The Effect of Antibody on the Intestinal Absorption of Macromolecules and on Intestinal Permeability in Adult Mice

Abstract

Oral immunization was shown to reduce the passage of a bacterial antigen (BA) through the intestinal wall. The extent of the reduction found was dependent on the technique used for antigen assay, but with ¹²⁵I-BA in an everted gut sac system, the amount of transported antigen was less than 60% of the amount in a control non-immune system. The inhibitory effect was due to coproantibodies which probably function by complexing and trapping the antigen in the mucous layer. This inhibition of antigen transport seems a prerogative of IgA antibodies since serum-derived antibodies caused concomitant adverse effects.

Early Dietary Antigens Delay the Development of Gut Mucosal Barrier in Preweaning Rats

“…The decrease in intestinal uptake of antigen by preexisting antibodies may be highly antigen specific, because enhanced penetration of unrelated macromolecules has been demonstrated to occur simultaneously (27,28). The dams in the present study did not have immune experience of the antigens investigated.…”

Intestinal Absorption of Bacterial Antigens in Normal Adult Mice

“…It is as if some of the antigen had leaked in! However, this is unlike ly, since a decreased absorption was seen in immune gut sacs previously [22]. Whatever the explanation, re sults from in vitro models must consequently be inter preted with caution.…”

“…In this study, 0.08-0.09% of the BA adminis tered was estimated to be absorbed under the experi mental conditions, in both GF and SPF mice. The technique seemed reliable since antibodies given with the antigen inhibited its absorption [22].…”

Immunoglobulin levels in bronchoalveolar lavage fluid of children with chronic chest disease

“…This has been suggested by experimental in vitro and in vivo models in which IgG antibodies to one antigen facilitate mucosal penetration of bystander proteins. 28,29 In addition, capillary leakage in asthmatic inflammation may be enhanced when local immune complexes, formed by complement activating IgG-1 and IgG-3 antibodies directed against dietary constituents and infectious agents, attract polymorphonuclear granulocytes. High concentrations of immunoglobulins may be harmful rather than helpful in patients with chronic lung disease.…”