The effect of a lytic polysaccharide monooxygenase and a xylanase from  Gloeophyllum trabeum  on the enzymatic hydrolysis of lignocellulosic residues using a commercial cellulase

Sanhueza, Cynthia; Carvajal, Gonzalo; Soto-Aguilar, Javiera; Lienqueo, María Elena; Salazar, Osvaldo

doi:10.1016/j.enzmictec.2017.11.007

Cited by 41 publications

(11 citation statements)

References 55 publications

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“…The same authors also showed that addition of CgAA9 to Celluclast led to a 10% to 20% increase in solubilized reducing sugar obtained from pretreated rice straw. Another recent study showed that a mixture of a xylanase (GH10A) and an LPMO (AA9A) from the fungus Gloeophyllum trabeum (GtGH10A and GtAA9A, respectively) improves the performance of Celluclast (final ratio of Celluclast/GtGH10A/GtAA9A of 60:20:20, wt/ wt/wt) in the saccharification of wheat straw (326). Interestingly, the presence of the GH10 was necessary to unlock the full potential of the AA9.…”

Section: Design Of Enzymatic Cocktails: the Effect Of Oxidoreductasesmentioning

confidence: 99%

Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass

Bissaro

Várnai

Røhr

et al. 2018

Microbiol Mol Biol Rev

240

246

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SUMMARYBiomass constitutes an appealing alternative to fossil resources for the production of materials and energy. The abundance and attractiveness of vegetal biomass come along with challenges pertaining to the intricacy of its structure, evolved during billions of years to face and resist abiotic and biotic attacks. To achieve the daunting goal of plant cell wall decomposition, microorganisms have developed many (enzymatic) strategies, from which we seek inspiration to develop biotechnological processes. A major breakthrough in the field has been the discovery of enzymes today known as lytic polysaccharide monooxygenases (LPMOs), which, by catalyzing the oxidative cleavage of recalcitrant polysaccharides, allow canonical hydrolytic enzymes to depolymerize the biomass more efficiently. Very recently, it has been shown that LPMOs are not classical monooxygenases in that they can also use hydrogen peroxide (H2O2) as an oxidant. This discovery calls for a revision of our understanding of how lignocellulolytic enzymes are connected since H2O2is produced and used by several of them. The first part of this review is dedicated to the LPMO paradigm, describing knowns, unknowns, and uncertainties. We then present different lignocellulolytic redox systems, enzymatic or not, that depend on fluxes of reactive oxygen species (ROS). Based on an assessment of these putatively interconnected systems, we suggest that fine-tuning of H2O2levels and proximity between sites of H2O2production and consumption are important for fungal biomass conversion. In the last part of this review, we discuss how our evolving understanding of redox processes involved in biomass depolymerization may translate into industrial applications.

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Section: Design Of Enzymatic Cocktails: the Effect Of Oxidoreductasesmentioning

confidence: 99%

Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass

Bissaro

Várnai

Røhr

et al. 2018

Microbiol Mol Biol Rev

240

246

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“…The heterogeneous nature of pretreated biomass requires synergistic actions of multiple and functionally different ligno-cellulolytic enzymes (like endoglucanases, exoglucanases, xylanases, mannases, esterases, arabinofuranosidases, etc.) for its efficient hydrolysis (Loman and Ju, 2016;Lee et al, 2017;Kane and French, 2018;Sanhueza et al, 2018). Although a number of factors are responsible for lower biomass hydrolysis high cellulase crystallinity, low enzyme accessibility, non-productive enzyme-lignin binding, competitive, and feedback inhibition of the enzyme have been predominantly reported (Agrawal et al, 2015b;Parnthong et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Development of synergistic enzyme cocktail is an emerging strategy for improving the biomass hydrolysis (Satlewal et al, 2008;Sánchez-Cantú et al, 2018;Sanhueza et al, 2018). It has been reported earlier that both exo and endo glucanases work synergistically to degrade cellulase into cellobiose and finally β-glucosidase hydrolyze cellobiose into glucose.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Enzyme Cocktail to Enhance Hydrolysis of Steam Exploded Wheat Straw at Pilot Scale

et al. 2018

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Multiple enzymes are required for efficient hydrolysis of lignocellulosic biomass and no wild type organism is capable of producing all enzymes in desired levels. In this study, steam explosion of wheat straw was carried out at pilot scale and a synthetic enzyme mixture (EnzMix) was developed by partially replacing the cellulase with critical dosages of commercially available accessory enzymes (β-glucosidase, xylanase and laccase) through central composite design. Highest degree of synergism (DS) was observed with β-glucosidase (1.68) followed by xylanase (1.36). Finally, benchmarking of EnzMix (Celluclast, β-glucosidase and xylanase in a protein ratio of 20.40: 38.43: 41.16, respectively) and other leading commercial enzymes was carried out. Interestingly, hydrolysis improved by 75% at 6 h and 30% at 24 h, respectively in comparison of control. By this approach, 25% reduction in enzyme dosage was observed for obtaining the same hydrolysis yield with opitimized enzyme cocktail. Thus, development of enzyme cocktail is an effective and sustainable approach for high hydrolysis efficiency.

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“…It was discovered that LPMOs promote degradation of the most recalcitrant crystalline cellulose by carrying out oxidative cleavage of polysaccharides [47]. Several studies showed synergetic effects between LPMOs and the lignocellulolytic enzymes during the saccharification process of cellulose and hemicellulose components [9,47]. All CAZymes and non-hydrolyzed protein detected in the secreted proteins of T. harzianum EM0925 are important for fermentable sugars production.…”

Section: Discussionmentioning

confidence: 99%

“…Some researchers found that hemicellulases, oxidoreductases, non-hydrolytic proteins and other auxiliary enzymes synergize to achieve an efficient enzymatic hydrolysis of cellulose [2]. Hemicellulase and pectinase could degrade the non-cellulose polysaccharide that covered cellulose and in turn increase the cellulose hydrolysis efficiency, and this synergistic effect could give rise to the cost reduction in deconstruction of lignocellulose [9]. Goldbeck et al [10] found that when xylanase was added to the commercial cellulase preparation Accellerase 1500, the glucose yield of dilute acid pretreated sugarcane bagasse presented a 1.4-fold increase than that of Accellerase 1500 alone.…”

Section: Introductionmentioning

confidence: 99%

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

Zhang

Yang

Luo

et al. 2020

IJMS

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Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation enzymes. Herein, we report the secretome of Trichoderma harzianum EM0925 under induction of lignocellulose. Numerously and quantitatively balanced cellulases and hemicellulases, especially high levels of glycosidases, could be secreted by T. harzianum EM0925. Compared with the commercial enzyme preparations, the T. harzianum EM0925 enzyme cocktail presented significantly higher lignocellulolytic enzyme activities and hydrolysis efficiency against lignocellulosic biomass. Moreover, 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding and alkali pretreated corn stover. These findings demonstrate a natural cellulases and hemicellulases mixture for complete conversion of biomass polysaccharide, suggesting T. harzianum EM0925 enzymes have great potential for industrial applications.

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The effect of a lytic polysaccharide monooxygenase and a xylanase from Gloeophyllum trabeum on the enzymatic hydrolysis of lignocellulosic residues using a commercial cellulase

Cited by 41 publications

References 55 publications

Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass

Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass

Synergistic Enzyme Cocktail to Enhance Hydrolysis of Steam Exploded Wheat Straw at Pilot Scale

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

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