The DNA Damage Response in Filamentous Fungi

Goldman, Gustavo H.; McGuire, Sarah Lea; Harris, Steven D.

doi:10.1006/fgbi.2002.1344

Cited by 72 publications

(54 citation statements)

References 82 publications

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“…These mechanisms include base excision repair (BER), nucleotide excision repair (NER), mutagenic repair and post-replication repair (Goldman et al 2002, Hjertvik et al 1998, Fishel and Kolodner 1995. In A. nidulans the UV-sensitive mutants (uvs) have been classified in different epistatic groups such as: UvsB, UvsC, UvsF and UvsI.…”

Section: Resultsmentioning

confidence: 99%

Cremophor EL stimulates mitotic recombination in uvsH//uvsH diploid strain of Aspergillus nidulans

Busso

Castro-Prado

2004

An. Acad. Bras. Ciênc.

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Cremophor EL is a solubilizer and emulsifier agent used in the pharmaceutical and foodstuff industries. The solvent is the principal constituent of paclitaxel's clinical formulation vehicle. Since mitotic recombination plays a crucial role in multistep carcinogenesis, the study of the recombinagenic potential of chemical compounds is of the utmost importance. In our research genotoxicity of cremophor EL has been studied by using an uvsH//uvsH diploid strain of Aspergillus nidulans. Since it spends a great part of its cell cycle in the G2 period, this fungus is a special screening system for the study of mitotic recombination induced by chemical substances. Homozygotization Indexes (HI) for paba and bi markers from heterozygous B211//A837 diploid strain were determined for the evaluation of the recombinagenic effect of cremophor EL. It has been shown that cremophor EL induces increase in mitotic crossing-over events at nontoxic concentrations (0.05 and 0.075% v/v).

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Section: Resultsmentioning

confidence: 99%

Cremophor EL stimulates mitotic recombination in uvsH//uvsH diploid strain of Aspergillus nidulans

Busso

Castro-Prado

2004

An. Acad. Bras. Ciênc.

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“…Isolation and characterization of strains sensitive to mutagens has led to an understanding of the range of DNA repair mechanisms. These include excision repair, recombination repair, photoreactivation repair, postreplication repair, and mismatch repair, all of which exist in Neurospora (287,364,692). Another mechanism, known as checkpoint control, affects the efficiency of DNA repair.…”

Section: Dna Repairmentioning

confidence: 99%

Lessons from the Genome Sequence ofNeurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism

Borkovich

Alex

Yarden

et al. 2004

Microbiol Mol Biol Rev

598

618

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We present an analysis of over 1,100 of the ∼10,000 predicted proteins encoded by the genome sequence of the filamentous fungus Neurospora crassa. Seven major areas of Neurospora genomics and biology are covered. First, the basic features of the genome, including the automated assembly, gene calls, and global gene analyses are summarized. The second section covers components of the centromere and kinetochore complexes, chromatin assembly and modification, and transcription and translation initiation factors. The third area discusses genome defense mechanisms, including repeat induced point mutation, quelling and meiotic silencing, and DNA repair and recombination. In the fourth section, topics relevant to metabolism and transport include extracellular digestion; membrane transporters; aspects of carbon, sulfur, nitrogen, and lipid metabolism; the mitochondrion and energy metabolism; the proteasome; and protein glycosylation, secretion, and endocytosis. Environmental sensing is the focus of the fifth section with a treatment of two-component systems; GTP-binding proteins; mitogen-activated protein, p21-activated, and germinal center kinases; calcium signaling; protein phosphatases; photobiology; circadian rhythms; and heat shock and stress responses. The sixth area of analysis is growth and development; it encompasses cell wall synthesis, proteins important for hyphal polarity, cytoskeletal components, the cyclin/cyclin-dependent kinase machinery, macroconidiation, meiosis, and the sexual cycle. The seventh section covers topics relevant to animal and plant pathogenesis and human disease. The results demonstrate that a large proportion of Neurospora genes do not have homologues in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. The group of unshared genes includes potential new targets for antifungals as well as loci implicated in human and plant physiology and disease

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“…nidulans has long been utilized as a model genetic system and the cell cycle and DNA damage response in this organism is well characterized (for review see Osmani and Ye 1996;Goldman et al 2002;Goldman and Kafer 2004;Osmani and Mirabito 2004). Temperature-sensitive mutants of the A. nidulans nimA kinase reversibly arrest in G 2 at the nonpermissive temperature of 42°even though the Cdc2/cyclinB kinase is fully activated (Osmani et al 1987).…”

Section: Mre11mentioning

confidence: 99%

A Point Mutation in theAspergillus nidulans sonBNup98 Nuclear Pore Complex Gene Causes Conditional DNA Damage Sensitivity

Souza

Hashmi

Horn³

et al. 2006

Genetics

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The nuclear pore complex (NPC) is embedded in the nuclear envelope where it mediates transport between the cytoplasm and nucleus and helps to organize nuclear architecture. We previously isolated sonB1, a mutation encoding a single amino acid substitution within the Aspergillus nidulans SONBn Nup98 NPC protein (nucleoporin). Here we demonstrate that this mutation causes marked DNA damage sensitivity at 42°. Although SONBn Nup98 has roles in the G 2 transition, we demonstrate that the G 2 DNA damage checkpoint is functional in the sonB1 mutant at 42°. The MRN complex is composed of MRE11, RAD50, and NBS1 and functions in checkpoint signaling, DNA repair, and telomere maintenance. At 42°w e find that the DNA damage response defect of sonB1 mutants causes synthetic lethality when combined with mutations in scaA NBS1 , the A. nidulans homolog of NBS1. We provide evidence that this synthetic lethality is independent of MRN cell cycle checkpoint functions or MREA MRE11-mediated DNA repair functions. We also demonstrate that the single A. nidulans histone H2A gene contains the C-terminal SQE motif of histone H2AX isoforms and that this motif is required for the DNA damage response. We propose that the sonB1 nucleoporin mutation causes a defect in a novel part of the DNA damage response.

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The DNA Damage Response in Filamentous Fungi

Cited by 72 publications

References 82 publications

Cremophor EL stimulates mitotic recombination in uvsH//uvsH diploid strain of Aspergillus nidulans

Cremophor EL stimulates mitotic recombination in uvsH//uvsH diploid strain of Aspergillus nidulans

Lessons from the Genome Sequence ofNeurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism

A Point Mutation in theAspergillus nidulans sonBNup98 Nuclear Pore Complex Gene Causes Conditional DNA Damage Sensitivity

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