The effect of a cortisol analogue and transport stress on the frequency of occurrence of micronuclei in erythrocytes of the peripheral blood of sterlet Acipenser ruthenus L.

Kamshilova, T. B.; Mikryakov, V. R.; Mikryakov, D. V.

doi:10.1134/s1995082913020041

Cited by 5 publications

(6 citation statements)

References 3 publications

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“…At the same time, it is well-known that the higher level of glucocorticoids provokes increasing the number of RBCs with chromosome aberrations. (17) This was discovered in the analysis of buccal epithelium in our study. The cytogram analysis in the experimental groups showed that there were fewer normal cells and more cells with signs of cytological and chromosomal abnormalities, in comparison with the control groups.…”

Section: Resultssupporting

confidence: 67%

“…The results of the RBC electrophoretic mobility study were identical to the findings that we received earlier when studying different kinds of stresses. (17) A literature review shows that the RBC electrophoretic mobility index decreases if there are some pathologies, such as chronic kidney disease, chronic brain ischemia, respiratory diseases, ischemic heart disease, or oncological diseases, as well as all kinds of intoxications. It was noted earlier that a decrease in RBC electrophoretic mobility Table 1.…”

Section: Resultsmentioning

confidence: 99%

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Electrophoretic Mobility of Red Blood Cells and Micronucleus Test in Exfoliated Buccal Cells as Stress Intensity Markers

Deryugina¹,

Ivashchenko²,

Ignatyev³

et al. 2018

IJBM

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The purpose of this study was to investigate the electrophoretic mobility of red blood cells (RBCs), cytomorphological and cytogenetical indices in exfoliated buccal epithelial cells of rats during the development of stress reaction. Experiments were carried out on 60 white non-pedigree pubescent rats weighing 180-220 g. For stress modeling, the animals of experimental groups received a single intraperitoneal injection of epinephrine hydrochloride solution (0.1 mg/kg) or injections for 3 days of the experiment. Both the single and the repeated adrenalin injections provoked a decrease in the level of the RBC electrophoretic mobility. With a single adrenalin injection, the changes in RBC electrophoretic mobility are not so abrupt, with the subsequent development of the adaptive response and reparation of cytogenetic damage. With repeated adrenalin injections, the changes in RBC electrophoretic mobility are abrupt, and the number of pathological epithelial cells increases.

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Section: Resultssupporting

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Electrophoretic Mobility of Red Blood Cells and Micronucleus Test in Exfoliated Buccal Cells as Stress Intensity Markers

Deryugina¹,

Ivashchenko²,

Ignatyev³

et al. 2018

IJBM

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“…Cortisol acts at the genome, inducing somatic mutations in the hemato poietic cells, including erythrocytes, changing their hereditary properties and leading to their malfunctions (Wendelaar, 1997;Kelestemur & Ozdemir 2010, Kamshilova et al, 2013.…”

Section: Groupsmentioning

confidence: 99%

DNA Damage in Hybrid Tilapia (Oreochromis niloticus x O. aureus) Exposed to Short-Transport Process

Turan

Ergenler

2021

Natural and Engineering Sciences

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In this study, DNA damage in hybrid tilapia exposed to the short-transport process has been investigated. Gill samples were taken from tilapia which after immediately from the transport process (t0 group), after 6 hours from transport process (t6 group), after 12 hours from transport process (t12 group), after 24 hours from transport process (t24 group) and not applied transport process (control group) have been investigated and the results have been compared as statistically. The Damage frequency (%), Arbitrary Unit and Genetic Damage Index (%) were evaluated in gill cells of tilapia. As a result of the study, it is determined that highest the damage frequencies (%) as 69.00±4.58 and 66.00±3.00 were significantly observed in t0 and t6 groups respectively (P<0.001). Besides, it is observed that the other damage parameters (Arbitrary unit and genetic damage ındex) in the gill samples of t0 and t6 groups were significantly higher (P<0.001) compared to the control, t12 and t24 groups. The Arbitrary unit and Genetic damage index increased in fish after the transport process (especially, t6 and t12) but a significant decrease occurred after 24 h (returning to the control levels) (P< 0.001).

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“…В условиях стресса в организме рыб происходит усиленная секреция катехоламинов, таких как адреналин и норадреналин, а также кортизола [8]. Известно, что кортизол способен вызвать подавление иммунитета [9], активировать процессы катаболизма, влиять на ход митотического деления кроветворных клеток [8]. Катехоламины способствуют расщеплению липидов и глюкозы, усиливают тромбоцитопоэз [8].…”

Section: Introductionunclassified

“…Известно, что кортизол способен вызвать подавление иммунитета [9], активировать процессы катаболизма, влиять на ход митотического деления кроветворных клеток [8]. Катехоламины способствуют расщеплению липидов и глюкозы, усиливают тромбоцитопоэз [8].…”

Section: Introductionunclassified

Level of cartisol and parameters of cytogenic homeostasis in fish organism against sporeterm probiotics

Romanova¹,

Спирина²,

Romanov³

et al. 2020

Vestnik of Ulyanovsk SAA

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Ключевые слова: аквакультура, африканский сом, пробиотик споротермин, эритроциты, микроядерный тест, кортизол.С целью повышения продуктивности и экологической чистоты рыбы в технологиях выращивания объектов аквакультуры все чаще используются биологически активные вещества, в том числе пробиотики. Целью работы было исследование уровня кортизола и результатов процесса формирования микроядер в эритроцитах периферической крови африканского сома при выращивании в условиях УЗВ на фоне пробиотика споротермина и без него. Отбор крови осуществляли прижизненно, концентрацию кортизола определяли, используя коммерческие наборы «Кортизол-ИФА». Для проведения микроядерного теста мазки крови фиксировались в фиксаторе Май-Грюнвальда, окрашивались в красителе Романовский-Гимза, производили подсчёт количества клеток с микроядрами на 2000 клеток. Были установлены различия по частоте встречаемости микроядер и их типам между группой рыб, выращенных с использованием споротермина и без него. Было обнаружено достоверное трехкратное превышение концентрации кортизола в крови африканских сомов, при традиционной технологии содержания, по сравнению с содержанием кортизола у рыб, выращенных с использованием споротермина. Использование споротермина привело также к уменьшению частоты встречаемости микроядер в периферической крови клариевых сомов. На фоне споротермина доля клеток с микроядрами типа А снизилось с 2,9 до 1,9. Эритроциты периферической крови с микроядрами типов Б и В при использовании споротермина не обнаруживались. Полученные результаты свидетельствуют, что микроядерный тест может быть успешно использован для прогностической оценки цитогенетического гомеостаза при выращивании рыб в условиях индустриальной аквакультуры.

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The effect of a cortisol analogue and transport stress on the frequency of occurrence of micronuclei in erythrocytes of the peripheral blood of sterlet Acipenser ruthenus L.

Cited by 5 publications

References 3 publications

Electrophoretic Mobility of Red Blood Cells and Micronucleus Test in Exfoliated Buccal Cells as Stress Intensity Markers

Electrophoretic Mobility of Red Blood Cells and Micronucleus Test in Exfoliated Buccal Cells as Stress Intensity Markers

DNA Damage in Hybrid Tilapia (Oreochromis niloticus x O. aureus) Exposed to Short-Transport Process

Level of cartisol and parameters of cytogenic homeostasis in fish organism against sporeterm probiotics

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