The Effect Hypoglycemic of Ethanol Extract Combination Red Betel Leaf (Piper crocatum) and Dayak Onion (Eleutherine palmifolia Merr) in Streptozotocin-Induced

Abstract

Background: Red betel leaf and Dayak onion is used traditionally to treat diabetes mellitus, The use of this combination of extracts to assess the effectiveness of the combination therapy is getting better by working synergistically. Objective: This study aimed to determine the hypoglycemic effect and the effective dose of ethanol extract combination of red betel leaf and Dayak onion of male rats (Rattus norvegicus). Methods: The number of 35 male rats were divided into 7 groups and each group consisted of five rats. The first group (normal control) was given Na CMC; the second group (negative control) streptozotocin-induced ; the third group (positive control) glibenclamide , while the 4 th , 5 th ,6 th , and 7 th were successively given a single dose of ethanol extract of red betel leaf 25 mg/KgBB, a single dose of Dayak onion50 mg/ KgBB, combination red betel leaf and forest onion bulbs, 25+50 and 50+100 mg/kg bb. Each rat group was intraperitoneally (IP) induced with STZ 40 mg/KgBB and then treated for 21 days. The hypoglycemic effect was tested by using the God-Pap method. Result: Phytochemical analysis revealed that flavonoids, tannins, saponins, phytosterols and phenols were present in ethanol extract of red betel leaf and forest onion bulbs. The study concluded that the combination of red betel leaf and Dayak onion at doses 25 mg/KgBB and 50 mg/KgBB were synergistic in reducing glucose. Conclusion: In conclusion, the ethanol extract combination of red betel leaf and dayak onion have hypoglycemic effects.

Pharmacognostic Profile and Antidiabetic Activity of Eleutherine bulbosa Mills. Bulbs from East Kalimantan, Indonesia

“…The test results of several pharmacognostic parameters show that the E. bulbosa bulbs obtained in the area of Kota Bangun, Kutai Kertanegara, East Kalimantan, Indonesia, meet standardization values following established regulations 26,27 and previous studies 17,30 . Meanwhile, both extracts' antidiabetic activity assay results (obtained from different extraction methods) show consistency in potential activity as reported in previous studies 3,6,7,10,28,31,32 . Therefore, E. bulbosa bulbs can meet pharmacognostic criteria and have the antidiabetic activity to be used as a source of raw materials for antidiabetic herbal medicines.…”

“…Blood glucose was checked using a glucose strip test 3 days after STZ induction. After blood sugar rose from normal, the mice were given extract treatment for 14 days 7,10,28 . On the 15th day, blood sugar levels were measured again, and data analysis was carried out to see changes in blood glucose levels before and after giving the sample.…”

AKTIVITAS ANTIDIABETES EKSTRAK ETANOL DAUN SIRIH MERAH (Piper crocatum) PADA TIKUS PUTIH RETINOPATI DIABETIK

“…Kandungan kimia dalam ekstrak etanol daun sirih merah 96% yakni tanin, saponin, triterpenoid, serta flavonoid. Flavonoid pada daun sirih merah berupa antosianidin flavonon, auron, isoflavon, katekin, serta kalkon (Anggi and Magfirah, 2019).…”

Organoleptic Analysis, Quality Requirement, And Color Determination of Tilapia Nuggets with Eleutherine palmifolia Extract Coating

“…It shows that ethanol extract has a higher ability than aqueous extract to inhibit the activity of alpha glucosidase enzyme. Phytochemical analysis shows that Dayak onion ethanol 459 extract contains flavonoids, tannins, saponins, phytosterols and phenols (Anggi & Maghfirah, 2019). Dayak onion powder contains polyphenol and flavonoid compounds which are often referred as natural antioxidants, both of which work together to ward off free radicals (Hendrawan et al, 2020).…”