The effect of agglomeration state of silver and titanium dioxide nanoparticles on cellular response of HepG2, A549 and THP-1 cells

Lankoff, Anna; Sandberg, Wiggo J.; Węgierek-Ciuk, Aneta; Lisowska, Halina; Refsnes, Magne; Sartowska, B.; Schwarze, Per E.; Męczyńska-Wielgosz, Sylwia; Wojewódzka, Maria; Kruszewski, Marcin

doi:10.1016/j.toxlet.2011.11.006

Cited by 217 publications

(191 citation statements)

References 56 publications

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“…22 Compared to nonphagocytic cells, THP-1 cells are also much more resistant to 20-200 nm silver and to 21 nm TiO 2 NPs. 23 Size-dependent toxicity studies in vivo are less conclusive: systemic toxicity upon intraperitoneal application of 10 nm and 50 nm iron oxide particles was higher than that after dosing with 1000 nm particles. 24 When applied intraocularly however, 4000 nm magnetic iron oxide particles caused more toxicity than 50 nm particles.…”

Section: Cytotoxicitymentioning

confidence: 99%

The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles

Frőhlich¹

2012

IJN

2,013

1,416

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Many types of nanoparticles (NPs) are tested for use in medical products, particularly in imaging and gene and drug delivery. For these applications, cellular uptake is usually a prerequisite and is governed in addition to size by surface characteristics such as hydrophobicity and charge. Although positive charge appears to improve the efficacy of imaging, gene transfer, and drug delivery, a higher cytotoxicity of such constructs has been reported. This review summarizes findings on the role of surface charge on cytotoxicity in general, action on specific cellular targets, modes of toxic action, cellular uptake, and intracellular localization of NPs. Effects of serum and intercell type differences are addressed. Cationic NPs cause more pronounced disruption of plasma-membrane integrity, stronger mitochondrial and lysosomal damage, and a higher number of autophagosomes than anionic NPs. In general, nonphagocytic cells ingest cationic NPs to a higher extent, but charge density and hydrophobicity are equally important; phagocytic cells preferentially take up anionic NPs. Cells do not use different uptake routes for cationic and anionic NPs, but high uptake rates are usually linked to greater biological effects. The different uptake preferences of phagocytic and nonphagocytic cells for cationic and anionic NPs may influence the efficacy and selectivity of NPs for drug delivery and imaging.

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Section: Cytotoxicitymentioning

confidence: 99%

The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles

Frőhlich¹

2012

IJN

2,013

1,416

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“…The literature reports opposite effects, either demonstrating higher resistance of differentiated THP-1 cells [42,43] or establishing lower susceptibility for A549 cells [44], clearly indicating that the generated responses are strongly dependent on the assessed materials. What is clearly seen in our study is that there was a difference of susceptibilities when testing free drugs and polymer, which are exposed as solutions, comparing with microparticles.…”

Section: Evaluation Of Metabolic Activitymentioning

confidence: 99%

“…This different outcome is probably related with specific endocytic-exocytic mechanisms of phagocytic and non-phagocytic cells, along with the specialized physiological role of each particular cell type. THP-1 cells are phagocytes with significant endocytic and exocytic activity and natural ability to uptake particulate matter [43]. Therefore, they possibly respond with higher intensity to the ingestion of particulates [44].…”

Section: Evaluation Of Metabolic Activitymentioning

confidence: 99%

Inhalable Antitubercular Therapy Mediated by Locust Bean Gum Microparticles

et al. 2016

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Tuberculosis remains a major global health problem and alternative therapeutic approaches are needed. Considering the high prevalence of lung tuberculosis (80% of cases), the pulmonary delivery of antitubercular drugs in a carrier system capable of reaching the alveoli, being recognised and phagocytosed by alveolar macrophages (mycobacterium hosts), would be a significant improvement to current oral drug regimens. Locust bean gum (LBG) is a polysaccharide composed of galactose and mannose residues, which may favour specific recognition by macrophages and potentiate phagocytosis. LBG microparticles produced by spray-drying are reported herein for the first time, incorporating either isoniazid or rifabutin, first-line antitubercular drugs (association efficiencies >82%). Microparticles have adequate theoretical properties for deep lung delivery (aerodynamic diameters between 1.15 and 1.67 µm). The cytotoxic evaluation in lung epithelial cells (A549 cells) and macrophages (THP-1 cells) revealed a toxic effect from rifabutin-loaded microparticles at the highest concentrations, but we may consider that these were very high comparing with in vivo conditions. LBG microparticles further evidenced strong ability to be captured by macrophages (percentage of phagocytosis >94%). Overall, the obtained data indicated the potential of the proposed system for tuberculosis therapy.

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“…Indeed, endocytosis represents one of the main internalization mechanisms of NP in cells, 11,15,23 particularly in macrophages. 23,24 For the quantification of particles associated with the plasma membranes and internalized into the cytoplasm, cells were exposed to BFO-NP, fixed and labelled with a fluorescent probe used to stain membranes and lipids. 25 Exposure time of 2 h and 24 h were chosen since considered representative minimum and maximum exposure time for diagnostic applications; whereas 72 h was identified as a critical time point for cytotoxicity response.…”

Section: Interaction Between Bfo-np and Human Derived Cellsmentioning

confidence: 99%

Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles

Staedler

Passemard

Magouroux

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

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Bismuth Ferrite (BFO) nanoparticles (BFO-NP) display interesting optical (nonlinear response) and magnetic properties which make them amenable for bio-oriented diagnostic applications as intra-and extra membrane contrast agents. Due to the relatively recent availability of this material in well dispersed nanometric form, its biocompatibility was not known to date. In this study, we present a thorough assessment of the effects of in vitro exposure of human adenocarcinoma (A549), lung squamous carcinoma (NCI-H520), and acute monocytic leukemia (THP-1) cell lines to uncoated and poly(ethylene glycol)-coated BFO-NP in the form of cytotoxicity, haemolytic response and biocompatibility. Our results support the attractiveness of the functional-BFO towards biomedical applications focused on advanced diagnostic imaging.

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The effect of agglomeration state of silver and titanium dioxide nanoparticles on cellular response of HepG2, A549 and THP-1 cells

Cited by 217 publications

References 56 publications

The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles

The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles

Inhalable Antitubercular Therapy Mediated by Locust Bean Gum Microparticles

Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles

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