The effect of adding Bacillus amyloliquefaciens LSG2-8 in diets on the growth, immune function, antioxidant capacity, and disease resistance of Rhynchocypris lagowskii

Yu, Mengnan; Zhang, Yurou; Zhang, Dongming; Wang, Qiuju; Wang, Guiqin; Elsadek, Mahmound; Yao, Qi; Chen, Yuke; Guo, Zhixin

doi:10.1016/j.fsi.2022.05.008

Cited by 8 publications

(3 citation statements)

References 56 publications

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“…Bacillus amyloliquefaciens LSG2-8 could significantly increase the growth parameters of R. lagowskii, such as weight gain rate, final body weight, feed efficiency and specific growth rate. Bacillus amyloliquefaciens LSG2-8 ISSN (print): 1823-8262, ISSN (online): 2231-7538 could improve the growth performance, antioxidant capacity, immune function and disease resistance of R. lagowskii and be used in aquaculture (Yu et al, 2022). Bacillus amyloliquefaciens LSG2-8 may improve the intestinal barrier function of R. lagowskii and can be used as a feed additive in aquaculture (Yu et al, 2023).…”

Section: Resultsmentioning

confidence: 99%

Pathogenicity analysis and application of probiotic bacteria in Catfish (Clarias sp.) cultivation in vivo

Sukmawati, S.,

Fahrizal, A.,

Yunita, M.

2024

MJM

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Aims:The aim of this study was to analyze the physiological properties, pathogenicity and application of probiotic bacteria to catfish (Clarias sp.) cultivation in vivo. Methodology and results: Probiotic bacteria's physiological characteristics were described using descriptive approaches, whereas experimental methods analyzed pathogenicity tests and the application of the probiotic bacteria in catfish cultivation. The results of bacterial characterization showed that Bacillus safensis strain C251 SA3, Bacillus amyloliquefaciens strain NO10 SA8 and Bacillus australimaris strain IIHR GAPB01 SL1 had bacili cell shape and showed a positive catalase test for all test bacteria except Bacillus safensis strain C251 SA3. The three probiotic bacteria also had negative oxidative-fermentative results, which were able to reduce nitrate and survive in environmental conditions with pH 3-9. Pathogenicity analysis revealed that the three bacteria had the potential to be non-pathogenic or less pathogenic. Bacillus safensis strain C251 SA3 treatment on catfish resulted in the best survival rate of 64% and a 17.5 cm growth in catfish body length by week 13. When Bacillus amyloliquefaciens strain NO10 SA8 was applied to catfish, the survival rate was 32% and the catfish body length increased by 22 cm by week 13. The control treatment revealed that all catfish samples died or had a 0% survival rate, while the application of Bacillus australimaris strain IIHR GAPB01 SL1 revealed a catfish survival rate of 0%. Conclusion, significance and impact of study: Bacillus australimaris strain IIHR GAPB01 SL1 is a potential probiotic bacterium to be applied in catfish food.

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Section: Resultsmentioning

confidence: 99%

Pathogenicity analysis and application of probiotic bacteria in Catfish (Clarias sp.) cultivation in vivo

Sukmawati, S.,

Fahrizal, A.,

Yunita, M.

2024

MJM

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“…In this experiment, the activity of lysozyme (LZM) in the blood of the FC8 group significantly increased ( P < 0.05), while there was no significant difference between the liver control and the experimental groups ( P > 0.05), which was similar to the results of feeding juvenile cobia with Bacillus subtilis . The increase in lysozyme activity by Bacillus may be due to its cell wall components or the active substances produced by Bacillus , which stimulate the host intestinal immune cells to enhance the expression of related immune genes, thus enabling the host to improve the innate immune capacity of the body [ 48 ]. The results of this study indicate that Bacillus halophilus can improve the non-specific immunoenzyme activity of large yellow croakers, but the optimal supplemental level needs to be further studied.…”

Section: Discussionmentioning

confidence: 99%

Effects of Bacillus halophilus on growth, intestinal flora and metabolism of Larimichthys crocea

