“…General methods and equipment used in tolerance experiments are described by Pakker et al (1995) Taylor (1928Taylor ( , 1960, Cheney & Dyer (i974), Richardson (1975), Oliveira Filho (1977, Cordeiro-Marino (i978), van den Hoek et al (1978), Price & John (1979), Norris & Bucher (1982), Hanisak & Blair (1988), Schneider & Searles (199I), Falcao et al (1992); eastern Atlantic, Levring (I974), Lawson & John (1977), Gil-Rodriguez & Afonso-Carrillo (I980), Price & John (1980), Lawson & John (1982), Audiffred & Weisscher (1984), Audiffred & Prud'homme van Reine (1985), Price et al (i986, 1988) Three plants were incubated in a 200 ml Erlenmeyer flask, containing IS0ml of medium, and placed in culture cabinets (i0"2°C) at 5, 8, 10, 12, 15, 18, and 20°C. Plants were transferred to the experimental low temperatures in steps of no more than 3°C per 3 days. After 2, 4 and 8 weeks one plant was removed and gradually brought back to the optimal growth temperature.…”