The diversity of polyprenol pattern in leaves of fruit trees belonging to Rosaceae and Cornaceae.

Abstract

The polyprenol pattern in leaves of fruit trees belonging to the Rosaceae (genera: Prunus, Malus) and Cornaceae (genus: Cornus) families is presented. The content of polyprenyl acetates varied within plant species between 10-50 mg per gram of dry weight. In genus Prunus, Cornus and in representatives of species Malus domestica, a mixture of polyprenols composed of 18, 19, 20, 21 isoprene units was found. In six species of genus Prunus (sour-cherry): P. serrulata-spontanea, P. yedoensis, P. fruticosa. P. kurilensis, P. subhirtella and P. incisa the presence of a second polyprenol family, i.e. the group of prenologues consisting of prenol -35, -36, -37, etc. up to -42 was detected.

Molecular analysis of cis-prenyl chain elongating enzymes

“…The chain length of Dol-P has been shown to be significantly species specific. For example, yeast contains dolichols of 14-17 isoprene units 18 whereas plant possesses dolichols with an isoprene unit of C 19-24 [19][20][21][22] and in mammalian cells the dolichols of 18-21 isoprene units are predominant. 23 The structural difference between the sugar carrier lipids and the bacterial counterpart is the longer prenyl chain and saturation of the α-isoprene unit.…”

Characterization of dehydrodolichyl diphosphate synthase of Arabidopsis thaliana, a key enzyme in dolichol biosynthesis

“…The resulting IPP serves as a substrate for condensation reactions with different allylic prenyl diphosphates leading to the synthesis of a series of linear prenyl diphosphates of increasing size which, in turn, serve as donors or intermediates in the synthesis of the wide range of plant isoprenoid end products [2]. Among these are long‐chain polyisoprenoid alcohols (polyprenols and dolichols), a family of compounds which have been reported to occur in a number of plants and shown to have a great heterogeneity in terms of carbon chain length [4–7].…”

The occurrence of geometric polyprenol isomers in the rubber‐producing plant, Eucommia ulmoides oliver

“…To develop high-resolution analysis of polyprenols, a variety of chromatographic conditions were tested. Previously, Swiezewska et al (8) reported a conventional polyprenol separation method using reversed-phase high-performance liquid chromatography (HPLC). Their findings showed that Prunus kurilensis has a broad distribution pattern of polyprenols with two distribution peaks centering at 19 and 34 mers.…”