Huang,

Shui,

Wang

et al. 2023

Biochemistry and Biophysics Reports

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“…Wang et al explained the effects of Bacillus (10 8 ~10 9 CFU/g) on the survival rate and molting of L. vannamei larvae [33]. B. amyloliquefaciens significantly improved the WG, SGR, and FCR of O. niloticus [34]. B. amyloliquefaciens LSG2-8 added to Rhynchocypris lagowskii feed significantly improved FBM, WGR, SGR, and FCR growth indices [35].…”

Section: Introductionmentioning

confidence: 99%

Effects of Dietary Gracilaria lichenoides and Bacillus amyloliquefaciens on Growth Performance, Antioxidant Capacity, and Intestinal Health of Penaeus monodon

Tian,

Wang,

Huang

et al. 2024

Biology

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This research sought to assess the effects of dietary supplements with Gracilaria lichenoides and Bacillus amyloliquefaciens, either individually or combined, on the growth performance, antioxidant capacity, and intestinal function of Penaeus monodon. A total of 840 shrimps were randomly assigned to 28 tanks with an average initial weight of (1.04 ± 0.03) g (30 shrimp per tank) with 7 different treatment groups and 4 replicates per treatment. The control treatment (C) consisted of a basal diet; in contrast, the experimental groups were complement with varying levels of G. lichenoides (3% or 8%), either alone (S3 and S8) or in combination with B.amyloliquefaciens at different concentrations (3% G. lichenoides and 109 CFU/g—S3B9; 8% G. lichenoides and 1011 CFU/g B. amyloliquefaciens—S8B11; 109 CFU/g B. amyloliquefaciens—S9; 1011 CFU/g B. amyloliquefaciens—B11). The results indicated that the maximum values of final body weight (FBW) (10.49 ± 0.90) g, weight gain rate (WGR) (908.94 ± 33.58) g, and specific growth rate (SGR) (4.20 ± 0.06) g were perceived in the 3% G. lichenoide diet treatment, and compared with the control group, the difference was significant (p < 0.05). The whole-body lipid content of shrimp in the B9 group was significantly higher than that in the B11 group (p < 0.05), but no significant difference was observed when compared with shrimp fed other diets (p > 0.05). The ash content of shrimp in the B9 group was found to be significantly higher than that in the S3B9 group (p < 0.05). Furthermore, the lipase activity in the stomach and intestines of the experimental groups exhibited a statistically significantly increase compared to the control (p < 0.05). In comparison to the control group, the hepatopancreas of the S3 group exhibited a significant increase in the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and antioxidant genes [SOD, catalase (CAT), GSH-Px, thioredoxin (Trx), Hippo, and NF-E2-related factor 2 (Nrf2)] expression levels (p < 0.05). Additionally, the activities of total antioxidant capacity (T-AOC), SOD, peroxidase (POD), and antioxidant genes (CAT, GSH-Px, Trx, and Hippo) in the S3B9 treatment of hepatopancreas showed significant improvement (p < 0.05). The inclusion of dietary G. lichenoides and B. amyloliquefaciens resulted in enhanced relative expression of intestinal lipid metabolism genes (fatty acid synthetase (FAS), lipophorin receptor (LR), fatty acid transport protein 1 (FATP1)) and suppressed the expression of the long-chain fatty acid-CoA ligase 4 (LCL4) gene. Analysis of microbiota sequencing indicated improvements in composition and structure, with notable increases in Firmicutes at the phylum level and Vibrio at the genus level in the S3 group, as well as an increase in Tenericutes at the genus level in the S8B11 group. Overall, the inclusion of dietary G. lichenoides and B. amyloliquefaciens positively impacted the growth, antioxidant capacity, and microbial composition of shrimp, with particular enhancement observed in shrimp fed a supplementary 3% G. lichenoides diet.

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The effect of adding Bacillus amyloliquefaciens LSG2-8 in diets on the growth, immune function, antioxidant capacity, and disease resistance of Rhynchocypris lagowskii

Cited by 8 publications

References 56 publications

Pathogenicity analysis and application of probiotic bacteria in Catfish (Clarias sp.) cultivation in vivo

Pathogenicity analysis and application of probiotic bacteria in Catfish (Clarias sp.) cultivation in vivo

Effects of Bacillus halophilus on growth, intestinal flora and metabolism of Larimichthys crocea

Effects of Dietary Gracilaria lichenoides and Bacillus amyloliquefaciens on Growth Performance, Antioxidant Capacity, and Intestinal Health of Penaeus monodon

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