The Effect of 3D Construction Culture of Human Chondrocytes Using Alginate Sponge

Lee, Jin Sang; Kim, Byung Gon; Kim, Min Soo; Lee, Seung-Jae; Kim, Sung Won; Cho, Dong‐Woo; Kim, Sang Woo; Lim, Geun Bae

doi:10.4028/0-87849-415-4.883

Cited by 1 publication

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“…Fresh human cartilage was obtained from a nasal septum after operation. The cartilage fragments were dissected under sterile conditions and then were subjected to collagenase digestion at 37 °C for 12–18 h (Lee et al ., ). The resulting cell suspension was passed through a sterile 250 µm polypropylene mesh filter.…”

Section: Methodsmentioning

confidence: 97%

An additive manufacturing-based PCL-alginate-chondrocyte bioprinted scaffold for cartilage tissue engineering

Kundu

Shim

Jang

et al. 2013

J Tissue Eng Regen Med

481

315

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Regenerative medicine is targeted to improve, restore or replace damaged tissues or organs using a combination of cells, materials and growth factors. Both tissue engineering and developmental biology currently deal with the process of tissue self-assembly and extracellular matrix (ECM) deposition. In this investigation, additive manufacturing (AM) with a multihead deposition system (MHDS) was used to fabricate three-dimensional (3D) cell-printed scaffolds using layer-by-layer (LBL) deposition of polycaprolactone (PCL) and chondrocyte cell-encapsulated alginate hydrogel. Appropriate cell dispensing conditions and optimum alginate concentrations for maintaining cell viability were determined. In vitro cell-based biochemical assays were performed to determine glycosaminoglycans (GAGs), DNA and total collagen contents from different PCL-alginate gel constructs. PCL-alginate gels containing transforming growth factor-β (TGFβ) showed higher ECM formation. The 3D cell-printed scaffolds of PCL-alginate gel were implanted in the dorsal subcutaneous spaces of female nude mice. Histochemical [Alcian blue and haematoxylin and eosin (H&E) staining] and immunohistochemical (type II collagen) analyses of the retrieved implants after 4 weeks revealed enhanced cartilage tissue and type II collagen fibril formation in the PCL-alginate gel (+TGFβ) hybrid scaffold. In conclusion, we present an innovative cell-printed scaffold for cartilage regeneration fabricated by an advanced bioprinting technology.

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Section: Methodsmentioning

confidence: 97%