The e Subunit Gene of Murine F1F0-ATP Synthase

Swartz, Deborah A.; Park, Eric I.; Visek, Willard J.; Kaput, Jim

doi:10.1074/jbc.271.34.20942

Cited by 25 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genes involved in the glucose-induced insulin secretion cascade of pancreatic beta-cells are located in the region, such as ATP5I (ATP synthase, H + transporting, mitochondrial F 0 complex, subunit E) [42, 43], CPLX1 (complexin 1) [44, 45], GAK (cyclin G associated kinase) in association with CDK5 (cyclin-dependent protein kinases 5) [46–50], and CRIPAK (cysteine-rich PAK1 inhibitor), which is a negative regulator of PAK1 (p21 protein (Cdc42/Rac-) activated kinase 1) [51, 52]. …”

Section: Discussionmentioning

confidence: 99%

Frequent Loss of Genome Gap Region in 4p16.3 Subtelomere in Early-Onset Type 2 Diabetes Mellitus

Kudo

Emi

Ishigaki

et al. 2011

Experimental Diabetes Research

View full text Add to dashboard Cite

A small portion of Type 2 diabetes mellitus (T2DM) is familial, but the majority occurs as sporadic disease. Although causative genes are found in some rare forms, the genetic basis for sporadic T2DM is largely unknown. We searched for a copy number abnormality in 100 early-onset Japanese T2DM patients (onset age <35 years) by whole-genome screening with a copy number variation BeadChip. Within the 1.3-Mb subtelomeric region on chromosome 4p16.3, we found copy number losses in early-onset T2DM (13 of 100 T2DM versus one of 100 controls). This region surrounds a genome gap, which is rich in multiple low copy repeats. Subsequent region-targeted high-density custom-made oligonucleotide microarray experiments verified the copy number losses and delineated structural changes in the 1.3-Mb region. The results suggested that copy number losses of the genes in the deleted region around the genome gap in 4p16.3 may play significant roles in the etiology of T2DM.

show abstract

Section: Discussionmentioning

confidence: 99%

Frequent Loss of Genome Gap Region in 4p16.3 Subtelomere in Early-Onset Type 2 Diabetes Mellitus

Kudo

Emi

Ishigaki

et al. 2011

Experimental Diabetes Research

View full text Add to dashboard Cite

show abstract

“…Additionally, we found ATP5ME and NDUFAF2 were differentially expressed. ATP5ME encodes the e subunit of Fo complex that constitutes the membrane-spanning component of adenosine-triphosphate synthase (42), and the encoding product of NDUFAF2 is the one of the components of complex I in the electron transport chain (43). These results are accordant to impaired complex I, II, and IV in the oxidative phosphorylation system (44,45) and reduced activity of complex in the mitochondrial electron transport chain (46,47) observed by other researchers in patients with schizophrenia.…”

Section: Discussionmentioning

confidence: 76%

Transcriptomics Analysis Reveals Shared Pathways in Peripheral Blood Mononuclear Cells and Brain Tissues of Patients With Schizophrenia

