The Diabetic Lung Can Be Ameliorated by <i>Citrullus colocynthis</i> by Reducing Inflammation and Oxidative Stress in Rats with Type 1 Diabetes

Bejeshk, Mohammad Abbas; Bagheri, Fariborz; Salimi, Fouzieh; Rajizadeh, Mohammad Amin

doi:10.1155/2023/5176645

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…Also, Dennis et al reported that increased TNFα levels are associated with inadequate glucose control in T2D and impaired lung function [ 41 ]. In another study, it has been shown that the levels of IL-6 and IL-17 were increased in diabetic lungs [ 42 ]. Diabetes is a pro-inflammatory state associated with airway inflammation [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Lung molecular and histological changes in type 2 diabetic rats and its improvement by high-intensity interval training

Rajizadeh,

Khoramipour,

Joukar

et al. 2024

BMC Pulm Med

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Background Type 2 diabetes (T2D) leads to serious respiratory problems. This study investigated the effectiveness of high-intensity interval training (HIIT) on T2D-induced lung injuries at histopathological and molecular levels. Methods Forty-eight male Wistar rats were randomly allocated into control (CTL), Diabetes (Db), exercise (Ex), and Diabetes + exercise (Db + Ex) groups. T2D was induced by a high-fat diet plus (35 mg/kg) of streptozotocin (STZ) administration. Rats in Ex and Db + Ex performed HIIT for eight weeks. Tumor necrosis factor-alpha (TNFα), Interleukin 10 (IL-10), BAX, Bcl2, Lecithin, Sphingomyelin (SPM) and Surfactant protein D (SPD) levels were measured in the bronchoalveolar lavage fluid (BALF) and malondialdehyde (MDA) and total antioxidant capacity (TAC) levels were measured in lung tissue. Lung histopathological alterations were assessed by using H&E and trichrome mason staining. Results Diabetes was significantly associated with imbalance in pro/anti-inflammatory, pro/anti-apoptosis and redox systems, and reduced the SPD, lecithin sphingomyelin and alveolar number. Performing HIIT by diabetic animals increased Bcl2 (P < 0.05) and IL10 (P < 0.01) levels as well as surfactants components and TAC (P < 0.05) but decreased fasting blood glucose (P < 0.001), TNFα (P < 0.05), BAX (P < 0.05) and BAX/Bcl2 (P < 0.001) levels as well as MDA (P < 0.01) and MDA/TAC (P < 0.01) compared to the diabetic group. Furthermore, lung injury and fibrosis scores were increased by T2D and recovered in presence of HIIT. Conclusion These findings suggested that the attenuating effect of HIIT on diabetic lung injury mediated by reducing blood sugar, inflammation, oxidative stress, and apoptosis as well as improving pulmonary surfactants components. Graphical Abstract Type 2 diabetes increased inflammation, oxidative stress and apoptosis and reduced pulmonary surfactants , while high intensity training improved these negative effects

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Section: Discussionmentioning

confidence: 99%

Lung molecular and histological changes in type 2 diabetic rats and its improvement by high-intensity interval training

Rajizadeh,

Khoramipour,

Joukar

et al. 2024

BMC Pulm Med

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“…After euthanizing the animals and removing the heart, the right lung was clamped, and 1 ml of phosphate-buffered saline (PBS) was injected using a syringe through a tracheal cannula into the left lung. Following a 2-minute interval, the infused liquid was lavaged and subsequently stored at − 80 °C [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

High-intensity intermittent training ameliorates methotrexate-induced acute lung injury

