The Dynamic Responses of Cell Walls in Resurrection Plants During Dehydration and Rehydration

Chen, Peilei; Jung, Niklas Udo; Giarola, Valentino; Bartels, Dorothea

doi:10.3389/fpls.2019.01698

Cited by 31 publications

(27 citation statements)

References 154 publications

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“…Additionally, a higher number of pectin esterases were induced in tolerant Selaginella species in comparison to the sensitive species (Additional file 6: Figure S6a). These results suggest that as previously reported cell wall modification plays an important role in desiccation tolerance plants including Selaginella species [41]. Minor carbohydrate metabolism showed a significant difference between tolerant species during DH, where S. sellowii showed 7 differentially induced transcripts classified as raffinose family genes ( Fig.…”

Section: Common and Specific Mechanisms Involved In The Acquisition Osupporting

confidence: 86%

“…However, tolerant species induced a higher proportion of pectin esterases during DH in comparison to the sensitive one. The activity of pectin esterases could modify cell wall structure [41] and qRT-PCR analysis corroborated the upregulation of a pectin methylesterase inhibitor in both tolerant species indicating that this mechanism is also present in S. sellowii. Although a lower number of enriched genes was observed in the cell wall modification category in S. sellowii, similarities with S. lepidophylla such as induction of precursors and hemicellulose synthesis were observed.…”

Section: Discussionmentioning

confidence: 65%

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Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species

et al. 2020

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Background Desiccation tolerant Selaginella species evolved to survive extreme environmental conditions. Studies to determine the mechanisms involved in the acquisition of desiccation tolerance (DT) have focused on only a few Selaginella species. Due to the large diversity in morphology and the wide range of responses to desiccation within the genus, the understanding of the molecular basis of DT in Selaginella species is still limited. Results Here we present a reference transcriptome for the desiccation tolerant species S. sellowii and the desiccation sensitive species S. denticulata. The analysis also included transcriptome data for the well-studied S. lepidophylla (desiccation tolerant), in order to identify DT mechanisms that are independent of morphological adaptations. We used a comparative approach to discriminate between DT responses and the common water loss response in Selaginella species. Predicted proteomes show strong homology, but most of the desiccation responsive genes differ between species. Despite such differences, functional analysis revealed that tolerant species with different morphologies employ similar mechanisms to survive desiccation. Significant functions involved in DT and shared by both tolerant species included induction of antioxidant systems, amino acid and secondary metabolism, whereas species-specific responses included cell wall modification and carbohydrate metabolism. Conclusions Reference transcriptomes generated in this work represent a valuable resource to study Selaginella biology and plant evolution in relation to DT. Our results provide evidence of convergent evolution of S. sellowii and S. lepidophylla due to the different gene sets that underwent selection to acquire DT

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Section: Common and Specific Mechanisms Involved In The Acquisition Osupporting

confidence: 86%

Section: Discussionmentioning

confidence: 65%

Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species

et al. 2020

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“…Our knowledge is very limited, but it suggests that interesting research opportunities exist and will feed scientific discussion in the future [127,128]. While research into food and energy crop plants has the obvious attractions of potentially leading to results with translational potential, investigating plants surviving in extreme environments may yield results with more pronounced impact in the long term [129].…”

Section: Perspective: Open Questions and Future Challengesmentioning

confidence: 99%

The Role of Mechanoperception in Plant Cell Wall Integrity Maintenance

Bacete

Hamann

2020

Plants

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The plant cell walls surrounding all plant cells are highly dynamic structures, which change their composition and organization in response to chemical and physical stimuli originating both in the environment and in plants themselves. They are intricately involved in all interactions between plants and their environment while also providing adaptive structural support during plant growth and development. A key mechanism contributing to these adaptive changes is the cell wall integrity (CWI) maintenance mechanism. It monitors and maintains the functional integrity of cell walls by initiating adaptive changes in cellular and cell wall metabolism. Despite its importance, both our understanding of its mode of action and knowledge regarding the molecular components that form it are limited. Intriguingly, the available evidence implicates mechanosensing in the mechanism. Here, we provide an overview of the knowledge available regarding the molecular mechanisms involved in and discuss how mechanoperception and signal transduction may contribute to plant CWI maintenance.

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“…CpCRP1, CpGRP1, CpWAK1, and pcC13-62 genes encode cell-wall-localized proteins in the resurrection plant C. plantagineum (Chen et al 2019;Giarola et al 2015b). The abundance of the proteins is modulated during the dehydration and rehydration cycle and thus these proteins are candidates to participate in cell wall folding mechanisms (Giarola et al 2015b(Giarola et al , 2016(Giarola et al , 2018Jung et al 2019).…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

The dehydration- and ABA-inducible germin-like protein CpGLP1 from Craterostigma plantagineum has SOD activity and may contribute to cell wall integrity during desiccation

Giarola

Chen

Dulitz

et al. 2020

Planta

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Main conclusion CpGLP1 belongs to the large group of germin-like proteins and comprises a cell wall-localized protein which has superoxide dismutase activity and may contribute towards ROS metabolism and cell wall folding during desiccation. Abstract The plant cell wall is a dynamic matrix and its plasticity is essential for cell growth and processing of environmental signals to cope with stresses. A few so-called resurrection plants like Craterostigma plantagineum survive desiccation by implementing protection mechanisms. In C. plantagineum, the cell wall shrinks and folds upon desiccation to avoid mechanical and oxidative damage which contributes to cell integrity. Despite the high toxic potential, ROS are important molecules for cell wall remodeling processes as they participate in enzymatic reactions and act as signaling molecules. Here we analyzed the C. plantagineum germin-like protein 1 (CpGLP1) to understand its contribution to cell wall folding and desiccation tolerance. The analysis of the CpGLP1 sequence showed that this protein does not fit into the current GLP classification and forms a new group within the Linderniaceae. CpGLP1 transcripts accumulate in leaves in response to dehydration and ABA, and mannitol treatments transiently induce CpGLP1 transcript accumulation supporting the participation of CpGLP1 in desiccation-related processes. CpGLP1 protein from cell wall protein extracts followed transcript accumulation and protein preparations from bacteria overexpressing CpGLP1 showed SOD activity. In agreement with cell wall localization, CpGLP1 interacts with pectins which have not been reported for GLP proteins. Our data support a role for CpGLP1 in the ROS metabolism related to the control of cell wall plasticity during desiccation in C. plantagineum.

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The Dynamic Responses of Cell Walls in Resurrection Plants During Dehydration and Rehydration

Cited by 31 publications

References 154 publications

Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species

Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species

The Role of Mechanoperception in Plant Cell Wall Integrity Maintenance

The dehydration- and ABA-inducible germin-like protein CpGLP1 from Craterostigma plantagineum has SOD activity and may contribute to cell wall integrity during desiccation

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