Song

Liu

et al. 2021

Front. Psychiatry

View full text Add to dashboard Cite

Background: Schizophrenia is a serious mental disorder with complicated biological mechanisms. Few studies explore the transcriptional features that are shared in brain tissue and peripheral blood. In the present study, we aimed to explore the biological pathways with similar expression patterns in both peripheral blood mononuclear cells (PBMCs) and brain tissues.Methods: The present study used transcriptomics technology to detect mRNA expression of PBMCs of 10 drug-naïve patients with schizophrenia and 20 healthy controls. Transcriptome data sets of brain tissue of patients with schizophrenia downloaded from public databases were also analyzed in our study. The biological pathways with similar expression patterns in the PBMCs and brain tissues were uncovered by differential expression analysis, weighted gene co-expression network analysis (WGCNA), and pathway analysis. Finally, the expression levels of differential expressed genes (DEGs) were validated by real-time fluorescence quantitative polymerase chain reaction (qPCR) in another 12 drug-naïve patients with schizophrenia and 12 healthy controls.Results: We identified 542 DEGs, 51 DEGs, 732 DEGs, and 104 DEGs in PBMCs, dorsolateral prefrontal cortex, anterior cingulate gyrus, and nucleus accumbent, respectively. Five DEG clusters were recognized as having similar gene expression patterns in PBMCs and brain tissues by WGCNA. The pathway analysis illustrates that these DEG clusters are mainly enriched in several biological pathways that are related to phospholipid metabolism, ribosome signal transduction, and mitochondrial oxidative phosphorylation. The differential significance of PLAAT3, PLAAT4, PLD2, RPS29, RPL30, COX7C, COX7A2, NDUFAF2, and ATP5ME were confirmed by qPCR.Conclusions: This study finds that the pathways associated with phospholipid metabolism, ribosome signal transduction, and energy metabolism have similar expression patterns in PBMCs and brain tissues of patients with schizophrenia. Our results supply a novel insight for revealing the pathogenesis of schizophrenia and might offer a new approach to explore potential biological markers of peripheral blood in schizophrenia.

show abstract

“…It is important to emphasize that Western blots demonstrated that RBF-specific antibodies do not cross-react to mitochondrial antigens (unpublished observation). The mitochondrial ATP synthase chain is a distinct polypeptide that is a nonenzymatic component of the multiprotein complex located in the mitochondrial membrane that synthesizes ATP (66). This identity supports that RBF protein would form protein-protein interactions, even though the two proteins have very distinct morphological functions.…”

Section: Discussionmentioning

confidence: 81%

Interactions of the Nuclear Matrix-Associated Steroid Receptor Binding Factor with Its DNA Binding Element in the c-myc Gene Promoter

et al. 2000

View full text Add to dashboard Cite

Steroid receptor binding factor (RBF) was originally isolated from avian oviduct nuclear matrix. When bound to avian genomic DNA, RBF generates saturable high-affinity binding sites for the avian progesterone receptor (PR). Recent studies have shown that RBF binds to a 54 bp element in the 5'-flanking region of the progesterone-regulated avian c-myc gene, and nuclear matrix-like attachment sites flank the RBF element [Lauber et al. (1997) J. Biol. Chem. 272, 24657-24665]. In this paper, electrophoretic mobility shift assays (EMSAs) and S1 nuclease treatment are used to demonstrate that the RBF-maltose binding protei (MBP) fusion protein binds to single-stranded DNA of its element. Only the N-terminal domain of RBF binds the RBF DNA element as demonstrated by southwestern blot analyses, and by competition EMSAs between RBF-MBP and the N-terminal domain. Mass spectrometric analysis of the C-terminal domain of RBF demonstrates its potential to form noncovalent protein-protein interactions via a potential leucine-isoleucine zipperlike structure, suggesting a homo- and/or possible heterodimer structure in solution. These data support that the nuclear matrix binding site (acceptor site) for PR in the c-myc gene promoter is composed of RBF dimers bound to a specific single-stranded DNA element. The dimers of RBF are generated by C-terminal leucine zipper and the DNA binding occurs at the N-terminal parallel beta-sheet DNA binding motif. This complex is flanked by nuclear matrix attachment sites.

show abstract

The e Subunit Gene of Murine F1F0-ATP Synthase

Cited by 25 publications

References 24 publications

Frequent Loss of Genome Gap Region in 4p16.3 Subtelomere in Early-Onset Type 2 Diabetes Mellitus

Frequent Loss of Genome Gap Region in 4p16.3 Subtelomere in Early-Onset Type 2 Diabetes Mellitus

Transcriptomics Analysis Reveals Shared Pathways in Peripheral Blood Mononuclear Cells and Brain Tissues of Patients With Schizophrenia

Interactions of the Nuclear Matrix-Associated Steroid Receptor Binding Factor with Its DNA Binding Element in the c-myc Gene Promoter

Contact Info

Product

Resources

About