The Effect Infrared and Ultraviolet Radiation in the Development of the Cells in the Porous Permeable Titanium Nickel Based Alloy Scaffold

Gunther, S.; Dambayev, G. Ts.; Kokorev, O. V.

doi:10.18502/kms.v2i1.789

Cited by 2 publications

(5 citation statements)

References 3 publications

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“…41−44 Their physicochemical, mechanical, and technological properties are suitable for use as biocompatible matrices for cell suspensions and prolonged vital activity of the cells immobilized in matrices. 45 Experience using porous nickel−titanium incubators according to Gunter et al 46 revealed their inherent disadvantages. Manufacturing methods limit the total porosity to 70%, which accordingly limits the relative volume of the contained cells' suspension.…”

Section: Introductionmentioning

confidence: 99%

“…At the same time, nickel−titanium fibrous matrices have a porosity of more than 90%, a completely permeable structure, and depending on the density of the fibers, their mechanical properties can be controlled. 46 In this regard, the experimental study of the reparative mechanisms underlying the correction of disorders that occur in CCl 4 -induced hepatitis with tissueengineered structures from a fibrous-permeable matrix of nickel−titanium based on hepatocytes is relevant. This is a pilot study on the effectiveness of using a new fibrous matrix made from NiTi wire in maintaining the function of a liver damaged by a chemical agent.…”

Section: Introductionmentioning

confidence: 99%

“…Experience using porous nickel–titanium incubators according to Gunter et al revealed their inherent disadvantages. Manufacturing methods limit the total porosity to 70%, which accordingly limits the relative volume of the contained cells’ suspension.…”

Section: Introductionmentioning

confidence: 99%

“…The bulk array of porous nickel–titanium is not completely permeable, as there are up to 3% closed pores. At the same time, nickel–titanium fibrous matrices have a porosity of more than 90%, a completely permeable structure, and depending on the density of the fibers, their mechanical properties can be controlled . In this regard, the experimental study of the reparative mechanisms underlying the correction of disorders that occur in CCl 4 -induced hepatitis with tissue-engineered structures from a fibrous-permeable matrix of nickel–titanium based on hepatocytes is relevant.…”

Section: Introductionmentioning

confidence: 99%

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Engineered Fibrous NiTi Scaffolds with Cultured Hepatocytes for Liver Regeneration in Rats

Kokorev

Марченко

Хлусов

et al. 2023

ACS Biomater. Sci. Eng.

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At present, the use of alternative systems to replenish the lost functions of hepatic metabolism and partial replacement of liver organ failure is relevant, due to an increase in the incidence of various liver disorders, insufficiency, and cost of organs for transplantation, as well as the high cost of using the artificial liver systems. The development of low-cost intracorporeal systems for maintaining hepatic metabolism using tissue engineering, as a bridge before liver transplantation or completely replacing liver function, deserves special attention. In vivo applications of intracorporeal fibrous nickel–titanium scaffolds (FNTSs) with cultured hepatocytes are described. Hepatocytes cultured in FNTSs are superior to their injections in terms of liver function, survival time, and recovery in a CCl4-induced cirrhosis rats’ model. 232 animals were divided into 5 groups: control, CCl4-induced cirrhosis, CCl4-induced cirrhosis followed by implantation of cell-free FNTSs (sham surgery), CCl4-induced cirrhosis followed by infusion of hepatocytes (2 mL, 107 cells/mL), and CCl4-induced cirrhosis followed by FNTS implantation with hepatocytes. Restoration of hepatocyte function in the FNTS implantation with the hepatocytes group was accompanied by a significant decrease in the level of aspartate aminotransferase (AsAT) in blood serum compared to the cirrhosis group. A significant decrease in the level of AsAT was noted after 15 days in the infused hepatocytes group. However, on the 30th day, the AsAT level increased and was close to the cirrhosis group due to the short-term effect after the introduction of hepatocytes without a scaffold. The changes in alanine aminotransferase (AlAT), alkaline phosphatase (AlP), total and direct bilirubin, serum protein, triacylglycerol, lactate, albumin, and lipoproteins were similar to those in AsAT. The survival time of animals was significantly longer in the FNTS implantation with hepatocytes group. The obtained results showed the scaffolds’ ability to support hepatocellular metabolism. The development of hepatocytes in FNTS was studied in vivo using 12 animals using scanning electron microscopy. Hepatocytes demonstrated good adhesion to the scaffold wireframe and survival in allogeneic conditions. Mature tissue, including cellular and fibrous, filled the scaffold space by 98% in 28 days. The study shows the extent to which an implantable “auxiliary liver” compensates for the lack of liver function without replacement in rats.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Engineered Fibrous NiTi Scaffolds with Cultured Hepatocytes for Liver Regeneration in Rats

Kokorev

Марченко

Хлусов

et al. 2023

ACS Biomater. Sci. Eng.

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“…Разработка и детальное изучение физико-биологических основ применения сплавов из никелида титана (TiNi), как имплантационного материала, вот уже в течение последних 40 лет проводится группой исследователей под руководством заслуженного деятеля науки, профессора В.Э. Гюнтера [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. Так, в период 1975-1981 г.г.…”

Section: Introductionunclassified

Experimental Study of Integration Features of Porous Permeable and Mesh Titanium Nickelide in Vivo

Mirzoev¹,

Shakirov

Porokhova³

et al. 2020

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Objective: Morphological assessment of the integration features of the combined use of porous permeable and textile mesh titanium nickelide with bone structures of the recipient zone. Methods: The study was carried out on 20 Chinchilla rabbits of both sexes at the age of 1-1.5 years with a bodyweight of 2500-4000 g. In animals, the artificial defect was created in one of the areas of the lower jaw, where a porous permeable titanium nickelide was installed, wrapped in a textile mesh version of it was installed. Animals were removed from the experiment for 30, 90, 180, and 360 days of research via intraperitoneal injection of mortal dose 1% solution thiopental sodium. The material for histological research was fixed in 10% buffered formalin during 12 hours after then washed with water and decalcified. The prepared sections were stained with hematoxylin-eosin. Microscopic examination of the stained preparations and photography were carried out using a light microscope Axioscope 40 (Zeiss, Germany) via a digital camera (Canon, Japan). Results: Studies have shown that with the combined use of porous permeable and textile mesh titanium nickelide, the artificially created defects of the mandibula between both contacting surfaces are formed directly, which leads to stable fixing. The porous structure of the material, its hysteresis behavior with organism tissues, provides widespread regeneration of cells and formed a homogeneous mature bone tissue bone tissue, both in the pores and around the structure. Conclusions: These and other facts indicate the high integration properties of the material that is investigated, which ensures optimal growth and population of osteogenic cells in the vicinity of defective bone areas in a growing organism. Keywords: Bone defect, osteointegration, regeneration, titanium nickelide, porous permeable implant.

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The Effect Infrared and Ultraviolet Radiation in the Development of the Cells in the Porous Permeable Titanium Nickel Based Alloy Scaffold

Abstract: The respond of the cells to electromagnetic radiation depends on many factors,

Cited by 2 publications

References 3 publications

Engineered Fibrous NiTi Scaffolds with Cultured Hepatocytes for Liver Regeneration in Rats

Engineered Fibrous NiTi Scaffolds with Cultured Hepatocytes for Liver Regeneration in Rats

Experimental Study of Integration Features of Porous Permeable and Mesh Titanium Nickelide in Vivo

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