The effect of aggregation state of amphotericin-B on its interactions with cholesterol- or ergosterol-containing phosphatidylcholine monolayers

Barwicz, Joanna; Tancrède, Pierre

doi:10.1016/s0009-3084(96)02652-7

Cited by 97 publications

(71 citation statements)

References 38 publications

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“…49,50 As shown in Figure 9D, the interaction of amphotericin B with gelatin resulted in a significant decrease in the hemolysis of rabbit erythrocytes compared to free drug at 32 µg/mL and 64 µg/mL. Similarly, interaction of natamycin with gelatin also resulted in decreased hemolysis at 64 µg/mL.…”

Section: Hemolytic Activity Of Antifungalloaded Gelatinmentioning

confidence: 86%

Interaction of gelatin with polyenes modulates antifungal activity and biocompatibility of electrospun fiber mats

Lakshminarayanan

Sridhar

Loh

et al. 2014

IJN

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Topical application of antifungals does not have predictable or well-controlled release characteristics and requires reapplication to achieve therapeutic local concentration in a reasonable time period. In this article, the efficacy of five different US Food and Drug Administration-approved antifungal-loaded (amphotericin B, natamycin, terbinafine, fluconazole, and itraconazole) electrospun gelatin fiber mats were compared. Morphological studies show that incorporation of polyenes resulted in a two-fold increase in fiber diameter and the mats inhibit the growth of yeasts and filamentous fungal pathogens. Terbinafine-loaded mats were effective against three filamentous fungal species. Among the two azole antifungals compared, the itraconazole-loaded mat was potent against Aspergillus strains. However, activity loss was observed for fluconazole-loaded mats against all of the test organisms. The polyene-loaded mats displayed rapid candidacidal activities as well. Biophysical and rheological measurements indicate strong interactions between polyene antifungals and gelatin matrix. As a result, the polyenes stabilized the triple helical conformation of gelatin and the presence of gelatin decreased the hemolytic activity of polyenes. The polyene-loaded fiber mats were noncytotoxic to primary human corneal and sclera fibroblasts. The reduction of toxicity with complete retention of activity of the polyene antifungal-loaded gelatin fiber mats can provide new opportunities in the management of superficial skin infections.

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Section: Hemolytic Activity Of Antifungalloaded Gelatinmentioning

confidence: 86%

Interaction of gelatin with polyenes modulates antifungal activity and biocompatibility of electrospun fiber mats

Lakshminarayanan

Sridhar

Loh

et al. 2014

IJN

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“…This explains the fact that the affinity of the antibiotic or its derivatives towards ergosterol was found to be higher as compared to cholesterol [18,21,43,44]. …”

Section: Accepted M Manuscriptmentioning

confidence: 98%

“…Mammalian membranes contain high proportion of phosphatidylcholines (PC), both saturated and unsaturated [17]. As regards sterols, mammalian cellular membrane contains 30% of cholesterol as compared to 13% of ergosterol in fungi membrane [18]. Therefore, we have constructed model membranes containing either 13% or 30% of a sterol (ergosterol or cholesterol) and a model phospholipd (saturated: DPPC and unsaturated: DOPC).…”

mentioning

confidence: 99%

Interactions between membrane sterols and phospholipids in model mammalian and fungi cellular membranes — A Langmuir monolayer study

Miñones

País

Conde

et al. 2009

Biophysical Chemistry

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To cite this version:J. Miñones, S. Pais, O. Conde, P. Dynarowicz-Łątka. Interactions between membrane sterols and phospholipids in model mammalian and fungi cellular membranes -A Langmuir monolayer study. Biophysical Chemistry, Elsevier, 2009, 140 (1-3) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D

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“…The use of AmB is limited by several side effects, including nephrotoxicity and hemolytic activity; therefore much effort has been made to develop formulations able to reduce its toxicity and increase its therapeutic effect (Brajtburg and Bolard 1996;Torrado et al 2008). Both the therapeutic and toxic effects of AmB seem to be related to its state of aggregation, and the monomeric state of AmB is usually ascribed as the less toxic state (LamyFreund et al 1989;Bolard et al 1991;Barwicz and Tancrede 1997;Barwicz et al 1992;Legrand et al 1992). In this context, sonicated DODAB bicelles have been found to be able to solubilize mainly monomeric AmB molecules (Vieira and Carmona-Ribeiro 2001) and display low nephrotoxicity (Lincopan et al 2005).…”

Section: Detecting Dodab Bicelles By Esrmentioning

confidence: 99%

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

et al. 2017

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Cationic bilayers have been used as models to study membrane fusion, templates for polymerization and deposition of materials, carriers of nucleic acids and hydrophobic drugs, microbicidal agents and vaccine adjuvants. The versatility of these membranes depends on their structure. Electron spin resonance (ESR) spectroscopy is a powerful technique that employs hydrophobic spin labels to probe membrane structure and packing. The focus of this review is the extensive structural characterization of cationic membranes prepared with dioctadecyldimethylammonium bromide or diC14-amidine to illustrate how ESR spectroscopy can provide important structural information on bilayer thermotropic behavior, gel and fluid phases, phase coexistence, presence of bilayer interdigitation, membrane fusion and interactions with other biologically relevant molecules.

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The effect of aggregation state of amphotericin-B on its interactions with cholesterol- or ergosterol-containing phosphatidylcholine monolayers

Cited by 97 publications

References 38 publications

Interaction of gelatin with polyenes modulates antifungal activity and biocompatibility of electrospun fiber mats

Interaction of gelatin with polyenes modulates antifungal activity and biocompatibility of electrospun fiber mats

Interactions between membrane sterols and phospholipids in model mammalian and fungi cellular membranes — A Langmuir monolayer study

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

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