The dynamic changes of main cell types in the microenvironment of sciatic nerves following sciatic nerve injury and the influence of let-7 on their distribution

Qian, Tianmei; Wang, Pan; Chen, Qianqian; Yi, Sheng; Liu, Qianyan; Wang, Hongkui; Wang, Shiyuan; Geng, Wenqing; Liu, Zihao; Li, Shiying

doi:10.1039/c8ra08298g

Cited by 19 publications

(9 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In turn, it is well known that bone marrow derived cells contribute to the endometrium and to endometriosis ( Du and Taylor, 2007 ). Schwann cells, fibroblasts and macrophages also play important roles in regulating the regeneration microenvironment and improving the regeneration effect through a variety of manual interventions ( Qian et al, 2019 ). Therefore, in peripheral nerves, stem/progenitor cells present potentials in the treatment, cure, and repair of damaged tissues secondary to injury and inflammation ( Mazzeo and Santos, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Laparoscopic morphological aspects and tentative explanation of the aetiopathogenesis of isolated endometriosis of the sciatic nerve: a review based on 267 patients

Possover¹

2021

Facts Views Vis ObGyn

View full text Add to dashboard Cite

Background: Endometriosis of the sciatic nerve (ESN) is considered a rare disease. How can endometriosis develop within the sciatic nerve; a structure which has nothing in common with the uterus either anatomically or functionally, and why it occurs in the absence of any retroperitoneal/parametric endometriosis, is unknown. A better understanding of the pathophysiology of this enigmatic disease may improve its diagnosis and therapy. Materials and Methods: From a pool of 452 patients operated for ESN, only patients with “isolated” endometriosis of the sciatic nerve” confirmed at laparoscopy were included in this study. Patients with suspicion of ESN by extension from a parametric, ovarian or other intraperitoneal deeply infiltrating endometriosis were excluded from this study. Main outcome measure: All information acquired during the preoperative patient’s medical history and clinical examination were collected and compared with the morphological aspects of the disease observed by the laparoscopic treatment. Patients were classified into three groups according to the time interval between the onset of sciatic pain and the time of surgery: less than 1 year (Group 1), between 1 and 3 years (Group 2), and more than 3 years (Group 3). Results: Two hundred sixty-seven consecutive patients were included in this study. In Group 1 (n=67), 76% of the patients presented with cyclical sciatica, without sensory or motor disorders of the lower limbs. Laparoscopic exploration found in the great majority of these patients only the presence of an isolated endometrioma in the nerve itself, the size of which was proportional to the time elapsed since the onset of pain. In Group 2 (n=83), pain had become constant in 91% of the patients with neurological disorders of the lower limb (foot drop, Trendelenburg gait, atrophied muscles) in about 30% of patients. Laparoscopic examination revealed, in addition to intraneural cystic lesions, a retroperitoneal fibrosis in more than 80% of the patients. In the third group (N=117), more than 80% of the patients presented with neurological disorders of the lower limb, with, on laparoscopic examination, massive retroperitoneal fibrosis with endometriomas in the nerve and adjacent pelvic wall muscles in all patients and an infiltration of the obturator nerve in 41% of patients. Conclusions: The different morphologic aspects of ESN do not correspond to different forms of the disease, but obviously to one single disease at different stages of its evolution. ENS starts first with the development of an endometrioma within the sciatic nerve, then develops in a second step a perineural fibrosis that expands into the whole retroperitoneal space and finally involves surrounding anatomical structures. The ESN is a very particular pathology because it induces a completely new aspect on the pathogenesis of endometriosis: all hypothesis of implanted endometrial cells following retrograde menstruation, angiogenic spread, lymphogenic spread or the metaplasia theory cannot explain the pathogenesis of this disease. ESN obviously does not develop from “genital metastatic cells”. A possible hypothesis for explanation the pathogenesis of ESN, could consist in the development of endometriosis of the nerve from progenitor stem cells present within the nerve, pluripotent cells which, for an as yet unknown reason (possibly in connection with iterative inflammations and micro-damages of the nerve itself), mutate and proliferate to form endometriosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Laparoscopic morphological aspects and tentative explanation of the aetiopathogenesis of isolated endometriosis of the sciatic nerve: a review based on 267 patients

