The effect of aqueous leaf extract of fluted pumpkin on some hematological parameters and liver enzymes in 2,4-dinitrophenylhydrazine- induced anemic rats

Abstract

Anemia constitutes a serious health problem in many tropical countries including Nigeria because of the prevalence of malaria and other parasitic infections which possibly leads to decrease of hemoglobin. Fluted pumpkin has been reported to be very good in building the constituents of the blood and also replacing them. This study was designed to investigate the effects of the aqueous leaf extract of fluted pumpkin on some hematological parameters and liver enzymes; alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in 2, 4-dinitrophenylhydrazine-induced anemia in experimental rat. Twelve Wister albino male rats were obtained from a nearby farm and separated into four groups of three rats each for the study. Rats in Groups 1 and 2 were injected 40 mg/kg 2, 4-dinitrophenylhydrazine for eight days to induce anemia, while rats in Groups 3 and 4 were fed with grower's mash and water ad libitum. All analysis was done using the standard methods. The result of this study shows that, oral administration of 50 mg/kg of aqueous leaf extract of fluted pumpkin to the rats in induced treated and normal treated groups (Groups 1 and 3), increased the hematological parameters under investigation while the rest remained significantly unchanged. Low level of ALT and AST was observed in rats in group 1 (induced treated group) suggesting a hepatoprotective property of the leaf extract which also indicate that the extract had no effect on the liver of the rats at the concentration used. The result of this research indicate that 50 mg/kg aqueous leaf extract of fluted pumpkin could elevate the packed cell volume, red blood cells and hemoglobin concentration in the rats induced with 2, 4-dinitrophenylhydrazine. Hence, oral administration of the extract could cure hemolytic anemia. The leaf extract also regulated the liver enzymes (ALT and AST) of the rats induced with 2, 4-dinitrophenylhydrazine. It can be concluded that aqueous leaf extract of fluted pumpkin is a potential blood booster and has hepatoprotective property.

Effect of ethanol extract of Telfairia occidentalis leaf on some biochemical parameters of 2,4-dinitrophenyl hydrazine induced oxidative stress in albino rats

“…The induction of the rats resulted to the elevation of the levels of ALT and ALP significantly. This observation agrees with that of Toma et al [25].…”

Effects of Methanol Seed Extract of Aframomum melegueta (Alligator Pepper) on Wistar Rats with 2,4-Dinitrophenylhydrazine Induced Hemolytic Anemia

“…This metabolic status can only be ensured by decrease in RBC destruction or increased RBC production [16]. The increase in hematological parameters investigated could be as a result of some constituents such as iron and some B-complex vitamin that the plant extract possess as these serves as hematopoietic factors that influence directly blood cells production in the bone marrow [13,17]. The results of this study were consistent with the observations of the authors who had reported significant increase in hematological parameters of 2,4-dinitrophenylhydrazine-induced anemic rats treated with various natural products such as fluted pumpkin [13,18].…”

“…As observed in this study, the standard drug (astifer) had some positive effect on the production of blood cells in the tested rats; this was observed by the increase in PCV, HB, RBC, WBC, and neutrophils concentration of anemic rats after the administration of the standard drug at 20 ml/kg b.w. and the increase in WBC could be due to the defense mechanism against the entrance of foreign material in the body system of the rats [13]. The increase in PCV is an indication of increase hemoglobin concentration that may result from increased RBC count [14].…”

Erythrocyte Formation Rate in Wistar Anemia Induced 2,4-Dinitrophenylhydrazine through Intake Maize Biofortified Iron

“…Each group consisted of five animals that were placed separately in a 30 cm × 25 cm × 30 cm cage. The treatment group of Wistar anemia was supplied food from MBI 3 times a day as well as a control group by maize non-biofortified iron, and water drinking by ad libitum [12]. In addition, Wistar anemia was treated well in a room with good ventilation, normal sun exposure through the window, temperature 27-30°C, and low humidity.…”