The Effect of a Combination of Eucommia ulmoides and Achyranthes japonica (KGC08EA) on Body Weight in High-Fat Diet-Induced Obese Mice

Abstract

In this study, a combination of Eucommia ulmoides and Achyranthes japonica (KGC08EA) demonstrated anti-obesity effects related to energy and lipid metabolism in vivo. To confirm their effectiveness in animals, high-fat diet (HFD) induced C57BL/6J mice were orally administrated KGC08EA for 9 weeks. The experimental groups were as follows: NC (C57BL/6J on a normal diet), C (HFD), PC (Coleus forskohlii, 100 mg/kg b.w.), EA100 (KGC08EA, 100 mg/kg b.w.), EA200 (KGC08EA, 200 mg/kg b.w.), EA400 (KGC08EA, 400 mg/kg b.w.), and EA800 (KGC08EA, 800 mg/kg b.w.). KGC08EA significantly decreased body weight, total fat and serum leptin, alanine aminotransferase, triglycerides, total cholesterol, and low density lipoprotein but significantly increased serum adiponectin. KGC08EA enhanced levels of energy and lipid metabolism modulators, such as phosphorylated adenosine monophosphate activated protein kinase, glucagon-like peptied 1 receptor, peroxisome proliferator-activated receptor gamma, acetyl-CoA carboxylase, and hormone-sensitive lipase, in liver and epididymal fat. These results suggest that KGC08EA has potential use as a health food for obesity.