Possover¹

2021

Facts Views Vis ObGyn

View full text Add to dashboard Cite

show abstract

“…A limitation of our current study was that macrophages were not included in these cell populations because the diameters of macrophage were too large to be captured in our current study by 10X Genomics. It was reported that macrophages made up about 2.4%-9% of cell populations in the intact peripheral nerves (Mueller et al, 2001;Qian et al, 2018). The biological functions of macrophages in peripheral nerves should be taken into consideration.…”

Section: Distinct Schwann Cell Sub-populations In Sn and Drgmentioning

confidence: 99%

Cell populations in neonatal rat peripheral nerves identified by single‐cell transcriptomics

Chen

Wang

et al. 2020

Glia

Self Cite

View full text Add to dashboard Cite

Peripheral nerves connect central nerves with target tissues and organs and execute vital signal transduction functions. Although sub‐types of neurons have been defined, the heterogeneity of cell populations in peripheral nerves, especially Schwann cells, has not been well demonstrated. Here, we collected sciatic nerves (SN) and dorsal root ganglia (DRG) from neonatal (1‐day old) rats and classified cell populations by high‐coverage single‐cell sequencing. A total of 10 types of cells, including endothelial cells, erythrocytes, fibroblasts, monocytic cells, neurons, neutrophils, pericytes, satellite cells, Schwann cells, and vascular smooth muscle cells, were identified by transcriptome‐based cell typing. The comparisons of cells in neonatal rat SN and DRG revealed distinct atlas in different tissue localizations. Investigations of ligand‐receptor interactions showed that there existed direct cell–cell communications between endothelial cells and fibroblasts in SN and among endothelial cells, fibroblasts, and vascular smooth muscle cells in DRG. Schwann cells in neonatal rats were further sub‐grouped to four sub‐types, including LOC100134871 and Hbb expressing Schwann cell sub‐type 1, Cldn19 and Emid1 expressing Schwann cell sub‐type 2, Timp3 and Col5a3 expressing Schwann cell sub‐type 3, and Cenpf and Mki67 expressing Schwann cell sub‐type 4. These Schwann cell sub‐types exhibited distinct genetic features and functional enrichments. Collectively, our results illustrated the diversity and cellular complexity of peripheral nerves at the neonatal stage and revealed the heterogeneity of Schwann cells in the peripheral nervous system.

show abstract

“…Following axonal reinnervation of the target, the dedifferentiated Schwann cells redifferentiate into myelinating Schwann cells to myelinate the newly formed axon and maintain homeostasis [59,60]. In addition to Schwann cells, all other cell types of the peripheral nerves (macrophages, fibroblasts, endothelial cells, pericytes) respond to injury with proliferation and contribute to an efficient regeneration [60,61]. In the following, we discuss how exosomes contribute to an efficient regeneration between these different cell types of the peripheral nerves.…”

Section: The Role Of Exosomes During Peripheral Nerve Regenerationmentioning

confidence: 99%

The role of exosomes in intercellular and inter‐organ communication of the peripheral nervous system

2022

View full text Add to dashboard Cite

Exosomes, nano‐sized extracellular vesicles, are produced via the endosomal pathway and released in the extracellular space upon fusion of multivesicular bodies with the plasma membrane. Recent evidence shows that these extracellular vesicles play a key role in cell‐to‐cell communication. Exosomes transport bioactive proteins, mRNAs, and microRNA (miRNAs) in an active form to adjacent cells or to distant organs. In this review, we focus on the role of exosomes in peripheral nerve maintenance and repair, as well as peripheral nerve/organ crosstalk, and discuss the potential benefits of exploiting exosomes for treating PNS injuries. In addition, we will highlight the emerging role of exosomes as new important vehicles for physiological systemic crosstalk failures, which could lead to organ dysfunction during neuroinflammation or aging.

show abstract

The dynamic changes of main cell types in the microenvironment of sciatic nerves following sciatic nerve injury and the influence of let-7 on their distribution

Cited by 19 publications

References 47 publications

Laparoscopic morphological aspects and tentative explanation of the aetiopathogenesis of isolated endometriosis of the sciatic nerve: a review based on 267 patients

Laparoscopic morphological aspects and tentative explanation of the aetiopathogenesis of isolated endometriosis of the sciatic nerve: a review based on 267 patients

Cell populations in neonatal rat peripheral nerves identified by single‐cell transcriptomics

The role of exosomes in intercellular and inter‐organ communication of the peripheral nervous system

Contact Info

Product

Resources

About