The DraC usher in Dr fimbriae biogenesis of uropathogenic E. coli Dr+ strains

Zalewska-Piątek, Beata; Kur, Marta; Wilkanowicz, Sabina; Piątek, Rafał; Kur, Józef

doi:10.1007/s00203-010-0564-x

Cited by 4 publications

(3 citation statements)

References 37 publications

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“…The role of the FGL chaperone/usher biogenesis pathway of Dr fimbriae has been investigated in detail (174)(175)(176)(177)(178). To allow proper folding of the DraE adhesin subunits to occur, the DraC usher creates an assembly and secretion platform, and premature DraE subunit-subunit association is prevented by the DraB chaperone.…”

Section: Afa/dr Adhesinsmentioning

confidence: 99%

Pathogenesis of Human Diffusely Adhering Escherichia coli Expressing Afa/Dr Adhesins (Afa/Dr DAEC): Current Insights and Future Challenges

Servin

2014

Clin Microbiol Rev

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SUMMARY The pathogenicity and clinical pertinence of diffusely adhering Escherichia coli expressing the Afa/Dr adhesins (Afa/Dr DAEC) in urinary tract infections (UTIs) and pregnancy complications are well established. In contrast, the implication of intestinal Afa/Dr DAEC in diarrhea is still under debate. These strains are age dependently involved in diarrhea in children, are apparently not involved in diarrhea in adults, and can also be asymptomatic intestinal microbiota strains in children and adult. This comprehensive review analyzes the epidemiology and diagnosis and highlights recent progress which has improved the understanding of Afa/Dr DAEC pathogenesis. Here, I summarize the roles of Afa/Dr DAEC virulence factors, including Afa/Dr adhesins, flagella, Sat toxin, and pks island products, in the development of specific mechanisms of pathogenicity. In intestinal epithelial polarized cells, the Afa/Dr adhesins trigger cell membrane receptor clustering and activation of the linked cell signaling pathways, promote structural and functional cell lesions and injuries in intestinal barrier, induce proinflammatory responses, create angiogenesis, instigate epithelial-mesenchymal transition-like events, and lead to pks -dependent DNA damage. UTI-associated Afa/Dr DAEC strains, following adhesin-membrane receptor cell interactions and activation of associated lipid raft-dependent cell signaling pathways, internalize in a microtubule-dependent manner within urinary tract epithelial cells, develop a particular intracellular lifestyle, and trigger a toxin-dependent cell detachment. In response to Afa/Dr DAEC infection, the host epithelial cells generate antibacterial defense responses. Finally, I discuss a hypothetical role of intestinal Afa/Dr DAEC strains that can act as “silent pathogens” with the capacity to emerge as “pathobionts” for the development of inflammatory bowel disease and intestinal carcinogenesis.

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Section: Afa/dr Adhesinsmentioning

confidence: 99%

Pathogenesis of Human Diffusely Adhering Escherichia coli Expressing Afa/Dr Adhesins (Afa/Dr DAEC): Current Insights and Future Challenges

Servin

2014

Clin Microbiol Rev

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“…The development of urinary tract infection is usually by the ascending route ( Kaper et al., 2004 ; Yu et al., 2020 ). The main symptoms are difficulty in urination, dysuria, hematuria, pyuria, frequent urination, nycturia, pain in the lumbar region, suprapubic region, bladder tenderness, fever, and foul smelling urine ( Zalewska-Piątek et al., 2010 ; Schreiber et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Hospital Urinary Tract Infections in Healthcare Units on the Example of Mazovian Specialist Hospital Ltd

Trześniewska-Ofiara

Mendrycka

Cudo

et al. 2022

Front. Cell. Infect. Microbiol.

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Microbiological diagnostics is of great importance in limiting the spread of nosocomial infections. The information on etiological agents of infections and their susceptibility to antibiotics enables a quick response in the case of a suspected epidemic outbreak. The aim of this study is to analyze the incidence of nosocomial urinary tract infections among patients hospitalized in hospital wards over a period of 2 years and to determine the predominant etiological agent depending on the method of clinical specimen collection. Data from the Mazovian Specialist Hospital (MSH) in Radom constitute the material for the preparation of this study. Urine was collected using two methods. The first one was the method of collecting urine from the central stream, while the second method was urine collected from patients with a urinary catheter in place. The statistical calculations were conducted using the statistical software. Based on hospital data, it was shown that 5,870 urine tests were performed during the period under review, of which 2,070 were positive. The number of positive results in 2021 decreased by 2.84% compared to that in 2020. On the basis of the statistical analysis, differences in the occurrence of multiple strains were observed between catheter-based and midstream urine collection. Differences were observed especially for Acinetobacter baumannii, Candida albicans, Escherichia coli, and Pseudomonas aeruginosa. A. baumannii, C. albicans, and P. aeruginosa were significantly more frequently found in urine samples collected through the catheter than from the midstream. Furthermore, E. coli (51.56%) and Enterococcus species (25.46%) were more frequent when collected from the middle stream than when urine was collected through a catheter. However, for the strain K. pneumoniae, the results were comparable when urine was collected from catheterized patients (13.83%) and from midstream (13.35%). Urinary tract infection among hospitalized patients of the Mazovian Specialist Hospital in Radom was diagnosed quite frequently. In 2021, 32 more urine cultures were performed than in 2020. In the analyzed period, among all ordered urine cultures, 35.27% of samples were positive.

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“…The ability of UPEC to form biofilms is a major contributor to UTI severity, persistence, and recurrence. [10][11][12] UPEC biofilms render treatment with current antimicrobials difficult as they are often extremely tolerant to conventional antibiotics. 13 Treatment of uncomplicated acute UTIs normally involves administering a short course of antibiotics, and one of the most potent and commonly administered antibiotic therapies for UTIs is ciprofloxacin.…”

Section: Introductionmentioning

confidence: 99%

Eradicating uropathogenic Escherichia coli biofilms with a ciprofloxacin–dinitroxide conjugate

et al. 2019

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The DraC usher in Dr fimbriae biogenesis of uropathogenic E. coli Dr+ strains

Cited by 4 publications

References 37 publications

Pathogenesis of Human Diffusely Adhering Escherichia coli Expressing Afa/Dr Adhesins (Afa/Dr DAEC): Current Insights and Future Challenges

Pathogenesis of Human Diffusely Adhering Escherichia coli Expressing Afa/Dr Adhesins (Afa/Dr DAEC): Current Insights and Future Challenges

Hospital Urinary Tract Infections in Healthcare Units on the Example of Mazovian Specialist Hospital Ltd

Eradicating uropathogenic Escherichia coli biofilms with a ciprofloxacin–dinitroxide conjugate

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