Piętoń et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Antifungal activity of the volatile organic compounds produced by Ceratocystis fimbriata strains WSJK-1 and Mby

Gao

Ren²,

ShuQi³

et al. 2022

Front. Microbiol.

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Microorganism-produced volatile organic compounds (VOCs) are considered promising environmental-safety fumigants in food preservation. In this study, the VOCs from fungal Ceratocystis fimbriata strains (WSJK-1, Mby) were tested against postharvest fungi Monilinia laxa, Fusarium oxysporum, Monilinia fructicola, Botrytis cinerea, Alternaria solani, and Aspergillus flavus in vitro. The mycelial growth was significantly inhibited, in particular M. fructicola and B. cinerea (76.95, 76.00%), respectively. VOCs were identified by headspace solid-phase microextraction coupled with Gas Chromatography–Mass Spectrometry (HS-SPME-GC–MS); 40 compounds were identified. The antifungal activity of 21 compounds was tested by the minimum inhibitory concentrations (MIC) value. Benzaldehyde, 2-Phenylethanol, and 1-Octen-3-ol showed strong antifungal activity with the MIC in vitro ranging from 0.094 to 0.284 ml L−1 depending on the pathogen tested. The optical microscope showed serious morphological damage, including cell deformation, curling, collapse, and deficiency in mycelial or conidia cell structures treated with C. fimbriata VOCs and pure compounds. In vivo tests, C. fimbriata VOCs decreased brown rot severity in peaches, and compounds Benzaldehyde and 2-Phenylethanol could reduce peach brown rot in peaches at 60 μl L−1. The VOCs produced by C. fimbriata strain have good antifungal effects; low concentration fumigation could control peach brown rot. Its fragrance is fresh, safe, and harmless, and it is possible to replace chemical fumigants. It could be used as a potential biofumigant to control fruit postharvest transportation, storage, and food preservation. To the best of our knowledge, this is the first report on the antifungal activity and biocontrol mechanism of VOCs produced by C. fimbriata.

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Section: Introductionmentioning

confidence: 99%

Antifungal activity of the volatile organic compounds produced by Ceratocystis fimbriata strains WSJK-1 and Mby

Gao

Ren²,

ShuQi³

et al. 2022

Front. Microbiol.

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New green DI-SPME/CE-MS method for quantitative analysis of selected illicit drugs in human whole blood

Chromiec

Ordon

Wietecha‐Posłuszny

2023

Green Analytical Chemistry

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Determination of Amphetamines, Opioids, Cocaine Metabolites and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol in Urine Using Biocompatible Solid-Phase Microextraction and Ultra-Performance LC–MS/MS

Schneider,

Lima Feltraco Lizot,

Reginatto

et al. 2023

Bioanalysis

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Background: Workplace drug testing primarily relies on urine analysis, targeting multiple compounds with varying physicochemical characteristics. Biocompatible solid-phase microextraction (BioSPME) is a miniaturized solid-phase extraction technique that enables the simultaneous extraction and preconcentration of analytes directly from the biological matrix. Methods: The BioSPME procedure consisted of the sequential extraction of 50-μl urine samples using LC Tips C18 in basic and acidic pH, followed by desorption with methanol and n-hexane, respectively. The extracts were analyzed by ultra-performance LC–MS/MS. Results: Intra-day precision was 1.2–8.6% and inter-day precision was 1.8–14.2%. Accuracy was 96.8–107.4%. The extraction yields were 62.8–109.4%. The matrix effects were -3.98% to 1%. Conclusion: BioSPME shows promise as an alternative method for preparing urine samples prior to drug measurement by ultra-performance LC–MS/MS.

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The DI-SPME Method for Determination of Selected Narcotics and Their Metabolites, and Application to Bone Marrow and Whole Blood Analysis

Cited by 3 publications

References 27 publications

Antifungal activity of the volatile organic compounds produced by Ceratocystis fimbriata strains WSJK-1 and Mby

Antifungal activity of the volatile organic compounds produced by Ceratocystis fimbriata strains WSJK-1 and Mby

New green DI-SPME/CE-MS method for quantitative analysis of selected illicit drugs in human whole blood

Determination of Amphetamines, Opioids, Cocaine Metabolites and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol in Urine Using Biocompatible Solid-Phase Microextraction and Ultra-Performance LC–MS/MS

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