Rajizadeh,

Hosseini,

Bahrami

et al. 2024

BMC Pulm Med

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Inflammation and oxidative stress are recognized as two primary causes of lung damage induced by methotrexate, a drug used in the treatment of cancer and immunological diseases. This drug triggers the generation of oxidants, leading to lung injury. Given the antioxidant and anti-inflammatory effects of high-intensity intermittent training (HIIT), our aim was to evaluate the therapeutic potential of HIIT in mitigating methotrexate-induced lung damage in rats. Seventy male Wistar rats were randomly divided into five groups: CTL (Control), HIIT (High-intensity intermittent training), ALI (Acute Lung Injury), HIIT+ALI (pretreated with HIIT), and ALI + HIIT (treated with HIIT).HIIT sessions were conducted for 8 weeks. At the end of the study, assessments were made on malondialdehyde, total antioxidant capacity (TAC), superoxide dismutase (SOD), glutathione peroxidase (Gpx), myeloperoxidase (MPO), interleukin 10 (IL-10), tumor necrosis factor-alpha (TNF-α), gene expression of T-bet, GATA3, FOXP3, lung wet/dry weight ratio, pulmonary capillary permeability, apoptosis (Caspase-3), and histopathological indices.Methotrexate administration resulted in increased levels of TNF-α, MPO, GATA3, caspase-3, and pulmonary edema indices, while reducing the levels of TAC, SOD, Gpx, IL-10, T-bet, and FOXP3. Pretreatment and treatment with HIIT reduced the levels of oxidant and inflammatory factors, pulmonary edema, and other histopathological indicators. Concurrently, HIIT increased the levels of antioxidant and anti-inflammatory factors.

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“…According to the World Health Organization (WHO), the number of diabetics was 171 million in 2000 and is estimated to reach 366 million by 2030 2 . Diabetes is considered a leading risk factor for cardiomyopathy, neuropathy, nephropathy, and retinopathy and represents a significant economic burden for countries worldwide 3 – 5 .…”

Section: Introductionmentioning

confidence: 99%

ATP releasing channels and the ameliorative effects of high intensity interval training on diabetic heart: a multifaceted analysis

Joukar,

Rajizadeh,

Bejeshk

et al. 2024

Sci Rep

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Type 2 diabetes (T2D) can cause severe cardiac complications at functional, histologic and molecular levels. These pathological complications could be mediated by ATP-releasing channels such as Panx1 and ATP receptors, in particular P2X7. The aim of our study was to investigate the effect of high-intensity interval training (HIIT) on T2D-induced cardiac complications at the functional, histopathological and molecular levels, with a particular focus on ATP-releasing channels. 48 male Wistar rats at the age of 8 weeks were randomly allocated into four groups: control (Con), Diabetes (T2D), Training (TR), and Diabetes + Training (T2D + TR). T2D was induced by a high-fat diet plus a low dose (35 mg/kg) of STZ administration. Rats in the TR and T2D + TR groups underwent an 8-weeks training program involving intervals ranging from 80 to 100% of their maximum running speed (Vmax), with 4–10 intervals per session. Protein expression of Interleukin 1β (IL1β), Interleukin 10 (IL-10), Pannexin 1 (Panx1), P2X7R (purinergic P2X receptor 7), NLRP1 (NLR Family Pyrin Domain Containing 1), BAX, and Bcl2 were measured in the heart tissue. Additionally, we assessed heart function, histopathological changes, as well as insulin resistance using the homeostasis model assessment of insulin resistance (HOMA-IR). In contrast to the T2D group, HIIT led to increased protein expression of Bcl2 and IL-10 in the heart. It also resulted in improvements in systolic and diastolic blood pressures, heart rate, ± dp/dt (maximum and minimum changes in left ventricular pressure), while reducing protein expression of IL-1β, Panx1, P2X7R, NLRP1, and BAX levels in the heart. Furthermore, left ventricular diastolic pressure (LVDP) was reduced (P ≤ 0.05). Moreover, heart lesion scores increased with T2D but decreased with HIIT, along with a reduction in fibrosis percentage (P ≤ 0.05). The results of this study suggest that the cardioprotective effects of HIIT on the diabetic heart may be mediated by the modulation of ATP-releasing channels. This modulation may lead to a reduction in inflammation and apoptosis, improve cardiac function, and attenuate cardiac injury and fibrosis.

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The Diabetic Lung Can Be Ameliorated by Citrullus colocynthis by Reducing Inflammation and Oxidative Stress in Rats with Type 1 Diabetes

Cited by 6 publications

References 45 publications

Lung molecular and histological changes in type 2 diabetic rats and its improvement by high-intensity interval training

Lung molecular and histological changes in type 2 diabetic rats and its improvement by high-intensity interval training

High-intensity intermittent training ameliorates methotrexate-induced acute lung injury

ATP releasing channels and the ameliorative effects of high intensity interval training on diabetic heart: a multifaceted analysis

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